ZHANG Huan,CAO Shang,FAN Xinyu,et al.Identification of a Pathogen Causing Root Rot Disease in Sorghum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2025,(04):14-17.[doi:10.11942/j.issn1002-2767.2025.04.0014]
一株引起高粱根腐病的病原菌鉴定
- Title:
- Identification of a Pathogen Causing Root Rot Disease in Sorghum
- 文章编号:
- 3
- 文献标志码:
- A
- 摘要:
- 为明确一株分离得到的高粱根腐病的病原菌种类，采用蘸根接种法进行柯赫氏法则验证，并基于ITS和β-tubulin基因序列分析，同时结合形态学对该菌进行鉴定。结果表明，菌株ZY5菌落正面绒状，规则圆形，黄褐色；菌落背面深黄褐色至黑色；厚垣孢子单生或串生，8～17μm×10～12μm；分生孢子长卵圆形，无隔膜，大小2.0～3.0 μm×1.0～1.5μm。基于β-tubulin基因序列构建的系统发育树，与高粱附球菌（Epicoccum sorghinum）聚为一支，支持率为98%。根据形态特征与构建的系统发育树的结果，将菌株ZY5鉴定为高粱附球菌。高粱附球菌可以引起高粱根腐病为国内外首次报道。
- Abstract:
- In order to identify the pathogen species of a separated sorghum root rot, the dipping root inoculation method was used to verify Koch’s postulates, and the fungus was identified based on the analysis of ITS and β-tubulin gene sequences, combined with morphological characteristics. The results indicated that the colony of strain ZY5 was velvety on the front, regular and circular, with a yellow brown color. The reverse side of the colony was dark yellow brown to black. Chlamydospores were either solitary or in chains, and sized 8-17 μm×10-12 μm. Conidia were oval-shaped, aseptate, and sized 2.0-3.0 μm×1.0-1.5 μm. The phylogenetic tree constructed based on β-tubulin sequences grouped ZY5 closely with Epicoccum sorghinum, with a support rate of 98%. Based on morphological characteristics and the constructed phylogenetic tree, strain ZY5 was identified as Epicoccum sorghinum. This is the first report domestically and internationally of Epicoccum sorghinum causing sorghum root rot.
相似文献/References:
[1]马长山,王胜华,张浩然,等.88%异丙甲草胺乳油防除高粱田杂草田间药效评价[J].黑龙江农业科学,2014,(07):70.
MA Chang-shan,WANG Sheng-hua,ZHANG Hao ran,et al.Efficacy Evaluation of 88% Metolachlor EC on Sorghum Weed[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(04):70.
[2]郑 旭,赵秀梅,王连霞,等.25%阿维〖DK1〗·福〖DK1〗·莠锈悬浮种衣剂病害防效试验[J].黑龙江农业科学,2014,(05):54.
ZHENG Xu,ZHAO Xiu-mei,WANG Lian-xia,et al.Field Efficacy Trial of 25% Abamectin·Thiram·Carboxin FSC Against Soybean Disease[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(04):54.
[3]沈海军.黑龙江省高粱出苗不全的原因与对策[J].黑龙江农业科学,2012,(02):152.
SHEN Hai-jun.Reasons and Countermeasures of Sorghum Incomplete Emergence in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(04):152.
[4]邢慧清,黄赛楠,张云鹤,等.分子标记在高粱抗病虫基因定位上的研究进展[J].黑龙江农业科学,2012,(09):139.
XING Hui-qing,HUANG Sai-nan,ZHANG Yun-he,et al.Research Advances of Mapping the Sorghum’s Resistance Genes to Disease and Inset by Molecular Markers[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(04):139.
[5]刘显元.不同药剂拌种对大豆根腐病的防治效果[J].黑龙江农业科学,2011,(04):67.
LIU Xian-yuan.Effect of Seeds Dressed with Different Medicament onControlling Soybean Root Rot[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(04):67.
[6]曾玲玲,刘 峰,崔秀辉,等.几种杀菌剂防治绿豆根腐病田间药效试验分析[J].黑龙江农业科学,2011,(09):45.
ZENG Ling-ling,LIU Feng,CUI Xiu-hui,et al.Analysis on the Field Tests of Several Kinds of Fungicides to Prevent Root Rot in Mung Bean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(04):45.
[7]赵秀梅.35%精甲霜灵种子处理乳剂防治花生根腐病田间药效试验[J].黑龙江农业科学,2010,(03):52.
ZHAO Xiu-mei.Experiment of 35% Fine Emulsion of Metalaxyl Seed Treatment to Prevent Root Rot in Peanut[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(04):52.
[8]李鹤鹏,杨广益.高粱除草药剂的筛选[J].黑龙江农业科学,2010,(12):65.
LI He-peng,YANG Guang-yi.Preemergence and Postemergence Herbicides Screening in Sorghum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(04):65.
[9]焦少杰,王黎明,姜艳喜,等.黑龙江省高粱生产发展演变过程分析[J].黑龙江农业科学,2015,(05):138.[doi:10.11942/j.issn1002-2767.2015.05.0138]
JIAO Shao-jie,WANG Li-ming,JIANG Yan-xi,et al.Analysis on Evolution Process of Sorghum Production Development in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(04):138.[doi:10.11942/j.issn1002-2767.2015.05.0138]
[10]季志强,张 勤,赵共鹏,等.承德地区高粱发展现状与应对措施分析[J].黑龙江农业科学,2015,(06):133.[doi:10.11942/j.issn1002-2767.2015.06.0133]
JI Zhi-qiang,ZHANG Qin,ZHAO Gong-peng,et al.Current Status and Countermeasures of the Sorghum Production in Chengde Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(04):133.[doi:10.11942/j.issn1002-2767.2015.06.0133]
备注/Memo
收稿日期：2024-11-27