WANG Miao,WU Chun-hua,SUN Li-na.Establishment of Highly Efficient Propagation System of Begonia tuberhybrieda Vosa[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(11):16-19.[doi:10.11942/j.issn1002-2767.2016.11.0016]
球根海棠金正日花高效繁殖体系的建立
- Title:
- Establishment of Highly Efficient Propagation System of Begonia tuberhybrieda Vosa
- 文章编号:
- 11-0016-03
- 分类号:
- S682
- 文献标志码:
- A
- 摘要:
- 为促进秋海棠金正日花的规模化生产，以球根海棠(Begonia tuberhybrieda Vosa) 金正日花的叶片为外植体，研究植物细胞分裂素6-BA、ZT、TDZ和生长素NAA不同种类和浓度对金正日花试管苗增殖的影响。结果表明：最适诱导培养基是MS+6-BA1.5 mg·L-1+NAA0.2 mg·L-1，愈伤组织诱导率达100%，不定芽诱导数达7.2；最佳丛生芽分化培养基为MS+6-BA1.5 mg·L-1+NAA0.2 mg·L-1，增殖系数达8.32；最适生根培养基是MS+NAA0.3 mg·L-1，生根率为100%。
- Abstract:
- In order to promote the large-scale production of Begonia tuberhybrieda Vosa,taking the leaves of Begonia tuberhybrieda Vosa as explant,different concentrations of kinetin,6-BA,ZT,TDZ and NAA was added to MS medium to optimize the culture medium ,and the tissue culture and plant regeneration were studied. The optimal medium for inducing callus was MS+6-BA1.5 mg·L-1+NAA0.2 mg·L-1,which the inductivity could reach to 100%，abventitious bud induction coefficient achieved at 7.2.The shoots specialized perfectly on medium of MS+6-BA1.5 mg·L-1+NAA0.2 mg·L-1,for the growth coefficient achieved at 8.32.The best medium for rooting was MS+NAA0.3 mg·L-1,which rooting rate approached to 100%.
参考文献/References:
[1]
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[2]史銮章.球根海棠组织培养试验研究初报[J]. 农业与技术,2014(3):21. [3]宋仪农.球根秋海棠金正日花组织培养再生体系的研究[D].泰安：山东农业大学,2007. [4]周余华,祁李发. 球根海棠优化组培技术研究[J].安徽农业科学,2003,31(5):733,735.
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备注/Memo
收稿日期：2016-09-02