YANG Li,WANG Yang,LI Shuai,et al.Observation and Analysis of Pollen Morphology of 20 Ornamental Lily Varieties[J].HEILONGJIANG AGRICULTURAL SCIENCES,2025,(02):45-51.[doi:10.11942/j.issn1002-2767.2025.02.0045]
20个观赏百合品种花粉形态观察与分析
- Title:
- Observation and Analysis of Pollen Morphology of 20 Ornamental Lily Varieties
- 文章编号:
- 7
- 文献标志码:
- A
- 摘要:
- 为了解观赏百合不同品种花粉的形态多样性和亲缘关系，选取5个系列20种观赏百合品种样品的花粉为供试材料，采用扫描电镜观察其花粉显微结构，分析观赏百合花粉的结构特征和亲缘关系，探讨其分类学意义。并根据花粉极轴长度（P）、赤道轴长度（E）、萌发沟长度、萌发沟宽度、极轴长度/赤道轴长度（P/E）和花粉粒大小(P×E)指标对供试材料进行聚类分析。扫描电镜观察分析结果表明，（1）供试的观赏百合花粉属于大花粉或极大花粉，以等极和两侧对称形式存在。极轴长度范围在73.37～121.49 μm之间，赤道轴长度范围在33.62～58.33 μm之间，萌发沟长度范围在62.49～108.55 μm之间，萌发沟宽度范围在1.16～5.07 μm之间，花粉粒大小（极轴×赤道轴）范围在2 617.19～6 560.80 μm2之间，极轴长度/赤道轴长度(P/E)范围为1.76～2.63，花粉形状均为长球形或超长球形单粒花粉，萌发孔类型均为单萌发沟，极面观为椭圆形或长椭圆形，均为N1P3C3型。表面纹饰分为盘珠状和瘤状，占样品总数的比例分别为30%和70%。（2）基于对观赏百合的花粉形态指标测量数据的Q型聚类分析。在遗传距离为12.5，7.5和3.5时，供试材料分别被分为3，5和7种类群，其中，‘小火箭’均为独立1类。
- Abstract:
- In order to understand the morphological diversity and phylogenetic relationships of pollen from different ornamental lily varieties, this study selected pollen samples from 20 ornamental lily varieties in 5 series as the test materials, used scanning electron microscopy to observe the microstructure of its pollen, analyzed the structural characteristics and phylogenetic relationships of ornamental lily pollen, and exploring its taxonomic significance. Cluster analysis was conducted on the test materials based on indicators such as pollen polar axis length (P), equatorial axis length (E), germination ditch length, germination ditch width, polar axis length/equatorial axis length (P/E), and pollen particle size (P×E). The results of scanning electron microscopy observation and analysis indicated that, (1) The ornamental lily pollen tested belongs to the category of large or extremely large pollen, which existed in an equipolar and symmetrical form on both sides. The length range of the polar axis were 73.37-121.49 μm. The length of the equatorial axis of pollen was 33.62-58.33 μm. The length range of the germination ditch were 62.49 -108.55 μm, and the width range of the germination ditch were 1.16-5.07 μm. The size of pollen grains (Polar axis × Equatorial axis) ranged from 2 617.19 to 6 560.80 μm2, and the polar axis length/equatorial axis length (P/E) ranged from 1.76 to 2.63. The shape of the pollen were either long spherical or ultra long spherical, with single grain pollen. The type of germination pore were single germination groove, and the polar view were elliptical or long elliptical, all of which were N1P3C3 type. The surface decoration were divided into disc-shaped and nodular shapes, accounting for 30% and 70% of the total sample size, respectively. (2) Q-type clustering analysis based on measurement data of pollen morphology indicators of ornamental lilies. At genetic distances of 12.5, 7.5, and 3.5, the test materials were divided into 3, 5, and 7 species groups, respectively, with Small rockets all belonging to a independent category varity.
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[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(02):75.[doi:10.11942/j.issn1002-2767.2021.02.0075]
备注/Memo
收稿日期：2024-05-31