[1]胡秉芬,黄华梨,季元祖,等.不同溶剂浸提枣树叶片叶绿素效果的研究[J].黑龙江农业科学,2017,(11):48-54.[doi:10.11942/j.issn1002-2767.2017.11.0048]
　HU Bing-fen,HUANG Hua-li,JI Yuan-zu,et al.Effects of Different Solutions on Chlorophyll Extraction from Jujube Leaves[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(11):48-54.[doi:10.11942/j.issn1002-2767.2017.11.0048]
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不同溶剂浸提枣树叶片叶绿素效果的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年11期 页码: 48-54 栏目: 出版日期: 2017-11-10
Title:
Effects of Different Solutions on Chlorophyll Extraction from Jujube Leaves
文章编号:
1002-2767（2017）11-0048-07
作者:
胡秉芬; 黄华梨; 季元祖; 赵晓芳; 戚建莉; 张露荷; 张广忠
甘肃省林业科学研究院，甘肃 兰州 730020
Author(s):
HU Bing-fen; HUANG Hua-li; JI Yuan-zu; ZHAO Xiao-fang; QI Jian-li; ZHANG Lu-he; ZHANG Guang-zhong
Gansu Research Academy of Forestry Science and Technilogy,Lanzhou,Gansu 730020
关键词:
枣树; 叶绿素; 浸提; 有机溶剂
Keywords:
jujube trees chlorophyll;  extraction;  organic solvent
分类号:
S665.1
DOI:
10.11942/j.issn1002-2767.2017.11.0048
文献标志码:
A
摘要:
为探讨枣树叶片叶绿素的最佳提取方法,以16种枣树品种为材料，用95%乙醇、乙醇丙酮混合液（1∶1）和80%丙酮分别浸提不同枣树品种叶片叶绿素，用分光光度法测定叶绿素含量，对不同浸提液的叶绿素含量进行了分析。结果表明：80%丙酮浸提枣树叶片叶绿素的效果不佳；95%乙醇和乙醇丙酮混合液（1∶1）浸提枣树叶片叶绿素的效果好，且水平相当。但丙酮对人体的健康危害较大，浸提枣树叶片叶绿素应首选95%乙醇。
Abstract:
In order to study the optimal extraction method effect of chlorophyll from jujube leaves,chlorophyll were extracted from 16 kinds of jujube varieties leaves using 95% ethanol,mixture of ethanol and acetone (1∶1) and 80% acetone respectively,chlorophyll content was determined by spectrophotometry,the content of chlorophyll in different extracts was studied.The results showed that 80% acetone was not ideal for the extraction of chlorophyll from the leaves of jujube trees; 95% ethanol and mixed liquid of ethanol and acetone (1∶1) have a good effect on the extraction of chlorophyll from the leaves of jujube trees,and the extraction level was quite the same.But acetone was harmful to human health.95% ethanol was the first choice to extract chlorophyll from leaves of Chinese jujube.

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备注/Memo

收稿日期：2017-09-20基金项目：林业公益性行业科研专项资助项目（201304702）第一作者简介：胡秉芬（1969-），女，甘肃省景泰县人，博士，副研究员，从事经济林育种研究。E-mail：bf9134hu@163.com。通讯作者：黄华梨（1963-），男，四川省广元市人，学士，正高级工程师，从事经济林和自然保护管理与研究。E-mail：gshhl@hotmail.com。

更新日期/Last Update: 2018-02-22