[1]宣 杨,徐洪国,仲娟娟,等.小果型西瓜快速繁殖技术研究[J].黑龙江农业科学,2017,(11):45-47.[doi:10.11942/j.issn1002-2767.2017.11.0045]
　XUAN Yang,XU Hong-guo,ZHONG Juan-juan,et al.Study on Rapid Propagation Techniques of Small Fruit Watermelon[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(11):45-47.[doi:10.11942/j.issn1002-2767.2017.11.0045]
点击复制

小果型西瓜快速繁殖技术研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年11期 页码: 45-47 栏目: 出版日期: 2017-11-10
Title:
Study on Rapid Propagation Techniques of Small Fruit Watermelon
文章编号:
1002-2767（2017）11-0045-03
作者:
宣 杨; 徐洪国; 仲娟娟; 王英学; 祁宏英
齐齐哈尔大学 生命科学与农林学院，黑龙江 齐齐哈尔 161006
Author(s):
XUAN Yang; XU Hong-guo; ZHONG Juan-juan; WANG Ying-xue; QI Hong-ying
XUAN Yang,XU Hong-guo,ZHONG Juan-juan,WANG Ying-xue,QI Hong-ying
关键词:
小果型西瓜; 组织培养; 快速繁殖
Keywords:
small fruit watermelon;  tissue culture;  rapid propagation
分类号:
S651
DOI:
10.11942/j.issn1002-2767.2017.11.0045
文献标志码:
A
摘要:
为建立小果型西瓜组织培养快速繁殖方法，对小果型西瓜重要种质材料的保存扩繁提供技术支持。以小果型西瓜为材料、MS培养基为基本培养基，通过不同种类和浓度的植物生长调节剂配比试验，筛选小果型西瓜快速繁殖与生根的最佳培养基组成。结果表明：子叶苗茎尖丛生芽诱导培养基MS+6-BA 1.0 mg?L -1 + NAA 0.1 mg?L -1效果较好，试管苗继代增殖最适培养基为MS+6-BA 0.5 mg?L -1+NAA 0.1 mg?L -1，诱导试管苗生根的最适培养基为1/2 MS+IBA 0.3 mg?L -1。
Abstract:
In order to establish tissue culture rapid propagation method of small fruit watermelon,provide technical support for small watermelon germplasm preservation and propagation of important materials.The small fruit watermelon was used as the material and MS medium as the basic medium,the optimum medium composition for rapid propagation and rooting of small fruit watermelon was screened through different kinds and concentrations of plant growth regulators.The results showed that MS+6-BA 1 mg? L -1+NAA 0.1 mg?L -1 was the best medium for cotyledon bud shoot induction,plantlet propagation the optimum culture medium was MS+6-BA 0.5 mg?L -1+NAA 0.1 mg?L -1,the optimum medium for rooting of plantlets in vitro was 1/2 MS+IBA 0.3 mg?L -1.

参考文献/References:

[1]肖光辉.小果型西瓜早春设施再生栽培技术[J].长江蔬菜,2011(1): 13-15.
[2]李婷,曾剑波,陈艳利,等.北京地区秋季大棚小果型西瓜吊蔓栽培技术[J].中国瓜菜,2013,26(6): 52-53.
[3]王迎儿,马凯慧,陆袁波,等.“甬砧5号”嫁接小西瓜立架栽培关键技术[J].宁波农业科技,2014(2): 24-25.
[4]Li J,Tang Y,Qin Y,et al.Agrobacterium-mediated transformation of watermelon (Citrullus lanatus)[J].African Journal of Biotechnology,2012,11(24): 6450-6456.
[5]张志忠,吴菁华,吕柳新.根癌农杆菌介导的西瓜遗传转化研究[J].果树学报,2005,22(2): 134-137.
[6]李娟,李万宁,唐懿,等.根癌农杆菌介导的西瓜遗传转化研究进展[J].中国蔬菜,2010(8): 7-13.
[7]任俊杰,王丽霞,高洪波,等.农杆菌介导的西瓜枯萎病菌遗传转化[J].植物保护,2015,41(1):93-97.
[8]杨国志，张明方，顾学根，等.NaN3处理条件下西瓜直接再生试验体系研究［J］.长江蔬菜，2009（6）：11-14.
[9]王建书,孙松松,刘浩,等.西葫芦茎尖离体培养和快速繁殖技术[J].北方园艺,2015(22): 96-98.
[10]昌正兴,马陆平,周泉,等.无籽西瓜离体快繁技术研究[J].湖南农业科学,2008(1):15-16.
[11]孙光英.无籽西瓜离体培养及再生植株的快速繁殖技术分析[J].耕作与栽培,2004(4): 39-39.

相似文献/References:

[1]孙岩,张宏纪,王广金,等.航天处理对小麦组织培养效果的研究[J].黑龙江农业科学,2014,(11):1.
　SUN Yan,ZHANG Hong-ji,WANG Guang-jin,et al.Study on the Effect of Space Treatment on Wheat Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(11):1.
[2]王碧才,丰 锋.文心兰不定芽生根培养基配方的优化与移栽基质筛选[J].黑龙江农业科学,2014,(11):12.
　WANG Bi-cai,FENG Feng.Optimization of Rooting Medium and Screening of Transplanting Medium of Oncidium[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(11):12.
[3]曹天旭,宿肇辉,韩超慧.铁皮石斛组培快繁技术的研究[J].黑龙江农业科学,2014,(05):30.
　CAO Tian-xu,SU Zhao-hui,HAN Chao-hui.Study on Tissue Culture and Rapid Propagation of Dendrobium candidum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(11):30.
[4]唐文杰,王玉,周泉澄,等.马齿苋组培与耐盐筛选[J].黑龙江农业科学,2013,(03):7.
　TANG Wen-jie,WANG Yu,ZHOU Quan-cheng,et al.Tissue Culture and Salt Resistance Selection of Portulaca oleracea L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(11):7.
[5]尹相博,于咏梅,于立芝.红树莓组织培养研究进展[J].黑龙江农业科学,2013,(02):140.
　YIN Xiang-bo,YU Yong-mei,YU Li-zhi.Review of Progress in Tissue Culture of Red Raspberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(11):140.
[6]陈荣珠,沈应柏,罗晓芳,等.连香树组织培养及植株再生体系的初步研究[J].黑龙江农业科学,2012,(01):16.
　CHEN Rong-zhu,SHEN Ying-bai,LUO Xiao-fang,et al.Research on Tissue Culture and Plant Regeneration of Cercidiphyllum japonicum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(11):16.
[7]曹羽.香花槐组织培养研究现状[J].黑龙江农业科学,2012,(04):142.
　CAO Yu.Research Advance in Tissue Culture of Robinia pseudoacaia ‘Idaho’[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(11):142.
[8]张瑜,柏彦伸,孙靖靓,等.活血丹子叶无性系建立的研究[J].黑龙江农业科学,2012,(11):21.
　ZHANG Yu,BAI Yan-shen,SUN Jing-liang,et al.Study on Establishment of Clone for Giechoma longituba[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(11):21.
[9]时群,何贵整,陈乃明,等.木薯产业化组培育苗技术研究[J].黑龙江农业科学,2012,(11):25.
　SHI Qun,HE Gui-zheng,CHEN Nai-ming,et al.Study on Industrialization Tissue CultureSeedlings Technology of Cassava[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(11):25.
[10]张元国,李 芳,包艳存,等.芦笋全雄品种WF-8茎尖组培快繁技术研究[J].黑龙江农业科学,2012,(12):21.
　ZHANG Yuan-guo,LI Fang,BAO Yan-cun,et al.Study on Tissus Culture Rapid Reproduction Technology of Stem Tip of All Male Asparagus Cultivar WF-8[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(11):21.

备注/Memo

收稿日期：2017-09-13基金项目：2016年黑龙江省大学生创新创业训练计划资助项目（201610221031）第一作者简介：宣杨（1995-），男，黑龙江省大庆市人，在读学士，从事西瓜生物技术研究。E-mail：766762723@qq.com。

更新日期/Last Update: 2018-02-22