[1]张丽芳,王继华,张艺萍,等.西花蓟马人工繁殖技术[J].黑龙江农业科学,2017,(08):57-59.[doi:10.11942/j.issn1002-2767.2017.08.0057]
　ZHANG Li-fang,WANG Ji-hua,ZHANG Yi-ping,et al.Study on Artificial Reproduction Technology of Frankliniella occidentalis (Pergande)[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(08):57-59.[doi:10.11942/j.issn1002-2767.2017.08.0057]
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西花蓟马人工繁殖技术

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年08期 页码: 57-59 栏目: 出版日期: 2017-08-10
Title:
Study on Artificial Reproduction Technology of Frankliniella occidentalis (Pergande)
文章编号:
1002-2767（2017）08-0057-03
作者:
张丽芳1; 王继华1; 张艺萍1; 杨秀梅1; 杨少杰2; 许凤1
1.云南省农业科学院 花卉研究所，云南 昆明 650205；2.云南省科学技术院，云南 昆明 650228
Author(s):
ZHANG Li-fang1; WANG Ji-hua1; ZHANG Yi-ping1; YANG Xiu-mei1; YANG Shao-jie2; XU Feng1
1.Flower Research of Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205；2.Yunnan Academy of Science and Technology，Kunming,Yunnan 650228
关键词:
西花蓟马; 香石竹组培苗; 人工繁殖
Keywords:
Frankliniella occidentalis;  Dianthus caryophyllu tissue culture seedlings; artificial reproduction technology
分类号:
S433.89
DOI:
10.11942/j.issn1002-2767.2017.08.0057
文献标志码:
B
摘要:
为了获得大量虫龄整齐、发育一致的西花蓟马若虫及成虫，开展了西花蓟马室内人工繁殖技术的研究。通过形态学方法鉴定出西花蓟马成虫，将成虫转接至香石竹植株产卵48 h，以产卵后的香石竹茎段为材料，接入培养基，获取清洁虫源。结果表明：常规组培方法生产出的香石竹组培苗作为寄主，将获得的清洁虫源接种到寄主上，在室内已连续饲养3代，平均存活率均超过80%，完成1代大约需要14 d。此方法可用于室内进行西花蓟马种群的建立和繁殖，为综合防治技术的研究提供大量虫源。
Abstract:
Indoor artificial reproduction technology of Frankliniella occidentalis was developed to obtain a large number of F.occidentalis nymphs and adults with uniform instar and consistent development.F.occidentalis adults were identified with the morphological method and transferred to Dianthus caryophyllu plants for oviposition for 48 h.Then the D.caryophyllus stems with egg were inoculated in the culture medium to obtain the clean insect source.After that,clean insect source was inoculated on the tissue culture seedlings of D.caryophyllus which was produced with the conventional tissue culture method for continuous three generation culture indoor,with the survival rate of 80% and 14 d for each generation.This method could be used for the construction and reproduction of F.occidentalis population,providing a large number of insects for the study of its comprehensive control.

参考文献/References:

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备注/Memo

收稿日期：2017-06-13基金项目：重点新产品开发专项（农业）资助项目（2016BB009）；科技创新强省计划资助项目（2014AB014）第一作者简介：张丽芳（1977-），女，云南省富源县人，硕士，副研究员，从事观赏花卉质量控制研究。E-mail：1021619552@qq.com。通讯作者：王继华（1973-），男，云南省文山州人，博士，研究员，从事观赏植物病理学研究。E-mail：wang0505@21cn.com。

更新日期/Last Update: 2017-12-08