[1]吕德任,黄赛,戚华沙,等.火炬姜种子无菌快速繁殖技术研究[J].黑龙江农业科学,2016,(09):19-24.[doi:10.11942/j.issn1002-2767.2016.09.0019]
　LYU De-ren,HUANG Sai,QI Hua-sha,et al.Study on Seeds Sterile Rapid Propagation of Phaeomeria magnifica(Roscoe) K.Shum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(09):19-24.[doi:10.11942/j.issn1002-2767.2016.09.0019]
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火炬姜种子无菌快速繁殖技术研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2016年09 页码: 19-24 栏目: 出版日期: 2016-09-10
Title:
Study on Seeds Sterile Rapid Propagation of Phaeomeria magnifica(Roscoe) K.Shum
文章编号:
1002-2767（2016）09-0019-05
作者:
吕德任; 黄赛; 戚华沙; 王景飞; 符瑞侃; 潘梅
（海南省农业科学院 热带园艺研究所，海南 海口 571100）
Author(s):
LYU De-ren; HUANG Sai; QI Hua-sha; WANG Jing-fei; FU Rui-kan; PAN Mei
(Tropical Horticuture Research Institute of Hainan Academy of Agricultural Sciences,Haikou,Hainan 571100)
关键词:
火炬姜; 种子; 丛生芽; 组织培养; 快速繁殖
Keywords:
Phaeomeria magnifica;  seed;  fasciculate buds;  tissue culture;  rapid propagation
分类号:
S685.15
DOI:
10.11942/j.issn1002-2767.2016.09.0019
文献标志码:
A
摘要:
为了加速火炬姜无菌培养，以火炬姜成熟种子作为外植体，通过比较MS无机盐浓度和植物生长调节剂种类及浓度配比、以及生根苗的移栽基质,建立火炬姜的组培快繁技术。结果表明：种子在培养基MS+6-BA 2.0 mg?L-1中萌发；培养基MS+6-BA 3.0 mg?L-1 +NAA 0.5 mg?L-1有利于丛生芽的生长发育，30 d增殖系数为5.75；3/4MS+ NAA 1.0 mg?L-1适宜诱导生根获得再生植株，生根率100%；生根苗移栽于椰糠中成活率98%，运用该组培快繁技术，可以高效繁殖火炬姜种苗。
Abstract:
In order to accelerate the sterile culture of Phaeomeria magnifica,using the seeds as explants,the techniques of tissue culture and rapid propagation were studied by comparing with different MS inorganic salt concentrations,plant growth regulator types,concentration ratio and transplanting substrates.The results showed that seeds sprouted buds in the medium MS+6-BA 2.0 mg?L-1; MS+6-BA 3.0 mg?L-1 +NAA 0.5 mg?L-1 could effectively induce fasciculate buds,the propagation coefficient was 5.75 every 30 days; the most optimum rooting medium was 3/4MS+ NAA 1.0 mg?L-1,and the rooting rate was 100%.The rooting seedlings were effectively transplanted in coconut chaff,the survival rate was 98%.Using the technology of tissue culture and rapid propagation of Phaeomeria magnifica could efficiently breeded seedlings.

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备注/Memo

收稿日期：2016-07-20

基金项目：〖JP4〗海南省科研院所技术开发研究专项资助项目（KYYS-2015-14）
第一作者简介：吕德任（1980-），男，海南省澄迈县人，助理园艺师，从事植物组织培养研究。E-mail：644615466@qq.com。
通讯作者：潘梅（1962-），女，广西壮族自治区隆安县人，农学学士，高级园艺师，从事植物组织培养研究与开发利用工作。E-mail：panmei200@sina.com。

更新日期/Last Update: 2016-12-14