[1]鲁珊,毛彩云,肖荷霞.应用叶绿素荧光法鉴定氮素对诸葛菜组培苗光合能力的影响[J].黑龙江农业科学,2017,(10):37-40.[doi:10.11942/j.issn1002-2767.2017.10.0037]
　LU Shan,MAO Cai-yun,XIAO He-xia.Effects of Nitrogen on Photosynthetic Capacity of Orychophragmcs violaceus Tissue Culture Seedling by Chlorophyll Fluorescence Method[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(10):37-40.[doi:10.11942/j.issn1002-2767.2017.10.0037]
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应用叶绿素荧光法鉴定氮素对诸葛菜组培苗光合能力的影响

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年10期 页码: 37-40 栏目: 出版日期: 2017-10-10
Title:
Effects of Nitrogen on Photosynthetic Capacity of Orychophragmcs violaceus Tissue Culture Seedling by Chlorophyll Fluorescence Method
文章编号:
1002-2767（2017）10-0037-04
作者:
鲁珊; 毛彩云; 肖荷霞
沧州市农林科学院，河北 沧州 061001
Author(s):
LU Shan; MAO Cai-yun; XIAO He-xia
Cangzhou Academy of Agriculture and Forestry Sciences，Cangzhou，Hebei 061001
关键词:
：组培苗; 调制叶绿素荧光仪; 光合能力; 氮素
Keywords:
tissue culture seedling; pulse-amplitude-modulation; photosynthetic capacity; nitrogen
分类号:
S644.9
DOI:
10.11942/j.issn1002-2767.2017.10.0037
文献标志码:
A
摘要:
为改良培养基的配方，利用调制叶绿素荧光仪，对3个氮素水平（低氮0.356 g?L-1、中氮0.632 g?L-1、高氮1.159 g?L-1、对照组0.908 g?L-1）组织培养条件下诸葛菜组培苗的光合特性进行了研究。结果表明：3个氮素水平下诸葛菜组培苗光合能力大小顺序为中氮>对照组>高氮>低氮，与氮素并不呈正比关系，在一定浓度范围随氮素的增加而增加，增加到一定程度又出现降低趋势，因此配置适量氮素才可以有效利用且不浪费资源。
Abstract:
PAM chlorophyll fluorometer was used to measure fluorescence parameters of ?Orychophragmus violaceus?plantlets in vitro at three different nitrogen levels,and the fluorescence parameters was compared.The results showed that the photosynthetic capacity of Orychophragmus violaceus plantlets in vitro was the strongest under middle nitrogen level,the photosynthetic capacity under high nitrogen level was weaker than control group and the photosynthetic capacity under low nitrogen level was the weakest.It was not a positive correlation between photosynthetic capacity and nitrogen rates,therefore,the appropriate amount of nitrogen in the medium could be effectively used and not waste of resources.

参考文献/References:

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备注/Memo

收稿日期：2017-07-18

第一作者简介：鲁珊（1984-），女，河北省沧州市人，硕士，助理研究员，从事玉米育种研究。E-mail：lushan_607@126.com。?
通讯作者：肖荷霞（1963-），女，河北省沧州市人，硕士，研究员，从事玉米栽培育种研究。E-mail：461159070@qq.com。

更新日期/Last Update: 2018-01-31