LI Donglin,WANG Wenli,DENG Lailong,et al.KEGG Enrichment Analysis of Differential Metabolites in Different Varieties of Saffron (Crocus sativus L.) Under Drought Stress in Soilless Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2025,(07):79-87.[doi:10.11942/j.issn1002-2767.2025.07.0079]
无土栽培干旱胁迫下番红花不同品种差异代谢物KEGG富集分析
- Title:
- KEGG Enrichment Analysis of Differential Metabolites in Different Varieties of Saffron (Crocus sativus L.) Under Drought Stress in Soilless Culture
- 文章编号:
- 13
- Keywords:
- soilless culture; drought stress; saffron(Crocus sativus L.); different varieties; metabolites; KEGG analysis
- 文献标志码:
- A
- 摘要:
- 为促进番红花品种改良和资源优化利用，通过对干旱胁迫下番红花不同品种代谢物分析，筛选其差异代谢物，探索代谢物在番红花不同品种间的差异和变化规律。番红花不同品种采用室内无土亏水栽培，基于UPLC-MS/MS与GC-MS双检测平台和自建数据库检测番红花样本代谢物，对进行差异比较的分组样品进行主成分分析（PCA）、正交偏最小二乘法判别分析（OPLS-DA），选取变量重要性投影VIP>1和差异倍数（FC）值Fold Change≥2或Fold Change≤0.5来筛选差异代谢物，利用京都基因和基因组百科全书（KEGG）数据库进行功能注释和富集分析。共检测到2 458个代谢物，酚酸类占全部代谢物比例最高，达13.22%，其次是黄酮类、萜类和脂质类。筛选出番红花不同品种差异显著代谢物数目325个，其中下调代谢物数目244个， 上调81个。PCA第一主成分（PC1）贡献率为63.49%。总代谢物中被KEGG注释到的代谢物489个、差异显著代谢物79个。Metabolic pathways代谢通路下的差异代谢物为61个，占被注释差异代谢物总数的比例为77.22%。进一步对差异代谢物进行KEGG分析,发现国产番红花整体代谢物含量高于与原产地品种。
- Abstract:
- In order to promote the improvement of saffron varieties and optimize the utilization of resources, the differences and variation patterns of metabolites among different varieties of saffron were explored by experimental analysis of metabolites of different varieties of saffron under water deficit environment and Screening for differential metabolism. Different varieties of saffron were cultivated indoors without soil or water deficit, metabolites in saffron samples were detected using UPLC-MS/MS and GC-MS dual detection platforms and self built databases, differential grouped samples were compared with Principal Component Analysis (PCA) and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) . Differential metabolites were screened on the base of variable importance projection(VIP>1) and difference fold change values (FC≥2 or FC≤0.5), and functional annotation and enrichment analysis of differential metabolites were studied using Kyoto Encyclopedia of Genes and Genomes(KEGG) database. A total of 2 458 metabolites were detected in the experimental platform, phenolic acids account for the highest proportion of all metabolites, reached 13.22%, followed by flavonoids, terpenoids, and lipids. The 325 significantly different metabolites were screened in different varieties of saffron, including 244 down-regulated metabolites and 81 up-regulated metabolites. The contribution rate of the first principal component (PC1) of PCA reached 63.49%. The 489 metabolites and 79 significantly different metabolites were annotated by KEGG among the total metabolites. There were 61 differentially metabolites in the metabolic pathways, accounting for 77.22% of the total annotated differentially metabolites. Further KEGG analysis of differential metabolites revealed that the overall metabolite content of domestically produced saffron was higher than that of the native variety.
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备注/Memo
收稿日期：2025-04-10 基金项目：安徽省高校自然科学重点项目（KJ2021A1347）；校级横向科研重大项目（2025FZYHXKYPT102）；校级专项（成果培育转化）重点项目（2022KYXM14）。 第一作者：李东林（1979-），男，硕士，教授，从事植物种质资源开发应用与生理生态学研究。E-mail:ldlcareer@126.com。