[1]贺伟强,陈召桂,高璇,等.响应面法优化箬叶总黄酮的超声提取工艺[J].黑龙江农业科学,2016,(11):131-135.[doi:10.11942/j.issn1002-2767.2016.11.0131]
　HE Wei-qiang,CHEN Zhao-gui,GAO Xuan,et al.Optimization of Ultrasound-assisted Extraction of Total Flavonoids from Indocalmus Leaves[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(11):131-135.[doi:10.11942/j.issn1002-2767.2016.11.0131]
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响应面法优化箬叶总黄酮的超声提取工艺

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2016年11 页码: 131-135 栏目: 出版日期: 2016-11-10
Title:
Optimization of Ultrasound-assisted Extraction of Total Flavonoids from Indocalmus Leaves
文章编号:
11-0131-05
作者:
贺伟强1; 陈召桂2; 高璇1; 洪丽芝1
（1.嘉兴职业技术学院，浙江 嘉兴 314001；2. 浙江五芳斋实业股份有限公司，浙江 嘉兴314000）
Author(s):
HE Wei-qiang1; CHEN Zhao-gui2; GAO Xuan1; HONG Li-zhi1
(1.Jiaxing Vocational and Technical College,Jiaxing,Zhejiang 314001；2. Zhejiang Wufangzhai Industry Limited Company,Jiaxing，Zhejiang 314000)
关键词:
箬叶; 总黄酮; 提取工艺; 响应面法
Keywords:
indocalmus leaves;  total flavonoids;  extraction technology;  response surface methodology
分类号:
S795.9
DOI:
10.11942/j.issn1002-2767.2016.11.0131
文献标志码:
A
摘要:
为了优化箬叶黄酮的提取工艺，在乙醇体积分数、液料比、提取时间和超声功率4个单因素试验的基础上，采用响应面法优化箬叶总黄酮的提取工艺，考察各因素的影响效应及其交互作用。结果表明：确定最佳提取工艺条件为液料比 50∶1，乙醇体积分数76%，提取时间80 min，超声功率234 W，提取2次。采用此工艺条件，箬叶总黄酮得率预测值为2.052%，验证值为2.017%。回归方程的预测值与验证值的相对误差为1.71%，该回归方程与实际情况拟合良好。
Abstract:
In order to optimize flavonoids extraction process from indocalmus leaves，based on single factor test，response surface methodology was used to explore the factors of ethanol concentration，liquid-material ratio，extraction time and ultrasonic power as well as their cross-interactions on extraction rate of flavonoids. Based on the results of response surface methodology,the optimum extraction conditions was ascertained as follows: liquid-material ration was 50∶1,ethanol concentration was 76%,extraction time was 80 min and ultrasonic power was 234 W,extraction twice.Under the optimum extraction condition,the predicted extraction rate of flavonoids was 2.052% and the actual extraction rate was 2.017%. The experimental values predicted from the regression equation within a relative error of 1.71%,indicating a good fitness.

参考文献/References:

[1] 苏春花 , 王福升 , 丁雨龙 . 长节箬竹叶总黄酮提取工艺 [J]. 林业科技开发 ,2010,24(5):87-90.

[2]高梦祥,张佳烂,王江明.微波浸提竹叶黄酮的工艺研究[J].西北农林科技大学学报：自然科学版，2005,33（7）:147-150.

[3]黄京华.丛生竹黄酮类化合物的分析研究及其在分类上的应用[J].竹子研究汇刊，1993,12(1):18-27.

[4]魏凤玉,方菊,陈玮,等.竹叶黄酮在豆浆保鲜中的应用[J].食品科学，2012,33(18):312-315.

[5]崔大明,张益波,付璐,等.响应面法优化超声法提取枸杞中总黄酮工艺[J].食品研究与开发，2011,32(3):55-59.

[6]Myers W R.Encycloedia of biopharmaceutical statistics[M].New York：Marcel Dekker,2003:858-869.

[7]陈魁.试验设计与分析[M].北京:清华大学出版社,1996:94-180.

[8]吴华勇,黄赣辉,顾振宇,等.响应曲面法优化竹叶总黄酮的提取工艺研究[J].食品科学,2008,29（11）:196-200.

[9]余清,许慧星,肖小蓉,等.基于响应面分析法优化的乌饭树叶总黄酮提取条件[J].食品科学,2008,24(1):93-98.

[10]肖卫华,韩鲁佳,杨增玲,等.响应面法优化黄芪黄酮提取工艺的研究[J].中国农业大学学报,2007,12(5):52-56.

[11]邹建国,刘飞,刘燕燕,等.响应面法优化微波辅助提取枳壳中总黄酮工艺[J].食品科学,2012,33(2):24-28.

[12]候学敏,李林霞,张直峰,等.响应面法优化薄荷叶总黄酮提取工艺及抗氧化活性[J].食品科学,2013,34(6):124-128.

[13]贺伟强,周奇迹,沈永根.微波辅助水浸提桑叶总黄酮的工艺条件优化试验[J].蚕业科学,2014,40(2):295-300.

[14]王丰俊,王运强,顾欣,等.响应面法优化超声波提取构树叶中叶绿素的工艺研究[J].北京林业大学学报,2010,32(6)：135-140.


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备注/Memo

收稿日期：2016-07-16

基金项目：2015年浙江省高校访问工程师资助项目（2015〔66〕）
第一作者简介：贺伟强（1979-），男，河南省新郑市人，硕士，副教授，从事植物活性成分的提取与开发技术研究。E-mail：hwq2005003@163.com。

更新日期/Last Update: 2017-01-21