CHEN Zhaogui,XIANG Xuemei,LI Xitao,et al.Callus Induction and Differentiation Technology of Potato "Favorita"[J].HEILONGJIANG AGRICULTURAL SCIENCES,2023,(07):20-24.[doi:10.11942/j.issn1002-2767.2023.07.0020]
马铃薯“费乌瑞它”愈伤组织诱导及分化技术
- Title:
- Callus Induction and Differentiation Technology of Potato "Favorita"
- 文章编号:
- 4
- Keywords:
- potato; "Favorita" ; callus; differentiation
- 文献标志码:
- A
- 摘要:
- 为建立马铃薯植株的离体再生体系,以“费乌瑞它”为材料,开展了不同激素配比对试管苗的继代、愈伤组织诱导及分化的影响研究。结果表明,试管苗继代培养适宜的激素配比为6-BA 0.20 mg·L-1+NAA 0.50 mg·L-1+ZT 0.50 mg·L-1,生根率75%,平均苗高2.757 cm;茎段愈伤组织最佳诱导激素配比为NAA 1.50 mg·L-1+2, 4-D 1.00 mg·L-1+6-BA 0.50 mg·L-1,诱导率达到92.00%;使用相同的激素组合诱导茎段和叶片愈伤组织,诱导率分别为89.66%和71.44%;愈伤组织分化最优激素配比为6-BA 1.00 mg·L-1+GA3 1.00 mg·L-1+ZT 1.00 mg·L-1,分化率为33.33%。初步建立的马铃薯“费乌瑞它”植株的离体再生体系可用于后续马铃薯遗传转化及基因编辑研究。
- Abstract:
- In order to establish the ex vivo regeneration system of potato plants, the effects of different hormone ratios on the succession, callus induction and differentiation of test tube seedlings were studied using "Favorita" as the material. The results showed that the suitable hormone ratio for IVF seedling subculture was 6-BA 0.20 mg·L-1+NAA 0.50 mg·L-1+ZT 0.50 mg·L-1, rooting rate was 75%, and the average seedling height was 2.757 cm. The optimal induction hormone ratio of stem callus was NAA 1.50 mg·L-1+2, 4-D 1.00 mg·L-1+6-BA 0.50 mg·L-1, and the induction rate reached 92.00%. The same hormone combination was used to induce callus of stem segment and leaf, and the induction rates were 89.66% and 71.44%, respectively. The optimal hormone ratio for callus differentiation was 6-BA 1.00 mg·L-1+GA3 1.00 mg·L-1+ZT 1.00 mg·L-1, and the differentiation rate was 33.33%. The preliminary established in vitro regeneration system of potato "Favorita" plants can be used for subsequent potato genetic transformation and gene editing research.
参考文献/References:
[1]屈聪玲,贺榆婷,王瑞良,等.植物转基因技术的过去、现在和未来[J].山西农业科学,2017,45(8):1376-1380,1383.[2]叶明旺,李灿辉,龚明.基因组编辑技术在马铃薯精准分子育种中的应用及研究展望[J].生物技术通报,2020,36(3):9-17.[3]齐恩芳.马铃薯再生体系建立及农杆菌介导 AcInv反义基因转化[D].兰州:甘肃农业大学,2006. [4]杨明贺,朱旭,李楠,等.马铃薯茎段高频再生体系的建立[J].东北农业科学,2019,44(1):57-62.[5]闫婷婷,贺媛,高茜,等.马铃薯品种 Favorita 再生体系的优化[J].分子植物育种,2017,15(8):3057-3062.[6]冯璐.植物生长物质对新品种马铃薯试管苗生长及微型薯诱导的影响[D].太原:山西农业大学,2017.[7]彭昕琴,胡家金,霍妙娟.马铃薯品种“大西洋”高效再生体系的建立[J].湖南农业科学,2008(2):26-28.[8]李凤云,盛万民,于天峰,等.马铃薯不同品种茎段再生系统的筛选[J].中国农业通报,2005,21(8):99-113.[9]牛瑜琦,孙蕾,梅超,等.马铃薯晋薯16号愈伤组织再生体系的建立[J].中国种业,2021(2):70-73.[10]侯丁一,张之为,田再民,等.马铃薯不同品种块茎再生体系的建立和优化[J].中国农学通报,2018,34(16):22-28.[11]冯光惠,杜虎平,李夏隆,等.费乌瑞它马铃薯茎尖分化成苗的培养基筛选与病毒的RT-PCR检测[J].湖北农业科学,2014,53(20):4988-4991.[12]张冬梅,修志君,冯亚艳,等.马铃薯茎段和叶片再生体系建立[J].内蒙古民族大学学报(自然科学版),2021,36(5):402-408.[13]鲍红春,李小雷,王建平,等.马铃薯品种陇薯 5号茎段再生体系的建立[J].内蒙古农业科技,2014(2):31-33.[14]李娟,程智慧,张国裕.马铃薯叶片高效再生体系的建立[J].西北植物学报,2004,24(4):610-614.[15]方贯娜,庞淑敏.马铃薯愈伤组织再生体系的研究进展[J].中国马铃薯,2012(5):307-310.[16]江林娟,邹雪,黄雪丽,等.响应面法优化马铃薯茎段高效再生体系[J].浙江农业学报,2018,30(6):918-925.[17]蒲秀琴.3种青海省主栽马铃薯外植体的组织培养和植株再生[J].江苏农业科学,2014,42(4):52-54.[18]DONG T, CAO Y, JIANG C Z.Cysteine protease inhibitors reduce enzymatic browning of potato by lowering the accumulation of free amino acids[J].Journal of Agricultural and Food Chemistry,2020, 68(8):2467-2476.[19]MA Y R,WANG H Y,YAN H.Pre-cut NaCl solution treatment effectively inhibited the browning of fresh-cut potato by influencing polyphenol oxidase activity and several free amino acids contents[J].Postharvest Biology and Technology,2021,178:111543.
相似文献/References:
[1]罗丽环,孙彬,张静华.沼渣与化肥配施对马铃薯产量与品质的影响[J].黑龙江农业科学,2014,(07):55.
LUO Li huan,SUN Bin,ZHANG Jing hua.Effect of Combined Application of Chemical Fertilizer and Biogas Residue on the Yield and Quality of Potatoes[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):55.
[2]赵海红.马铃薯地上垄体栽培模式中药剂拌种筛选试验[J].黑龙江农业科学,2013,(02):51.
ZHAO Hai-hong.Screening Experiment on Seed-dressing of Potatoin Aboveground Ridge Cultivation Model[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):51.
[3]陈 富,杨 谋,马廷蕊,等.马铃薯试管苗分层立体栽培繁育脱毒微型种薯[J].黑龙江农业科学,2012,(06):27.
CHEN Fu,YANG Mou,MA Ting-rui,et al.Plantlets Layered Hierarchical Cultivation toBreed Free-Virus Potato Minituber[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):27.
[4]毛彦芝,刘玲玲,孟兆华,等.EM菌对连作马铃薯原原种生产网棚土壤及马铃薯产量的影响[J].黑龙江农业科学,2014,(03):41.
MAO Yan zhi,LIU Ling ling,MENG Zhao hua,et al.Effect of EM on the Soil of Net Shed of Continuous Potato Cultivation and Yield[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):41.
[5]张荣华,刘双利,许庆芬,等.八种药剂对马铃薯晚疫病的防效及经济效益分析[J].黑龙江农业科学,2014,(03):60.
ZHANG Rong hua,LIU Shuang li,XU Qing fen,et al.Control Effect and Economic Benefits Analysis of Eight Pesticides on Potato Late Blight[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):60.
[6]房 磊.马铃薯新品种(系)引种对比试验初报[J].黑龙江农业科学,2012,(03):19.
FANG Lei.Preliminary Report of Comparison Testof New Introduced Potato Varieties (Lines)[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):19.
[7]苏君伟,赵 艳,王海新.辽西风沙半干旱区发展马铃薯膜下滴灌的探讨[J].黑龙江农业科学,2012,(03):48.
SU Jun-wei,ZHAO Yan,WANG Hai-xin.Discussion on Dripping Irrigation under Film of Potato in the Sandy and Semi-drought Area of Western Liaoning[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):48.
[8]宿飞飞,马 纪,胡林双,等.植物源杀菌剂对马铃薯组织培养中青霉菌的抑菌效果[J].黑龙江农业科学,2012,(03):81.
XV Fei-fei,MA Ji,HU Lin-shuang,et al.Impact of Herbal Fungicide on Inhibiting Penicillium in Medium of Potato Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):81.
[9]杨帆,黄先顺,李勇,等.马铃薯脱毒苗在不同基质管理方式下生产原原种的产量及效益分析[J].黑龙江农业科学,2012,(05):25.
YANG Fan,HUANG Xian-shun,LI Yong,et al.Potato Virus-free Plantlets under Different Matrix Management toProduce Breeder Seeds and Its Economic Benefit Analysis[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):25.
[10]董爱书,胡 新,邵晓梅,等.药剂拌种的时间与方式对马铃薯生长和产量的影响[J].黑龙江农业科学,2012,(06):77.
DONG Ai-shu,HU Xin,SHAO Xiao-mei,et al.Effect of Different Time and Way of Seed Dressingon Potato Growth and Yield[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):77.
备注/Memo
收稿日期:2023-03-27