HAO Shi-xuan,QI Hong-ying,ZHANG Quan,et al.Study on Callus Induction of Watermelon[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(09):25-27.[doi:10.11942/j.issn1002-2767.2019.09.0025]
西瓜愈伤组织诱导研究
- Title:
- Study on Callus Induction of Watermelon
- 文献标志码:
- A
- 摘要:
- 为促进西瓜离体快繁和工厂化育苗,以西瓜无菌苗为试验材料,研究影响西瓜愈伤组织形成的条件因素。结果表明:在25 ℃暗培养条件下,西瓜子叶节最适合进行愈伤组织诱导培养,西瓜子叶节外植体愈伤组织诱导率最高,为87.55%。7 d龄期西瓜无菌苗子叶节诱导出的愈伤组织,诱导率最高,达到89.62%,状态良好。西瓜子叶节诱导愈伤组织适宜激素浓度配比为2.0 mg·L-1- 2,4-D+1.0 mg·L-1- 6-BA。因此,西瓜愈伤组织形成的最佳条件为选用7 d龄期西瓜无菌苗子叶节为外植体,在MS+2.0 mg·L-1- 2,4-D+1.0 mg·L-1- 6-BA-的培养基上进行诱导。
参考文献/References:
[1]-FAOSTAT.Food and Agriculture Organization Corporate Statistical Database[EB/OL].[2019-04-01].http://www.fao.org/.
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备注/Memo
收稿日期:2019-04-01