DUAN Chuan-ling,LAN Jing,ZHANG Hong-kun.Analysis on Error in Determining Amylose Content of Rice by Spectrophotometry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(04):129-132，133.[doi:10.11942/j.issn1002-2767.2015.04.0129]
分光光度法测定大米直链淀粉含量的误差分析
- Title:
- Analysis on Error in Determining Amylose Content of Rice by Spectrophotometry
- 文章编号:
- 1002-2767（2015）04-0129-04
- Keywords:
- spectrophotometry; rice; amylase; error
- 分类号:
- TS212.7；O657.32
- 文献标志码:
- A
- 摘要:
- 为了更准确的测定直链淀粉含量，在现有国家标准的基础上，探讨了直链淀粉测定过程中脱脂次数、酸度、碘的添加量、显色时间对吸光值的影响。结果表明:碘的加入量和显色时间对试验误差影响较大。碘的加入量应准确控制在1.0±0.1 mL，大于1.1 mL吸光值偏高，小于0.9 mL吸光值偏低。实验过程中同批样品显色时间应控制在2 h以内，以减少实验误差。脱脂次数和酸度对测定误差影响较小。
- Abstract:
- In order to accurately measure amylose content,the effect of degreasing,acidity ,addition of iodine and chromogenic time on the absorbance value was investigated on the basis of the existing national standards.The results showed that addition of iodine and chromogenic time had a great impact on the experimental error.Iodine’s adding amount should be accurately controlled between 1.0±0.1 mL,greater than 1.1 mL resulted in higher absorbance value,less than 0.9 mL induced lower absorbance value .In order to reduce experimental error,chromogenic time for the same batch of samples should be controlled within 2 hours.Degreasing times and acidity had little effect on measuring error.
参考文献/References:
［1］张巧杰.直链淀粉检测装置性能分析研究[D].北京：中国农业大学，2005.
[2]袁建.杨晓蓉.王肇慈.稻米直链淀粉含量测定方法的研究[J].谷物化学与检测分析，2000（1）:38-41.
[3]宋瑞芳.姚天明.直链、支链淀粉与碘显色反应的研究[J].安徽农业科学，1987（4）:38-40.
[4]GB7648-1987《水稻玉米谷子籽粒直链淀粉测定法》[S].
[5]Juliano B O.A simplified assay for milled rice amylase[J].Cereal Science,1971,16:334-340,360.
[6]陈轶雄.何孙胃.周少榕.分光光度法测定大米直链淀粉含量的不确定度[J].广东化工，2013（24）:147-148.
[7]武汉大学.分析化学上册[M].5版.北京：高等教育出版社，2006：313-325.
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备注/Memo
收稿日期：2014-12-12
第一作者简介：段传玲（1987-），女，山东省临沂市人，在读硕士，从事食品工程研究。
通讯作者：张宏坤（1979-），男，黑龙江省海伦市人，硕士,副教授，从事有机与高分子材料合成研究。E-mail：zhanghongkun2000@163.com。