XUAN Yang,XU Hong-guo,ZHONG Juan-juan,et al.Study on Rapid Propagation Techniques of Small Fruit Watermelon[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(11):45-47.[doi:10.11942/j.issn1002-2767.2017.11.0045]
小果型西瓜快速繁殖技术研究
- Title:
- Study on Rapid Propagation Techniques of Small Fruit Watermelon
- 文章编号:
- 1002-2767(2017)11-0045-03
- Keywords:
- small fruit watermelon; tissue culture; rapid propagation
- 分类号:
- S651
- 文献标志码:
- A
- 摘要:
- 为建立小果型西瓜组织培养快速繁殖方法,对小果型西瓜重要种质材料的保存扩繁提供技术支持。以小果型西瓜为材料、MS培养基为基本培养基,通过不同种类和浓度的植物生长调节剂配比试验,筛选小果型西瓜快速繁殖与生根的最佳培养基组成。结果表明:子叶苗茎尖丛生芽诱导培养基MS+6-BA 1.0 mg?L -1 + NAA 0.1 mg?L -1效果较好,试管苗继代增殖最适培养基为MS+6-BA 0.5 mg?L -1+NAA 0.1 mg?L -1,诱导试管苗生根的最适培养基为1/2 MS+IBA 0.3 mg?L -1。
- Abstract:
- In order to establish tissue culture rapid propagation method of small fruit watermelon,provide technical support for small watermelon germplasm preservation and propagation of important materials.The small fruit watermelon was used as the material and MS medium as the basic medium,the optimum medium composition for rapid propagation and rooting of small fruit watermelon was screened through different kinds and concentrations of plant growth regulators.The results showed that MS+6-BA 1 mg? L -1+NAA 0.1 mg?L -1 was the best medium for cotyledon bud shoot induction,plantlet propagation the optimum culture medium was MS+6-BA 0.5 mg?L -1+NAA 0.1 mg?L -1,the optimum medium for rooting of plantlets in vitro was 1/2 MS+IBA 0.3 mg?L -1.
参考文献/References:
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备注/Memo
收稿日期:2017-09-13基金项目:2016年黑龙江省大学生创新创业训练计划资助项目(201610221031)第一作者简介:宣杨(1995-),男,黑龙江省大庆市人,在读学士,从事西瓜生物技术研究。E-mail:766762723@qq.com。