[1]温秀萍,林青青,杨成梓,等.闽产石仙桃无菌播种与组培快繁技术研究初报[J].黑龙江农业科学,2015,(08):19-21.[doi:10.11942/j.issn1002-2767.2015.08.0019]
　WEN Xiu ping,LIN Qing qing,YANG Cheng zi,et al.Preliminary Report on Aseptic Seeding and Tissue Culture of Pholidota chinensis Lindl.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(08):19-21.[doi:10.11942/j.issn1002-2767.2015.08.0019]
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闽产石仙桃无菌播种与组培快繁技术研究初报

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2015年08 页码: 19-21 栏目: 出版日期: 2015-08-10
Title:
Preliminary Report on Aseptic Seeding and Tissue Culture of Pholidota chinensis Lindl.
文章编号:
1002-2767（2015）08-001903
作者:
温秀萍; 林青青; 杨成梓; 于虹敏
福建中医药大学 药学院，福建 福州 350122
Author(s):
WEN Xiuping; LIN Qingqing; YANG Chengzi; YU Hongmin
College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122
关键词:
石仙桃; 种子; 无菌播种; 快繁技术
Keywords:
Pholidota chinensis;  seed;  aseptic seeding;  rapid propagation
分类号:
R282.2
DOI:
10.11942/j.issn1002-2767.2015.08.0019
文献标志码:
A
摘要:
为构建石仙桃无菌播种与组培快繁技术，对石仙桃的无菌播种与快繁技术进行了相关研究。结果表明：石仙桃种子最佳消毒溶液为0.1%升汞，处理时间为8 min；种子萌发最佳基本培养基为N6培养基，N6+1.0 mg·L-1 6BA+1.0 mg·L-1 NAA激素组合的培养基对种子萌发也同样合适；不同植物激素组合对石仙桃幼苗生长影响无显著性差异，N6+1.5 mg·L-1 6BA+0.5 mg·L-1 NAA 效果较好；在N6+0.5 mg·L-1 6BA+1.5 mg·L-1 NAA+75 g·L-1香蕉培养基上生根率达85%，通过植物组织培养技术，成功构建了石仙桃种子的非共生萌发和快繁技术体系。
Abstract:
In order to build the seed rapid propagation technology of Pholidota chinensis,the aseptic seeding and tissue culture of Pholidota chinensis were studied.The results showed that the disinfection treatment with 0.1% HgCl2 solution for 8 min was most suitable for aseptic seeding.The best medium for seed germination was N6 medium,the optimum medium of N6+6BA 1.0 mg·L-1+NAA 1.0 mg·L-1 was also suitable for seed germination; Different combinations of 6BA and NAA didn’t show significant difference on the growth of young seedlings of Pholidota chinensis,and the appropriate medium was N6+6BA 1.5 mg·L-1+NAA 0.5 mg·L-1.Seedling’s rooting rate was 85% on the medium of N6+6BA 0.5 mg·L-1+NAA 1.5 mg·L-1+75 g·L-1 banana.The system of asymbiotic seed germination and rapid propagation of Pholidota chinensis was constructed successfully by the technology of plant tissue culture.

参考文献/References:

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备注/Memo

福建省教育厅A类科技资助项目（JA14165）；福建中医药大学校管课题资助项目（X2013013）温秀萍（1984-），女，福建省福州市人，硕士，研究实习员，从事药用植物资源与种质选育研究。E-mail：375706654@qq.com。

更新日期/Last Update: 2016-09-09