[1]郝小雨.硝化/脲酶抑制剂在玉米上的增产、增效及提质效应研究进展[J].黑龙江农业科学,2022,(10):103-108,118.[doi:10.11942/j.issn1002-2767.2022.10.0103]
　HAO Xiao-yu.Research Progress on Yield Increase, Efficieny Increase and Quality Improvement of Nitrification/Urease Inhibitors in Maize[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(10):103-108,118.[doi:10.11942/j.issn1002-2767.2022.10.0103]
点击复制

硝化/脲酶抑制剂在玉米上的增产、增效及提质效应研究进展

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2022年10 页码: 103-108,118 栏目: 出版日期: 2022-10-10
Title:
Research Progress on Yield Increase, Efficieny Increase and Quality Improvement of Nitrification/Urease Inhibitors in Maize
作者:
郝小雨
黑龙江省黑土保护利用研究院/农业农村部黑土地保护与利用重点实验室，黑龙江 哈尔滨 150086
Author(s):
HAO Xiao-yu
Heilongjiang Academy of Black Soil Conservation and Utilization/Laboratory of Black Soil Protection and Utilization， Ministry of Agriculture and Rural Areas，Harbin 150086, China
关键词:
玉米; 硝化抑制剂; 脲酶抑制剂; 产量; 品质; 氮肥利用率
Keywords:
maize; nitrification inhibitor; urease inhibitor; production; quality; nitrogen use efficiency
DOI:
10.11942/j.issn1002-2767.2022.10.0103
文献标志码:
A
摘要:
为了提高玉米产量和氮肥利用率，采用硝化抑制剂和脲酶抑制剂是调控土壤氮素转化及阻控农田土壤氮素损失有效措施。通过文献分析，明确了常用硝化抑制剂Nitrapyrin (2-氯-6-三氯甲基吡啶)、DCD（双氰胺）、DMPP（3，4-二甲基吡唑磷酸盐）和脲酶抑制剂NBPT（N-丁基硫代磷酰三胺）的抑制机理，阐述了硝化/脲酶抑制剂与氮肥单独配施或组合配施对玉米产量、品质和氮肥利用率的作用效应。分析表明，硝化/脲酶抑制剂配合施用的协同增效作用显著，能够延长氮素释放周期，促进玉米氮素吸收，既可提高玉米产量和氮肥利用效率，又可改善玉米籽粒品质。未来建议针对不同生态类型和不同土壤类型区域，在施用方法、影响机制、创新工艺等方面加强研究。
Abstract:
In order to improve maize yield and nitrogen use efficiency， nitrification inhibitors and urease inhibitors are effective measures to regulate soil nitrogen transformation and control soil nitrogen loss in farmland. Through literature analysis， the inhibition mechanisms of commonly used nitrification inhibitors Nitrapyrin [2-chloro-6-(trichloromethyl) pyridine]， DCD (Dicyandiamide)， DMPP (3，4-dimethylpyrazole phosphate) and urease inhibitor NBPT [N- (n-butyl) thiophosphoric triamide] were summarized， and the effects of nitrification/urease inhibitor combined with nitrogen application alone or in combination on maize yield， quality and nitrogen use efficiency were expounded. The analysis showed that the synergistic effect of nitrification/urease inhibitor combined application was obvious， which could prolong the nitrogen release cycle and promote the nitrogen absorption of maize. It could not only improve maize yield and nitrogen use efficiency， but also improve maize grain quality. In the future， it is recommended to strengthen research on application methods， impact mechanisms， and innovative processes for different ecological types and different soil types.

参考文献/References:

［1］彭东，沈玮囡，宋树柏，等.肥料增效剂对夏玉米植物学性状、产量构成及土壤中NH+4-N和NO-3-N的动态影响[J].玉米科学，2018，26(2):110-117.

[2]梁勇.2022年6月世界农产品供需形势预测简报[J].世界农业，2022(7):124-129.
[3]巨晓棠，张翀.论合理施氮的原则和指标[J].土壤学报，2021，58(1):1-13.
[4]米国华，伍大利，陈延玲，等.东北玉米化肥减施增效技术途径探讨[J].中国农业科学，2018，51(14)：2758-2770.
[5]张苗苗，沈菊培，贺纪正，等.硝化抑制剂的微生物抑制机理及其应用[J].农业环境科学学报，2014，33(11):2077-2083.
[6]张文学，孙刚，何萍，等.双季稻田添加脲酶抑制剂NBPT氮肥的最高减量潜力研究[J].植物营养与肥料学报，2014，20(4)：821-830.
[7]郝小雨，周宝库，马星竹，等.氮肥管理措施对黑土玉米田温室气体排放的影响[J].中国环境科学，2015，35(11):3227-3238.
[8]ZHANG L M，HU H W，SHEN J P，et al.Ammonia-oxidizing archaea have more important role than ammonia-oxidizing bacteria in ammonia oxidation of strongly acidic soils[J].The ISME Journal，2012，6:1032-1045.
[9]AMBERGER A.Research on dicyandiamide as a nitrification inhibitor and future outlook[J].Communications in Soil Science and Plant Analysis，1989，20：1933-1955.[10]VANNELLI T，HOOPER A B.Oxidation of nitrapyrin to 6-chloropicolinic acid by the ammonia-oxiding bacterium nitrosomonas europaea[J].Applied and Environmental Microbiology，1992，58(7):2321-2325.
[11]VANNELLI T，HOOPER A B.Reductive dehalogenation of the trichloromethyl group of nitrapyrin by the ammonia-oxidizing bacterium Nitrosomonas europaea [J].Applied and Environmental Microbiology，1993，59(11)：3597-3601.
[12]武志杰，史云峰，陈利军.硝化抑制剂作用机理研究进展[J].土壤通报，2008，39(4)：962-970.
[13]DI H J，CAMERON K C，JUPEI S，et al.Ammonia-oxidizing bacteria and archaea grow under contrasting soil nitrogen conditions[J].FEMS Microbiology Ecology，2010，72：386-394.
[14]KLEINEIDAM K，KOMRLJ K，KUBLIK S，et al.Influence of the nitrification inhibitor 3，4-dimethylpyrazole phosphate(DMPP) on ammonia-oxidizing bacteria and archaea in rhizosphere and bulk soil[J].Chemosphere，2011，84(1)：182-186.
[15]FLORIO A，CLARK I M，HIRSCH P R，et al.Effects of the nitrification inhibitor 3，4-dimethylpyrazole phosphate (DMPP) on abundance and activity of ammonia oxidizers in soil[J].Biology and Fertility of Soils，2014，50：795-807.
[16]疏晴，王香琪，齐永波，等.尿素与缓释尿素配施添加DMPP对砂姜黑土氮素转化的影响[J].土壤，2021，53(5)：945-951.
[17]郝小雨，马星竹，陈苗苗，等.氮肥配施增效剂实现寒地水稻增产、提质与增效[J].水土保持学报，2019，33(4):175-179，307.
[18]宋涛，尹俊慧，胡兆平，等.脲酶/硝化抑制剂减少农田土壤氮素损失的作用特征[J].农业资源与环境学报，2021，38(4)：585-597.
[19]MANUNZA B，DEIANA S，PINTORE M，et al.The binding mechanism of urea，hydroxamic acid and N-(N-butyl)-phosphoric triamide to the urease active site.A comparative molecular dynamics study[J].Soil Biology and Biochemistry，1999，31(5):789-796.
[20]REN B Z，ZHANG J W，DONG S T，et al.Nitrapyrin improves grain yield and nitrogen use efficiency of summer maize waterlogged in the field[J].Agronomy Journal，2017，109(1)：185-192.
[21]BORZOUEI A，MANDER U，TEEMUSK A，et al.Effects of the nitrification inhibitor nitrapyrin and tillage practices on yield-scaled nitrous oxide emission from a maize field in Iran[J].Pedosphere，2021，31(2)：314-322.
[22]何浩，张宇彤，危常州，等.不同有机替代减肥方式对玉米生长及土壤肥力的影响[J].水土保持学报，2019，33(5)：281-287.
[23]WARREN H L，HUBER D M，TSAI C Y，et al.Effect of nitrapyrin and N fertilizer on yield and mineral composition of corn[J].Agronomy Journal，1980，72(5)：729-732.
[24]崔磊，李东坡，武志杰，等.不同硝化抑制剂对红壤氮素硝化作用及玉米产量和氮素利用率的影响[J].应用生态学报，2021，32(11)：3953-3960.
[25]RAZA S，CHEN Z J，AHMED M，et al.Dicyandiamide application improved nitrogen use efficiency and decreased nitrogen losses in wheat-maize crop rotation in Loess Plateau[J].Archives of Agronomy and Soil Science，2019，65(4)：450-464.
[26]宋以玲，贺明荣，张吉旺，等.硝化抑制剂型包膜肥料对玉米生理特性、产量、品质的影响[J].河北科技师范学院学报，2015，29(1):6-11，80.
[27]LIU C，WANG K，ZHENG X.Effects of nitrification inhibitors (DCD and DMPP) on nitrous oxide emission，crop yield and nitrogen uptake in a wheat-maize cropping system[J].Biogeosciences，2013，10(4)：2427-2437.
[28]CUI L，LI D P，WU Z J，et al.Effects of combined nitrification inhibitors on nitrogen transformation，maize yield and nitrogen uptake in two different soils[J].Communications in Soil Science and Plant Analysis，2022，53(8)：1039-1049.
[29]CHATTERJEE A，CATTANACH N，AWALE R，et al.Can we reduce rainfed maize (Zea mays L.) nitrogenous fertilizer application rate with addition of nitrapyrin? [J].Communications in Soil Science and Plant Analysis，2016，47(4):527-532.
[30]郝小雨，马星竹，高中超，等.氮肥管理措施对黑土春玉米产量及氮素利用的影响[J].玉米科学，2016，24(4):151-159.
[31]CHEN Z M，LI Y，XU Y H，et al.Spring thaw pulses decrease annual N2O emissions reductions by nitrification inhibitors from a seasonally frozen cropland[J].Geoderma，2021，403，115310.
[32]LUO Y，BENKE M B，HAO X.Nutrient uptake and leaching from soil amended with cattle manure and nitrapyrin[J].Communications in Soil Science and Plant Analysis，2017，48(12)：1438-1454.
[33]郭松，喻华，陈琨，等.稳定性尿素对川中丘陵区小麦/玉米轮作制度下作物产量及氮肥利用率的影响[J].西南农业学报，2018，31(5)：1006-1012.
[34]MIGLIORATI M D A，SCHEER C，GRACE P R，et al.Influence of different nitrogen rates and DMPP nitrification inhibitor on annual N2O emissions from a subtropical wheat-maize cropping system[J].Agriculture，Ecosystems & Environment，2014，186：33-43.
[35]DEZ-LPEZ J A，HERNAIZ-ALGARRA P，SNCHEZ M A，et al.Effect of a nitrification inhibitor (DMPP) on nitrate leaching and maize yield during two growing seasons[J].Spanish Journal of Agricultural Research，2008(2)：294-303.
[36]BLACKMER A M，SANCHEZ C A.Response of corn to nitrogen-15-labeled anhydrous ammonia with and without nitrapyrin in Iowa[J].Agronomy Journal，1988，80(1)：95-102.
[37]BURZACO J P，CIAMPITTI I A，VYN T J.Nitrapyrin impacts on maize yield and nitrogen use efficiency with spring-applied nitrogen：Field studies vs.meta-analysis comparison[J].Agronomy Journal，2014，106(2)：753-760.
[38]REEVES D W，TOUCHTON J T.Relative phytotoxicity of dicyandiamide and availability of its nitrogen to cotton，corn，and grain sorghum[J].Soil Science Society of America Journal，1986，50(5)：1353-1357.
[39]THAPA R，CHATTERJEE A.Wheat production，nitrogen transformation，and nitrogen losses as affected by nitrification and double inhibitors[J].Agronomy Journal，2017，109(5)：1825-1835.
[40]KAWAKAMI E M，OOSTERHUIS D M，SNIDER J L，et al.Physiological and yield responses of field-grown cotton to application of urea with the urease inhibitor NBPT and the nitrification inhibitor DCD[J].European journal of agronomy，2012，43：147-154.
[41]DELL C J，HAN K，BRYANT R B，et al.Nitrous oxide emissions with enhanced efficiency nitrogen fertilizers in a rainfed system[J].Agronomy Journal，2014，106(2)：723-731.
[42]FISK L M，MACCARONE L D，BARTON L，et al.Nitrapyrin decreased nitrification of nitrogen released from soil organic matter but not amoA gene abundance at high soil temperature[J].Soil Biology and Biochemistry，2015，88：214-223.
[43]HU Y，SCHRAML M，VON TUCHER S，et al.Influence of nitrification inhibitors on yields of arable crops：A meta-analysis of recent studies in Germany[J].International Journal of Plant Production，2014，8(1)：33-50.
[44]张忠庆，高强.硝化抑制剂2-氯-6-三氯甲基吡啶在农业中应用研究进展及其影响因素[J].中国土壤与肥料，2022，(4)：249-258.
[45]王甄烨，焉莉，蒋富琛，等.脲酶/硝化抑制剂配施氮肥对春玉米农田氨排放的影响[J].玉米科学，2022，30(1)：138-143.
[46]李春玲，李国山，于亦忠，等.减氮施肥及氮肥添加脲酶抑制剂对凉州灌区春玉米产量和氮肥利用的影响[J].干旱地区农业研究，2021，39(2)：31-36.
[47]周丽娜，金建新，李凤霞，等.尿素配施脲酶抑制剂对春玉米株高和生物量的影响[J].宁夏农林科技，2017，58(12)：53-55.
[48]CHIEN S H，PROCHNOW L I，CANTARELLA H.Recent developments of fertilizer production and use to improve nutrient efficiency and minimize environmental impacts[J].Advances in Agronomy，2009，102：267-322.
[49]TRENKEL M E.Controlled-release and stabilized fertilizers in agriculture[M].Paris：International Fertilizer Industry Association，1997.
[50]MUHAYMIN M Z M，NORAINI M J，ZAHARAH S S，et al.N-(n-Butyl) thiophosphoric triamide (NBPT)-coated urea (NCU) improved maize growth and nitrogen use efficiency (NUE) in highly weathered tropical soil[J].Sustainability，2020，12(21)：8780.
[51]MARTINS M R，SANT’ANNA S A C，ZAMAN M，et al.Strategies for the use of urease and nitrification inhibitors with urea：Impact on N2O and NH3 emissions，fertilizer-15N recovery and maize yield in a tropical soil[J].Agriculture，Ecosystems & Environment，2017，247：54-62.
[52]GOU W，ZHENG P F，TIAN L，et al.Exogenous application of urea and a urease inhibitor improves drought stress tolerance in maize (Zea mays L.)[J].Journal of Plant Research，2017，130(3)：599-609.
[53]TURNER D A，EDIS R B，CHEN D，et al.Determination and mitigation of ammonia loss from urea applied to winter wheat with N-(n-butyl) thiophosphorictriamide[J].Agriculture，Ecosystems & Environment，2010，137(3)：261-266.
[54]DEMPSEY R J，SLATON N A，ROBERTS T L，et al.Rice grain yield and nitrogen uptake as affected by urea amendment and rainfall timing[J].Agronomy Journal，2017，109(6)：2966-2973.
[55]ZHANG L，WU Z，JIANG Y，et al.Fate of applied urea15 N in a soil-maize system as affected by urease inhibitor and nitrification inhibitor[J].Plant，Soil and Environment，2010，56(1)：8-15.
[56]LUCAS F T，BORGES B M M N，COUTINHO E L M.Nitrogen fertilizer management for maize production under tropical climate[J].Agronomy Journal，2019，111(4)：2031-2037.
[57]BESEN M R，RIBEIRO R H，MINATO E A，et al.Modelling of N2O emissions from a maize crop after the application of enhanced-efficiency nitrogen fertilisers[J].Communications in Soil Science and Plant Analysis，2021，52(14)：1645-1656.
[58]CANCELLIER E L，SILVA D R G，FAQUIN V，et al.Ammonia volatilization from enhanced-efficiency urea on no-till maize in brazilian cerrado with improved soil fertility[J].Ciência E Agrotecnologia，2016，40：133-144.
[59]LIU G Y，YANG Z P，DU J，et al.Adding NBPT to urea increases N use efficiency of maize and decreases the abundance of N-cycling soil microbes under reduced fertilizer-N rate on the North China Plain[J].PloS One，2020，15(10)：e0240925.
[60]AMDADUL H M，ZAHARAH S S，PHEBE D，et al.Foliar urea with N-(n-butyl) thiophosphoric triamide for sustainable yield and quality of pineapple in a controlled environment[J].Sustainability，2021，13(12)：6880.
[61]FAN D J，HE W T，SMITH W N，et al.Global evaluation of inhibitor impacts on ammonia and nitrous oxide emissions from agricultural soils：A meta-analysis[J].Global Change Biology,2022，28(17)：5121-5141
[62]武志杰，石元亮，李东坡，等.稳定性肥料发展与展望[J].植物营养与肥料学报，2017，23(6):1614-1621.
[63]ZAMAN M，ZAMAN S，ADHINARAYANAN C，et al.Effects of urease and nitrification inhibitors on the efficient use of urea for pastoral systems[J].Soil Science and Plant Nutrition，2013，59(4):649-659.
[64]柯福来，黄瑞冬，马兴林，等.化学抑制型氮素释放延缓剂对玉米产量和品质的影响[J].玉米科学，2007,15(2)：111-113.
[65]王玲莉，古慧娟，石元亮，等.尿素配施添加剂NAM对三江平原白浆土氮素转化和玉米产量的影响[J].中国土壤与肥料，2012(2):34-38.
[66]孙磊.NAM肥料添加剂在寒地玉米上的应用效果[J].黑龙江农业科学，2017(7):22-24.
[67]李学红，李东坡，武志杰，等.添加NBPT/DMPP/CP的高效稳定性尿素在黑土和褐土中的施用效应[J].植物营养与肥料学报，2021，27(6):957-968.
[68]张蕾，王玲莉，房娜娜，等.稳定性肥料在中国不同区域的施用效果及施用量[J].植物营养与肥料学报，2021，27(2)：215-230.

相似文献/References:

[1]王静.西昌地区玉米自交系苗期耐低磷特性鉴定[J].黑龙江农业科学,2013,(02):5.
　WANG Jing.Identification of Resistance to Low-phosphorus Characteristics of Maize Inbred Lines at Seedling Stage in Xichang Area[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(10):5.
[2]石凤梅,马立功.黑龙江省玉米大斑病菌小种生理分化的研究[J].黑龙江农业科学,2013,(02):63.
　SHI Feng-mei,MA Li-gong.Study on Physiological Race Differentiation of Exserohilum turcicum in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(10):63.
[3]孙广全,焦少杰,王黎明,等.乌克兰玉米自交系芽期耐旱性鉴定[J].黑龙江农业科学,2014,(11):4.
　SUN Guang-quan,JIAO Shao-jie,WANG Li-ming,et al.Drought Tolerance Identification of Ukrainian Maize Inbred Lines at Bud Stage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(10):4.
[4]马延华,孙德全,李绥艳,等.玉米自交系灌浆期抗倒性评价及抗倒性状的相关分析[J].黑龙江农业科学,2014,(07):5.
　MA Yan-hua,SUN De-quan,LI Sui-yan,et al.Evaluation of Lodging Resistance and Correlation Analysis of Lodging Resistance Characters for Maizenbred Lines During Grain filling Period[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(10):5.
[5]杨耿斌,刘兴焱,王立春,等.黑龙江早熟地区深松对玉米产量及土壤状态的影响[J].黑龙江农业科学,2014,(07):23.
　YANG Geng-bin,LIU Xing-yan,WANG Li-chun,et al.Effect of Subsoiling on Maize Yield and Soil Characteristics in Earlymaturing District of Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(10):23.
[6]王宇先.寒地半干旱地区膜上精播技术对玉米生长发育的影响[J].黑龙江农业科学,2014,(07):29.
　WANG Yu-xian.Effect of Planting in Mulching Film on the Growth and Development of Maize Plant in Cold and Semi Arid Area[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(10):29.
[7]刘志华,盖兆雪,李晓梅,等.秸秆还田对玉米产量形成及土壤肥力的影响[J].黑龙江农业科学,2014,(07):42.
　LIU Zhi-hua,GAI Zhao-xue,LI Xiao-mei,et al.Effect of Straw Return on Maize Yield Components and Soil Fertility[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(10):42.
[8]林红,何晶丽.玉米在畜牧养殖业中的有效利用[J].黑龙江农业科学,2014,(07):77.
　LIN Hong,HE Jing-li.Effective Utilization of Maize in Animal Husbandry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(10):77.
[9]林 红,孙德全,李绥艳,等.玉米单倍体诱导系对不同类群F1的诱导率评价[J].黑龙江农业科学,2014,(06):12.
[10]张崎峰,蔡鑫鑫,李金良,等.黑龙江省高纬寒地大垄双行栽培模式下玉米品种筛选试验[J].黑龙江农业科学,2014,(06):22.
　ZHANG Qifeng,CAI Xinxin,LI Jinliang,et al.Screening Test of Maize Varieties under the Mode of Double Row Sowing with Big Ridge in High Latitude and Cold Region of Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(10):22.
[11]孙磊.NAM肥料添加剂在寒地玉米上的应用效果[J].黑龙江农业科学,2017,(07):22.[doi:10.11942/j.issn1002-2767.2017.07.0022]
　SUN Lei.Effect of NAM Fertilizer Additive on Maize in Cold Area[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(10):22.[doi:10.11942/j.issn1002-2767.2017.07.0022]

备注/Memo

收稿日期：2022-07-29

基金项目：省级黑土地保护利用-典型地区耕地监测评价项目（2022-22-2）；科技基础资源调查专项（2021FY100404-1）；黑龙江省农业科学院“农业科技创新跨越工程”专项(HNK2019CX1310)。
作者简介：郝小雨（1981－），男，博士，副研究员，从事农田养分循环研究。E-mail：xiaoyuhao1981@sina.com。

更新日期/Last Update: 2022-12-26