[1]赵玉芬,储博彦,刘满光,等.卡拉胶对大花萱草试管苗生长的影响[J].黑龙江农业科学,2015,(10):24-26.[doi:10.11942/j.issn1002-2767.2015.10.0024]
　ZHAO Yu-fen,CHU Bo-yan,LIU Man-guang,et al.Effect of Carrageenan on the Tube Seedings of Hemerocallis hybridas [J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(10):24-26.[doi:10.11942/j.issn1002-2767.2015.10.0024]
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卡拉胶对大花萱草试管苗生长的影响

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2015年10 页码: 24-26 栏目: 出版日期: 2015-10-10
Title:
Effect of Carrageenan on the Tube Seedings of Hemerocallis hybridas
文章编号:
1002-2767（2015）10-0024-03
作者:
赵玉芬; 储博彦; 刘满光; 尹新彦; 李金霞
河北省林业科学研究院/河北省林木良种工程技术中心，河北 石家庄 050061
Author(s):
ZHAO Yu-fen 1; 2; CHU Bo-yan 1; 2; LIU Man-guang 1; 2; YIN Xin-yan 1; 2; LI Jin-xia 1; 2
1.Hebei Academy of Forestry Science,Shijiazhuang，Hebei 050061; 2.Hebei Engineering Research Center for Trees Varieties，Shijiazhuang，Hebei 050061
关键词:
卡拉胶; 大花萱草; 试管苗; 增殖系数; 生根率
Keywords:
carrageenan; Hemerocallis hybridas;  tube seeding;  differentiation coefficient;  rooting rate
分类号:
S688
DOI:
10.11942/j.issn1002-2767.2015.10.0024
文献标志码:
A
摘要:
为选择理想的琼脂及琼脂的替代物，以降低大花萱草组培苗的生产成本，收集了3种市售琼脂和1种卡拉胶，比较不同浓度的卡拉胶和琼脂对大花萱草试管苗生长的影响，降低大花萱草组培苗的生产成本。结果表明：卡拉胶对试管苗的分化生长影响表现在其分化增殖系数均高于市售的3种琼脂。卡拉胶4 g?L-1为试管苗分化增殖的最佳固化剂及浓度。在试管苗生根方面，筛选出了市售琼脂3号4 g?L-1 为最佳生根固化剂及浓度。因此，大花萱草试管苗分化阶段固化剂采用卡拉胶4 g?L-1，生根阶段固化剂采用琼脂3号4 g?L-1，达到了降低生产成本的目的。
Abstract:
In order to choose the ideal AGAR and AGAR substitute and to reduce the production cost of Hemerocallis hybridas tissue culture seedling,the effects among three types of AGAR and a carrageenan on the tube seedings of Hemerocallis hybridas were studied.The results showed that the differentiation coefficient of tube seedlings in the carrageenan medium was bigger than them in the agar mediums.Considering solidification effect,4 g?L-1 carrageenan was the best solidifying agent.The suitable medium for rooting had also selected,it was 4 g?L-1 of No.3 AGAR.The rooting rate and the number of the seeding root were both bigger than the other.On the tissue culture of the Hemerocallis hybridas,the solidifying agent might use 4 g?L-1 carrageenan during differentiation phase,and use 4 g?L-1 of No.3 AGAR during rooting phase,and production cost reduced.

参考文献/References:

［1］徐强,管华诗.卡拉胶研究的发展及现状[J].青岛海洋大学学报,1995(增刊):117-118.
[2]陈卫东.卡拉胶的性能及某些新应用[J].广东化工,1999(2):12-16.
[3]华汉峰.一种用途广泛的海藻胶一卡拉胶[J].水产科学,1990,9(1):37-40.
[4]翟红莲,纪守兴,赵学燕，等.植物组织培养培养基凝固剂替代物的研究[J].山东林业科技,2009(2):5-9.
[5]张志东,贺红霞,刘立波，等.卡拉胶对非洲菊组织培养苗影响研究[C].中国青年农业科学学术年报,2002：256-260.
[6]刘忠玲.不同凝固剂在马铃薯试管苗培养中的对比试验[J].陕西农业科学,2002(12):11-12.

备注/Memo

收稿日期：2015-04-09
基金项目：河北省科技厅科技支撑资助项目(11220608D、15226406D)
第一作者简介：赵玉芬（1974-），女，河北省阜城县人，学士，高级工程师，从事园林植物的栽培及组织培养技术研究。E-mail：hbzyf74@163.com。

更新日期/Last Update: 2016-03-29