WANG Miao-miao,HAN Fei,YAN Huan,et al.Inhibitory Effect of Lavandula angustifolia Mill. Extract on Acetylcholin Esterase[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(08):124-127.[doi:10.11942/j.issn1002-2767.2019.08.0124]
薰衣草提取物对乙酰胆碱酯酶的抑制作用
- Title:
- Inhibitory Effect of Lavandula angustifolia Mill. Extract on Acetylcholin Esterase
- Keywords:
- Lavandula angustifolia Mill. extract; acetylcholin esterase; inhibitor; Alzheimer’s disease; enzyme-labeling instrument
- 文献标志码:
- A
- 摘要:
- 为从薰衣草提取物中寻找潜在的治疗阿尔茨海默病的乙酰胆碱酯酶抑制剂。采用经乙醇提取，聚酰胺柱、HP20树脂柱及制备液相分离纯化后，得到30个薰衣草提取物，测定其多酚含量，并以石杉碱甲为阳性对照，采用酶标仪测定405 nm 处吸光度值以筛选其乙酰胆碱酯酶抑制活性并计算抑制率，分析二者相关性。结果表明：薰衣草提取物对乙酰胆碱酯酶表现出了不同的抑制作用（抑制率3.00%~82.78%），其中7个提取物作用较为显著，抑制率均大于50%；有4个提取物表现出了微弱的抑制作用（抑制率小于10%）；另外，近一半的薰衣草提取物多酚含量大于20 μg·mL-1。薰衣草提取物具有乙酰胆碱酯酶抑制活性，表明其含有抑制乙酰胆碱酯酶的成分，经过相关性分析，多酚可能不是起主要作用的活性物质，为进一步跟踪分离活性成分奠定基础。
- Abstract:
- In order to find potential inhibitors from Lavandula angustifolia Mill.extract for acetylcholin esterase that can be used in the treatment of Alzheimer′s disease.Lavandula angustifolia Mill.was extracted by ethanol and purified by polyamide column,HP20 resin column and preparative liquid chromatography.The Folin-phenol reagent method was used for the determination of total phenolics Lavandula angustifolia Mill.Extract Totally 30 extracts from Lavandula angustifolia Mill.were served as inhibitors,Huperzine A was used as the positive control to detect the absorbance value at 405 nm in vitro enzymatic reaction system by enzyme-labeling instrument.The inhibitory activity on the extracts was calculated.The results showed that Lavandula angustifolia Mill. extract showed a certain inhibitory activity on acetylcholin esterase (inhibition rate was 3.00%~82.78%).7 Lavandula angustifolia Mill. extracts showed significant inhibition on acetylcholin esterase(inhibition rate was higher than 50%),while 4 Lavandula angustifolia Mill. extracts showed faint inhibition on acetylcholin esterase (inhibition rate was less than 10%).Half of Lavandula angustifolia Mill. extract showed the content of polyphenols was higher than 20 μg·mL-1.The most of extract showed a certain inhibitory activity on acetylcholin esterase.Through the correlation analysis,polyphenols may not be the main active substance.
参考文献/References:
［1］Aisen P S,Davis K L.The search for disease-modifying treatment for Alzheimer’s disease[J].Neurology,1997,48(5 Suppl 6):S35.
备注/Memo
收稿日期：2019-02-16