[1]闫晓娟,谷勇哲,胡国龙,等.大豆种子蛋白亚基及11S/7S随种子发育的变化规律[J].黑龙江农业科学,2024,(04):11-17.[doi:10.11942/j.issn1002-2767.2024.04.0011]
　YAN Xiaojuan,GU Yongzhe,HU Guolong,et al.Changes of Soybean Seed Protein Subunits and 11S/7S with Seed Development[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(04):11-17.[doi:10.11942/j.issn1002-2767.2024.04.0011]
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大豆种子蛋白亚基及11S/7S随种子发育的变化规律

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2024年04 页码: 11-17 栏目: 出版日期: 2024-04-10
Title:
Changes of Soybean Seed Protein Subunits and 11S/7S with Seed Development
文章编号:
2
作者:
闫晓娟1; 谷勇哲2; 胡国龙2; 张素青3; 李欣3; 邳植1; 张福顺1; 4
（1.黑龙江大学 现代农业与生态环境学院，黑龙江 哈尔滨 150080； 2.中国农业科学院 作物科学 研究所，北京 100081； 3.内蒙古自治区农牧业技术推广中心，内蒙古 呼和浩特 010010； 4.农业农村部甜菜品质监督检验测试中心，黑龙江 哈尔滨 150080）
Author(s):
YAN Xiaojuan1; GU Yongzhe2; HU Guolong2; ZHANG Suqing3; LI Xin3; PI Zhi1;  ZHANG Fushun1; 4
(1.School of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, China; 2.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3. Inner Mongolia Autonomous Region Agriculture and Animal Husbandry Techonology Extension Center,Hohhot 010010, China; 4.Sugar Beet Quality Supervision and Inspection Testing Center of the Ministry of Agriculture and Rural Affairs, Harbin 150080, China)
关键词:
大豆;  蛋白;  11S/7S;  蛋白亚基;  SDS-PAGE
Keywords:
soybeans;  protein;  11S/7S;  protein subunits;  SDS-PAGE
DOI:
10.11942/j.issn1002-2767.2024.04.0011
文献标志码:
A
摘要:
为促进大豆蛋白品质改良，采用SDS-PAGE凝胶电泳对4份不同大豆品种种子发育过程中的蛋白亚基变化规律进行了分析。结果表明，在种子发育过程中发现蛋白含量高的品种中吉601（44.82%）在S4时期β亚基积累明显高于其他蛋白含量低的品种；不同大豆品种的11S组分的亚基在各个时期的积累明显不同，11S/7S比值低的两个品种中吉601（1.059）和吉林小粒豆（1.022），其11S组分中的Basic亚基在S3-S9时期的含量低于其他亚基的含量，而11S/7S比值高的两个品种黑河23（1.324）和绥农28（1.401），其11S组分中的Basic亚基和Acidic亚基在S3-S9时期含量高于其他亚基的含量，推测不同品种的11S/7S比值高低与Basic亚基在种子发育过程中的积累相关。
Abstract:
In order to promote the improvement of soybean protein quality, understand the dynamic changes of subunits of 11S and 7S components during the development of soybean seeds. in this study, SDS-PAGE was used to study the changes of protein subunits in four different soybean varieties during seed development. During seed development, it was found that the accumulation of β subunits in the variety Zhongji 601 (44.82%) with high protein content in the S4 stage was significantly higher than that of other varieties with low protein content. The accumulation of subunits in the 11S component of different soybean varieties was significantly different at different stages. Among the two varieties with low 11S/7S ratios, Zhongji 601 (1.059) and Jilin xiaolidou had a lower content of Basic subunits in the 11S component during the S3-S9 period compared to other subunits, while the two varieties with high 11S/7S ratios, Heihe 23 (1.324) and Suinong 28 (1.401), had a higher content of Basic and Acid subunits in the 11S component during the S3-S9 period compared to other subunits, which indicated the ratio of 11S/7S in different varieties was related to the accumulation of Basic subunits during seed development.

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备注/Memo

收稿日期：2024-01-11

基金项目：国家重点研发计划-中国和乌拉圭联合实验室合作项目（2018YFE0116900）；国家重点研发计划战略性科技创新合作专项（2022YFE0203300）。
第一作者：闫晓娟（1997-），女，硕士研究生，从事大豆遗传育种研究。E-mail：320804483@qq.com。
通信作者：张福顺（1965-），男，硕士，副研究员，从事农产品质量安全监督与营养评价研究。E-mail：bill6141@163.com。

更新日期/Last Update: 2024-04-10