[1]南芝润,侯磊,张杰,等.玉米自交系中氨基酸含量与品质性状相关分析[J].黑龙江农业科学,2022,(08):96-100,110.[doi:10.11942/j.issn1002-2767.2022.08.0096]
　NAN Zhi-run,HOU Lei,ZHANG Jie,et al.Correlation Analysis of Amino Acid Content and Quality Traits in Maize Inbred Lines[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(08):96-100,110.[doi:10.11942/j.issn1002-2767.2022.08.0096]
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玉米自交系中氨基酸含量与品质性状相关分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2022年08 页码: 96-100,110 栏目: 出版日期: 2022-08-10
Title:
Correlation Analysis of Amino Acid Content and Quality Traits in Maize Inbred Lines
作者:
南芝润; 侯磊; 张杰; 田怀泽
山西农业大学 玉米研究所，山西 忻州 034000
Author(s):
NAN Zhi-run; HOU Lei; ZHANG Jie; TIAN Huai-ze
Maize Research Institute,Shanxi Agricultural University,Xinzhou 034000,China
关键词:
玉米自交系; 丝氨酸; 氨基酸
Keywords:
maize inbred line;  serine;  amino acid
DOI:
10.11942/j.issn1002-2767.2022.08.0096
文献标志码:
A
摘要:
为了研究丝氨酸含量不同的自交系中氨基酸的组成及含量，从而对玉米种质资源的营养品质进行评价，以从500份玉米优良自交系中选取的丝氨酸含量和农艺性状差异较大的20份自交系作为研究对象，利用近红外分析仪测定玉米自交系籽粒的水分、蛋白质、淀粉、脂肪、粗纤维和氨基酸含量，并对其品质性状和游离氨基酸含量分别进行相关分析。结果表明，总氨基酸含量与蛋白质含量呈极显著正相关，与水分含量、淀粉含量呈极显著负相关。丝氨酸含量与蛋白质含量、赖氨酸含量、脯氨酸含量都呈极显著正相关。游离丝氨酸含量与限制性氨基酸蛋氨酸、赖氨酸呈显著或极显著相关。说明在提高丝氨酸含量的同时蛋白含量和赖氨酸含量也会显著提高。
Abstract:
In order to analyze the components and content of amino acids in inbred lines with different serine contents and evaluate the nutritional quality of maize germplasm resources.20 inbred lines of fine serine content and agronomic traits differences were selected from 500 maize inbred lines as the research object.Moisture,protein,starch,fat,crude fiber and amino acids of maize inbred lines were determined by near infrared analyzer.Free amino acid content were measured by amino acid analyzer.The quality characters and contents of free amino acids were analyzed.The results showed that the total amino acid content was significantly positively correlated with protein content,and negatively correlated with water content and starch content.Serine content was significantly positively correlated with protein content,lysine content and proline content.The content of free serine was significantly correlated with the limiting amino acids methionine and lysine.In conclusion while increasing the content of serine,the content of protein and lysine were also significantly increased.

参考文献/References:

［1］于玲玲,李俊鹏,甄志华,等.青贮玉米品质影响因素分析[J].中国饲料,2021（21）:127-129.

[2]侯俊峰,韩海亮,陈斌,等.甜玉米和糯玉米品质特征和育种相关研究概述[J].农业科技通讯,2021（12）:9-13.

[3]李坤,李高科,肖颖妮,等.甜玉米品质遗传改良研究进展[J].广东农业科学,2020,47(11)：70-77.

[4]刘治先,ALLEN D W.玉米胚乳蛋白质及氨基酸遗传控制的研究现状[J].山东农业科学,1996(1):8-13.

[5]于泓,牟世芬.氨基酸分析方法的研究进展[J].分析化学,2005（3）:398-404.

[6]NODA H,AMANO K.Sugars,organic acids and minerals of nor,the dried laver porphyra[J].Bulletin of the Chemical Society of Japan,1981,47:57-62.

[7]李梅,刘金华,楼兵干,等.植物鲜样中游离氨基酸提取方法的比较[J].实验室研究与探索,2016,35(4):34-38,51.

[8]马瑞肖,张慧杰,李馨慧,等.丝氨酸/甘氨酸代谢在肿瘤中的研究进展[J].现代肿瘤医学，2020,28(6):1021-1024.

[9]孙其伶,肖辉,郑倩.磷脂酰丝氨酸外翻在细胞凋亡及其清除过程中的分子调控机制[J].生命的化学，2020,40(8):1199-1207.

[10]HO C L,SAITO K.Molecular biology of the plastidic phosphorylated serine biosynthetic pathway in Arabidopsis thaliana[J].Amino Acids,2001,20:243-259.

[11]KISHOR P B K,SURAVAJHALA R,MARKA N,et al.Lysine,lysine-rich,serine,and serine-rich proteins:link between metabolism,development,and abiotic stress tolerance and plants[J].Frontiers in Plant Science,2020,11：1-15.

[12]徐春英.冬小麦氨基酸代谢与抗旱性关系的研究[D].北京：中国农业科学院,2008.

[13]王建革,张丽荣,王利民,等.中国高赖氨酸玉米育种终结[J].中国科技史料,1996,20(4):352-357.

[14]ROS R,MUNOZ-BERTOMEU J,KRUEGER S.Serine in plants:Biosynthesis,metabolism,and functions[J].Trends in Plant Science,2014,19:564-569.

[15]MATTAINI K R,SULLIVAN M R,HEIDEN M G V.The importance of serine metabolism in cancer[J].Cell Biology,2016,214:249-257.

[16]MICHARD E,LIMA P T,BORGES F,et al.Glutamate receptor-like genes form Ca 2+ channels in pollen tubes and are regulated by pistil D-serine[J].Science,2011,332:434-437. [17]CASCALES-MINANA B,MUNOZ-BERTOMEU J,FLORES-TORNERO M,et al.The phosphorylated pathway of serine biosynthesis is essential both for male gametophyte and embryo development and for root growth in Arabidopsis[J].Plant Cell,2013,25:2084-2101.

[18]MUOZ-BERTOMEU J,CASCALES-MIANA B,MULET J M,et al.Plastidial glyceraldehyde-3-phosphate dehydrogenase deficiency leads to altered root development and affects the sugar and amino acid balance in Arabidopsis[J].Plant Physiology,2009,151:541-558.

[19]刘荣森,杨虹琦,黄郁维,等.植物中游离氨基酸的提取、纯化及分析方法[J].河南科技大学学报,2007,28(3):76-79.

[20]SHARMA M K,FANTA S.Variance balanced designs for complete diallel cross[J].Journal of Agricultural Biotechnology and Sustainable Development,2010,24:56-60.

[21]BAHARI M,RAFII M Y,SALEH G B,et al.Combining ability analysis in complete diallel cross of watermelon(Citrullus lanatus(Thunb.) Matsum.＆Nakai)[J].The Scientific World Journa1,2012,2012:543158. [22]MOTERLE L M,DE LUCA E,BRACCINI A,et al.Combining ability of popcorn lines for seed quality and agronomic traits[J].Euphytica,2012,185(3):337-347.

[23]ZHAO X Y,BIAN X Y,LIU M R,et al.Analysis of genetic effects on a complete diallel cross test of Betula platyphylla[J].Euphytica,2014,205:221-229.

[24]孟强,王薪淇,宋轶群,等.糯玉米自交系中游离氨基酸的组成及含量分析[J].西北农林科技大学学报,2015,43(3):91-97,104.

[25]孙志东,孙晓秋,赵桐,等.糯玉米游离氨基酸含量与品质性状相关分析[J/OL].吉林农业大学学报,2021:1-10[2022-0624].http://kns.cnki.net/kcms/detail/22.1100.S.20210421.1105.004.

[26]祁新,赵颖君,梁军,等.玉米氨基酸含量的杂种优势分析[J].吉林农业大学学报,2001,23(2):17-20.

 

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备注/Memo

收稿日期：2022-05-20

基金项目：山西省农业科学院农业科技创新研究课题（YCX2020412）；山西省重点研发计划项目（201803D221005-4）；山西省农业科学院生物育种工程项目（17yzgc065）；山西农业大学玉米研究所科研项目（yms2021-07）。

第一作者：南芝润（1979-)，女，硕士，副研究员，从事作物育种与农产品加工研究。E-mail：yexue139@126.com。

更新日期/Last Update: 2022-10-09