XU Ya-xin,YANG Li,ZHAO Qian,et al.Effects of Chitosan and Microbial Agents on Photosynthesis and Resistance Physiology of Loquat Seedlings[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(12):33-37.[doi:10.11942/j.issn1002-2767.2022.12.0033]
壳聚糖及微生物菌剂对枇杷幼苗光合及抗性生理的影响
- Title:
- Effects of Chitosan and Microbial Agents on Photosynthesis and Resistance Physiology of Loquat Seedlings
- Keywords:
- chitosan; microbial agents; loquat; physiological characteristics
- 文献标志码:
- A
- 摘要:
- 为促进枇杷幼苗的生长,提高其光合能力和抗性,本文以‘大五星’枇杷幼苗为材料,对其叶面喷施不同浓度(0,1,2,4和6 g·L-1)的壳聚糖和不同稀释倍数(0,50,100,150和200倍液)的微生物菌剂,研究了壳聚糖和微生物菌剂对枇杷幼苗光合及抗性生理的影响。结果表明,叶面喷施不同浓度的壳聚糖提高了枇杷幼苗的叶绿素含量、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性和过氧化氢酶(CAT)活性。当壳聚糖浓度为2 g·L-1时叶绿素a含量、叶绿素b含量、类胡萝卜素含量、POD活性、SOD活性和CAT活性达最高值,分别较对照增加了28.03%、25.66%、6.84%、49.25%、15.72%和83.75%。不同浓度的微生物菌剂处理均提高了叶绿素b、类胡萝卜素和可溶性蛋白含量,仅100倍液处理时提高了叶绿素a含量。微生物菌剂50和100倍液处理提高了抗氧化酶活性,150倍液处理仅提高了POD活性,200倍液处理较对照无显著变化。故微生物菌剂100倍液处理效果最佳,枇杷幼苗叶片的叶绿素a含量、叶绿素b含量、类胡萝卜素含量、POD活性、SOD活性、CAT活性和可溶性蛋白含量较对照分别提高了11.69%、25.00%、18.37%、86.11%、2986%、18.23%和17.87%。因此,2 g·L-1壳聚糖处理和微生物菌剂100倍液处理最有利于提高枇杷幼苗的光合能力和抗性。
- Abstract:
- In order to promote the growth of loquat seedlings and improve their photosynthetic capacity and resistance, ‘Dawuxing’ loquat seedlings were sprayed with different concentrations (0, 1, 2, 4 and 6 g·L-1) of chitosan and different dilution times (0, 100,150 and 200 times) of microbial agents to study the effects of chitosan and microbial agents on photosynthesis and resistance physiology of loquat seedlings. The results showed that foliar application of different concentrations of chitosan increased chlorophyll content, peroxidase (POD) activity, superoxide dismutase (SOD) activity and catalase (CAT) activity of loquat seedlings. When the chitosan concentration was 2 g·L-1, the content of chlorophyll a and chlorophyll b, carotenoid content, POD activity, SOD activity and CAT activity reached the highest value, increased by 28.03%, 25.66%, 684%, 49.25%, 1572% and 83.75% respectively compared with the control. The treatments with different concentrations of microbial agents increased the contents of chlorophyll b, carotenoids and soluble protein in the leaves of loquat seedlings, and the content of chlorophyll a was increased only when the concentration was 100 times. The treatment with 50 and 100 times of microbial agents increased the activity of antioxidant enzymes. When the concentration was 150 times, only POD activity was increased. When the concentration was 200 times, there was no significant change compared with the control. Therefore, when the concentration of microbial agent was 100 times, the effect was the best. The content of chlorophyll aand chlorophyll b, carotenoid content, POD activity, SOD activity, CAT activity and soluble protein content in the leaves of loquat seedlings increased by 11.69%, 2500%, 18.37%, 86.11%, 29.86%, 18.23% and 17.87%, respectively, compared with the control. Therefore, when the chitosan concentration was 2 g·L-1 and the microbial agent concentration was 100 times, it was most conducive to improving the photosynthetic capacity and resistance of loquat seedlings.
参考文献/References:
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备注/Memo
收稿日期:2022-10-15