[1]徐清华,吴旭飞,БРЫКСИН Д.М.,等.过氧乙酸离析法观测蓝靛果忍冬叶片脉序的方法研究[J].黑龙江农业科学,2016,(03):80-83,105.[doi:10.11942/j.issn1002-2767.2016.03.0080]
　XU Qing-hua,WU Xu-fei,..,et al.Observation on the Characteristics of Lonicera caerulea?Leaf by Segregation Method of Peroxyacetic Acid[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(03):80-83,105.[doi:10.11942/j.issn1002-2767.2016.03.0080]
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过氧乙酸离析法观测蓝靛果忍冬叶片脉序的方法研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2016年03 页码: 80-83,105 栏目: 出版日期: 2016-03-10
Title:
Observation on the Characteristics of Lonicera caerulea?Leaf by Segregation Method of Peroxyacetic Acid
文章编号:
1002-2767（2016）03-0080-04
作者:
徐清华1; 吴旭飞1; БРЫКСИН Д.М. 2; 张雪霞1; 李富恒1
1.东北农业大学 生命科学学院，黑龙江 哈尔滨 150030；2.俄罗斯米丘林园艺研究所，坦波夫州米丘林斯克市 393774
Author(s):
XU Qing-hua1; WU Xu-fei1; БРЫКСИН Д.М.2; ZHANG Xue-xia1; LI Fu-heng1
1.College of Life Sciences,Northeast Agricultural University,Harbin,Heilongjiang 150030; 2.The I.V.Michurin AllRussia Research Institute of Horticulture,Michurinsk,Tambov 393774
关键词:
蓝靛果忍冬; 叶片; 过氧乙酸离析法; 脉序; 正交试验
Keywords:
Lonicera caerulea;  leaves;  segregation method of peroxyacetic acid;  venation;  orthogonal design
分类号:
S663.9
DOI:
10.11942/j.issn1002-2767.2016.03.0080
文献标志码:
A
摘要:
过氧乙酸离析法主要用于植物叶片表皮的观测，也可用于叶片脉序的观测，操作简单实用。为获得适于蓝靛果忍冬叶片脉序观测的最佳组合，以蓝靛果忍冬叶片为材料，采用单因素试验和正交试验对过氧乙酸浓度、水浴温度、水浴时间的处理条件进行了系统的研究。结果表明：在70℃水浴中用12%的过氧乙酸处理蓝靛果忍冬叶片3.5 h或用14%的过氧乙酸溶液在60℃水浴中处理3.5 h，得到的装片脉序观测效果最佳。
Abstract:
Segregation method of Peroxyacetic acid was used to observe the epidermis of plant leaves and used for observing leaf nervation,it was a simple and practica method.Taking Lonicera caerulea?L.var.edulis Turcz.ex Herd.f.edulis as material,single factor experiment and orthogonal experiment were designed for the treatment conditions of Peracetic acid concentration,water-bath temperature and water-bath time.Observing leaf nervation was used in order to obtain the optimal reaction condition. The results of orthogonal test showed that the best condition of observation effect was 12% peroxyacetic acid treatment by 3.5 h water bath（70℃）or 14% peroxyacetic acid treatment by 3.5 h water bath(60℃) for fresh leaves.

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备注/Memo

收稿日期：2015-12-16

基金项目：黑龙江省教育厅资助项目（12531013）
第一作者简介：徐清华（1989-），女，山东省菏泽市人，在读硕士，从事植物生理研究。E-mail: 1272636460@qq.com。
通讯作者：李富恒（1962-），男，博士，教授，从事植物生理学的教学和科研工作。E-mail：lifuheng1963@126.com。

更新日期/Last Update: 2017-02-14