[1]刘海英,杨忠良,刘会,等.五优稻4号水稻香味的遗传分析与SSR分子标记筛选[J].黑龙江农业科学,2021,(06):5-9.[doi:10.11942/j.issn1002-2767.2021.06.0005]
　LIU Hai-ying,YANG Zhong-liang,LIU Hui,et al.Genetic Analysis and SSR Molecular Marker Screening for Fragrance Gene in Rice Wuyoudao 4[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(06):5-9.[doi:10.11942/j.issn1002-2767.2021.06.0005]
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五优稻4号水稻香味的遗传分析与SSR分子标记筛选

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2021年06 页码: 5-9 栏目: 出版日期: 2021-06-10
Title:
Genetic Analysis and SSR Molecular Marker Screening for Fragrance Gene in Rice Wuyoudao 4
作者:
刘海英1; 2; 杨忠良2; 刘会2; 冷春旭2; 吴立成2; 徐振华2; 于艳敏2; 来永才3
1.黑龙江省农业科学院 博士后科研工作站，黑龙江 哈尔滨 150086；2.黑龙江省农业科学院 生物技术研究所，黑龙江 哈尔滨 150028;3.黑龙江省农业科学院，黑龙江 哈尔滨 150086
Author(s):
LIU Hai-ying1; 2; YANG Zhong-liang2; LIU Hui2; LENG Cun-xu2; WU Li-cheng2; XU Zhen-hua2; YU Yan-min2; LAI Yong-cai1
1.Postdoctoral Programme,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China; 2.Institute of Biotechnology,Heilongjiang Academy of Agricultural Sciences,Harbin 150028,China;3.Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China
关键词:
水稻; 香味; 遗传分析; SSR分子标记
Keywords:
rice;  fragrance genetic analysis;  SSR molecular marker
DOI:
10.11942/j.issn1002-2767.2021.06.0005
文献标志码:
A
摘要:
为促进水稻香味分子标记辅助育种，以优质香稻品种五优稻4号为香味供体亲本，龙粳20为受体亲本进行杂交，根据杂交后代植株香味的表现，对五优稻4号水稻品种香味性状进行遗传分析，并利用SSR分子标记方法筛选与五优稻4号香味连锁的SSR分子标记。结果表明：水稻五优稻4号的香味性状是由单隐性基因控制；通过SSR分子标记引物的筛选，得到了49对能够在两亲本间稳定表现出多态性的引物，用这49对引物筛选由杂交后代构建的选香和无香基因池，得到1个SSR引物RM5647与香味基因紧密连锁，其遗传距离为25.7 cM。
Abstract:
In order to promote molecular marker assisted breeding of rice aroma,in this paper,F1 and F2 segreation population derived from a cross between fragrant rice variety Wuyoudao 4 and non-fragrant rice variety Longjing 20 were used to study the inheritance of fragrance trait and initial mapping the fragrance gene by SSR molecular marks in rice.The results showed that the fragrance trait of rice variety Wuyoudao 4 was controlled by one recessive gene and fragrance was recessive and non-fragrance was dominant.Through primer screening,forty-nine pairs of primers showing steady polymorphism in parents were selected.By screening the fragrance and non-fragrance bulked using these primers,one SSR markers RM5647 linked distance of 25.7 cM.

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备注/Memo

收稿日期：2021-01-30

基金项目：黑龙江省自然科学基金面上项目（C2015029）；国家重点研发计划(2016YFD0300900)；黑龙江省科技厅重点研发项目（GA18B101）。
第一作者：刘海英（1978-），女，博士，助理研究员，从事水稻育种栽培研究。E-mail:liuhaiying0906@126.com。

更新日期/Last Update: 2021-07-21