ZHANG Xue-mei,FAN Zeng-li.Effect of Simulated Warming on Physiology Resistance of Epimedium wushanense[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(07):80-85.[doi:10.11942/j.issn1002-2767.2016.07.0080]
不同增温处理对巫山淫羊藿抗性生理的影响
- Title:
- Effect of Simulated Warming on Physiology Resistance of Epimedium wushanense
- 文章编号:
- 1002-2767（2016）07-0080-06
- 分类号:
- S567.239；Q945
- 文献标志码:
- A
- 摘要:
- 为探究淫羊藿栽培技术，促进其生长发育，满足市场需求，以巫山淫羊藿为研究对象，通过开顶式气室（OTC，open-top chambers）对移栽的巫山淫羊藿进行不同程度的温度处理，探讨增温对巫山淫羊藿抗性生理特性的影响，揭示其对温度的响应情况。结果表明：在增温条件下，巫山淫羊藿不同器官表现出不同的抗性能力。随着温度升高，所有器官中的MDA和H2O2含量基本上表现为降低趋势。增温对根、茎中POD活性影响不显著；但对叶和花的影响显著。增温对SOD活性影响显著，但对CAT活性影响不显著。增温对VE和VC影响显著。增温同样能显著影响羟自由基和总抗氧化能力及蛋白质含量。+2 ℃增温的条件下有利于巫山淫羊藿的生长，而超过这个限度会对植物起到抑制的作用。
- Abstract:
- In order to study the cultivation techniques of Epimedium wushanense,promote the growth and satisfy the market demand,taking Epimedium wushanense as materials,an open top chamber (OTC) warming system was established to study the impact of simulated warming on physiology resistance of Epimedium wushanense.The results showed that different organs of Epimedium wushanense showed different resistant ability in warming condition.The content of MDA and H2O2 in all organs were significantly decreased with the rise in temperature. The effect of warming on the POD activity of root and stem was not significant except the leaf and flower. The effect of warming on the SOD activity, the content of VC and VE, hydroxy free radical ability and T-AOC was significant in all organs,but on the CAT was not significant.In conclusion the condition of +2 ℃ warmingis conducived to the growth of Epimedium wushanense.
参考文献/References:
[1]Metz B,Davidson O R,Bosch P R,et al.Climate Change in 2007: Mitigation
［
M
］
.Cambridge University Press,Cambridge,UK.
，
2007.
[2]Foyer C H,Vanacker H,Goméz L D,et al.Regulation of photosynthesis and antioxidant metabolism in maize leaves at optimal and chilling temperatures: review[J].Plant Physiol.Biochem.2002，40: 659-668. [3]Schonhof I,Klring H P,Krumbein A,et al.Schreiner M,Effect of temperature increase under low radiation conditions on phytochemicals and ascorbic acid in greenhouse grown broccoli[J].Agr.Ecosyst.Environ,2007,119: 103-111. [4]Tegelberg R,Julkunen-Tiitto R,Vartiainen M,et al.Exposures to elevated CO2,elevated temperature and enhanced UV-B radiation modify activities of polyphenol oxidase and guaiacol peroxidase and concentrations of chlorophylls,polyamines and soluble proteins in the leaves of Betulapendula seedlings[J].Environ.Exp.Bot,2008,62: 308-315. [5]Xin Z,Browse J.Cold comfort farm: the acclimation of plants to freezing temperatures［J］.Plant Cell and Environment,2000,23:893-902. [6]Nakano Y,Asada K.Purification of ascorbate peroxidase in spinach chloroplasts，its inactivation in ascorbate depleted medium and reactivation by monodehydroascorbateradicalV［J］.Plant Cell Physiology,1987,28: 131-140. [7]高泽梅,权秋梅,肖娟,等.金城山柔毛淫羊藿的开花物候与生殖特性[J].广西植物,2014(1): 009. [8]权秋梅,高泽梅,黎云祥.不同生境中柔毛淫羊藿形态特征及其有效成分差异分析[J].广西植物,2012,32(33): 350-354. [9]刘俊，吕波，徐朗来.植物叶片中过氧化氢含量测定方法的改进[J].生物化学与生物物理进展，2000，27（5）：548-551. [10]李忠光,李江鸿,杜朝昆,等.在单一提取系统中同时测定五种植物抗氧化酶[J].云南师范大学学报:自然科学版,2002,22(6): 44-48. [11]郑京平.水果、蔬菜中维生素C含量的测定——紫外分光光度快速测定方法探讨[J].光谱实验室,2006,23(4): 731-735. [12]李英丽,邓连琴,果秀敏,等.同步荧光法测定蔬菜中维生素 E 含量[J].河北大学学报：自然科学版，2009，29（4）：412-415. [13]滕中华,智丽,宗学凤,等.高温胁迫对水稻灌浆结实期叶绿素荧光、抗活性氧活力和稻米品质的影响[J].作物学报,2008,34(9): 1662-1666. [14]王孝平，邢树礼.考马斯亮蓝法测定蛋白含量的研究[J].天津化工，2009，23（3）：40-41. [15]杨淑慎,高俊凤.活性氧.自由基与植物的衰老[J].西北植物学报,2001,21(2): 215-220. [16]Jones D L,Kochian L V.Aluminum interaction with plasma membrane lipids and enzyme metal binding sites and its potential role in cytotoxicity [J].Febs letters,1997,400(1): 51-57. [17]江福英,李延,翁伯琦.植物低温胁迫及其抗性生理[J].福建农业学报,2002,17(3): 190-195. [18]肖凯，张荣铣，钱维朴.氮素营养调控小麦旗叶衰老和光合功能衰退的生理机制[J].植物营养与肥料学报,1998(4)：371-378. [19]Schonhof I,Klring H P,Krumbein A,et al.Schreiner M,Effect of temperature increase under low radiation conditions on phytochemicals and ascorbic acid in greenhouse grown broccoli[J].Agr.Ecosyst.Environ,2007,119: 103-111. [20]ncel I,Keles Y,stün A S.Interactive effects of temperature and heavy metal stress on the growth and some biochemical compounds in wheat seedlings[J].Environ.Pollut,2000,107: 315-320. [21]张磊,吴冬云,朱碧岩,等.灌浆期不同温光对水稻叶、籽粒可溶性蛋白质及可溶性糖动态变化的影响[J].华南师范大学学报：自然科学版,2002（2）:98-101. [22]武敬亮,苏智先,田桂香,等.淫羊藿研究新进展[J].中医药学报,2004,39(3):69-72,84. [23]王小丽,裴玉贺,张恩盈,等.低温胁迫下玉米幼苗的几种生理生化指标的变化[J].植物生理学通讯,2009,49(5):487-490. [24]李文峰,孟亚利,陈兵林,等.气象因子对棉籽脂肪和蛋白质含量的影响[J].生态学报,2009（4）: 1832-1839.
备注/Memo
收稿日期：2016-06-07