[1]符堯,李静,张子安,等.一株抗大豆胞囊线虫芽孢杆菌的分离鉴定[J].黑龙江农业科学,2022,(02):36-40.[doi:DOI:10.11942/j.issn1002-2767.2022.02.0036]
　FU Yao,LI Jing,ZHANG Zi-an,et al.Separation and Identification of Bacillus sp.Antagonistic to Soybean Cyst Nematode[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(02):36-40.[doi:DOI:10.11942/j.issn1002-2767.2022.02.0036]
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一株抗大豆胞囊线虫芽孢杆菌的分离鉴定

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2022年02 页码: 36-40 栏目: 出版日期: 2022-02-10
Title:
Separation and Identification of Bacillus sp.Antagonistic to Soybean Cyst Nematode
作者:
符堯1; 李静1; 张子安1; 周园园1; 2
1.黑龙江八一农垦大学 农学院，黑龙江 大庆 163316；2.国家杂粮工程技术研究中心，黑龙江 大庆 163319
Author(s):
FU Yao1; LI Jing1; ZHANG Zi-an1; ZHOU Yuan-yuan1; 2
1.Agricultural College,Heilongjiang Bayi Agricultural University,Daqing 163316，China；2.National Coarse Cereals Engineering Research Center，Daqing 163319，China
关键词:
大豆胞囊线虫; 芽孢杆菌; 分离; 鉴定
Keywords:
Heterodera glycines;  Bacillus;  seperation;  identification
DOI:
DOI:10.11942/j.issn1002-2767.2022.02.0036
文献标志码:
A
摘要:
为了筛选用作防治大豆胞囊线虫的微生态制剂的益生菌，从大豆根围土中分离芽孢杆菌Bal-7，并测定其发酵液对大豆胞囊线虫2龄幼虫活性的影响。结合形态学、生理生化特征及16S rRNA基因序列比对分析对菌株进行鉴定。结果表明：分离筛选的芽孢杆菌Ba1-7发酵液对大豆胞囊线虫2龄幼虫校正死亡率为83.32％，经分子生物学鉴定菌株Ba1-7为阿氏芽孢杆菌（Bacillus aryabhattai）。菌株Ba1-7可用作防治大豆胞囊线虫的生防菌剂。 
Abstract:
In order to screen biocontrol strains against soybean cyst nematode,Bacillus Ba1-7 was isolated from soybean rhizosphere soil,identified by morphological,physiological and biochemical characteristics and 16S rRNA gene sequence alignment analysis.The inhibition rate of the fermentation of the strain Ba1-7 on soybean cyst nematode were determined.The results showed that the isolated and screened Ba1-7 was Bacillus aryabhattai.The correted mortality rate of the Ba1-7 fermentation on the hatching of J2 was 83.32%.Strain Ba1-7 can be used as a biocontrol agent against soybean cyst nematode. 

参考文献/References:

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备注/Memo

收稿日期：2021-11-13

基金项目：黑龙江省大学生创新创业训练计划(202010223105)；大庆市指导性科技计划（zd-2021-72）。

第一作者：符堯（1999-），女，本科生，专业为植物保护。E-mail：1067603370@qq.com。

通信作者：周园园（1988-），女，博士，讲师，从事植物保护相关研究。E-mail：zhouyuanyuan6616@163.com。

更新日期/Last Update: 2022-04-02