GUO Cui-ling,ZHANG Ying.Inhibiting Effect of Pomace Extract of Vaccinium uliginosum to Tyrosinase[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(12):75-78.[doi:10.11942/j.issn1002-2767.2018.12.0075]
蓝莓果渣提取物对酪氨酸酶的抑制作用
- Title:
- Inhibiting Effect of Pomace Extract of Vaccinium uliginosum to Tyrosinase
- Keywords:
- Vaccinium uliginosum pomace; arbutin; tyrosinase; inhibiting effect
- 文献标志码:
- A
- 摘要:
- 为促进蓝莓资源的综合利用,以乙醇为溶剂,超声提取,并采用紫外分光光度计对醇提液中熊果苷含量进行了测定,同时以水杨酸为阳性对照,测定了蓝莓果渣醇提液对酪氨酸酶的抑制作用。结果表明:蓝莓果渣含熊果苷0.18%,其提取液对酪氨酸酶有抑制作用,随含量增加抑制活性增加,且抑制作用与水杨酸低剂量组相当。
- Abstract:
- In order to promote the comprehensive utilization of Vaccinium uliginosum resources, ethanol was used as solvent for ultrasonic extraction, and the content of arbutin in ethanol extract was determined by ultraviolet spectrophotometer.At the same time, the inhibition of Vaccinium uliginosum pomace ethanol extract was determined by using salicylic acid as positive control.The results showed that the Vaccinium uliginosum pomace contained 0.18% arbutin, and the extract had inhibitory effect on tyrosinase.The inhibitory activity increased with the increase of the content, and the inhibitory effect was similar to that of salicylic acid low dose group.
参考文献/References:
[1]王玉洁,陈敏,张扬,等.蓝莓果渣中熊果苷提取工艺的研究[J].中国林副特产,2012(2): 23-25.[2]王姗姗,孙爱东,李淑燕.蓝莓的保健功能及其开发应用[J].中国食物与营养,2010(6): 17-20.[3]李颖畅.蓝莓花色苷提取纯化及生理功能研究[D].沈阳:沈阳农业大学,2008.[4]宋康康,邱凌,黄璜,等.熊果甙作为化妆品添加剂对酪氨酸酶抑制作用[J].厦门大学学报(自然科学版),2003(6): 791-794.[5]张丽娟.苯甲酸衍生物对酪氨酸酶的效应及其抑菌作用[D].厦门:厦门大学,2006.[6]付士慧,朱文学.梨花熊果苷提取工艺及体外抗氧化性初步研究[J].河南科技大学学报(自然科学版),2017,38(5): 55-59.[7]龚盛昭,杨卓如,程江.肉桂酸抑制酪氨酸酶催化反应的动力学研究[J].高校化学工程学报,2007(2): 345-349.[8]张春乐.抑制剂对蘑菇酪氨酸酶的抑制效应及抗菌活性[D].厦门:厦门大学,2007.[9]Chen Q X,Kubo I.Kinetics of mushroom tyrosinase inhibition by quercetin [J].Journal of Agricultural and Food Chemistry,2002,50(14): 4108-4112.
备注/Memo
收稿日期:2018-08-15