[1]曹芳凤,王冠,张宵娟,等.牛耳秋海棠的组培快繁研究[J].黑龙江农业科学,2015,(08):14-18.[doi:10.11942/j.issn1002-2767.2015.08.0014]
　CAO Fang feng,WANG Guan,ZHANG Xiao juan,et al.Research on the Tissue Culture About Begonia sanguinea Raddi[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(08):14-18.[doi:10.11942/j.issn1002-2767.2015.08.0014]
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牛耳秋海棠的组培快繁研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2015年08 页码: 14-18 栏目: 出版日期: 2015-08-10
Title:
Research on the Tissue Culture About Begonia sanguinea Raddi
文章编号:
1002-2767（2015）08-0014-05
作者:
曹芳凤1; 王冠2; 张宵娟3; 连芳青3
1.吉安职业技术学院，江西 吉安 343000； 2.江西省林业调查规划设计研究院，江西 南昌 330046； 3.江西农业大学 园林与艺术学院，江西 南昌 330045
Author(s):
CAO Fangfeng1; WANG Guan2; ZHANG Xiaojuan3; LIAN Fangqing3
1.Ji’an Vocational and Technical College，Ji’an,Jiangxi 343000；2.Academy of Forest Inventory and Planning，Nanchang，Jiangxi 3300453.College of Landscape and Art，Jiangxi Agricultural University，Nanchang，Jiangxi 330045
关键词:
牛耳秋海棠; 不定芽; 愈伤组织; 组培快繁
Keywords:
Begonia sanguinea Raddi;  adventitious bud;  callus;  tissue culture
分类号:
S682
DOI:
10.11942/j.issn1002-2767.2015.08.0014
文献标志码:
A
摘要:
为促进牛耳秋海棠的快繁及工厂化生产，以牛耳秋海棠的叶片为外植体，对其离体再生培养技术进行了研究。结果表明：牛耳秋海棠最佳消毒方法为75%酒精消毒8 s，0.1%的HgCl2消毒6~7 min（滴加一滴吐温）；牛耳秋海棠愈伤组织诱导和分化的最佳培养基为MS+6BA0.5~1.0 mg·L-1+NAA0.1 mg·L-1，诱导率达85.42%~87.08%；牛耳秋海棠不定芽增殖的最适宜培养基是MS+6BA1.0 mg·L-1+NAA0.1 mg·L-1，增殖倍数高达42.43。
Abstract:
In order to improve the rapid propagation and factory production of Begonia sanguinea Raddi,taking leaves of Begonia sanguinea Raddi as explants,the tissue culture of Begonia sanguinea Raddi was studied.The results showed that the optimum sterilization time of explants were 8 s by 75% alcohol and 6~7 min by 0.1% mercuric chloride with a drop of Tween;The optimized medium for callus induction and differentiation was MS+6BA 0.5~1.0 mg·L-1+NAA 0.1 mg·L-1,the induction rate was 85.42%~87.08%; The optimal multiplication medium was MS+6BA 1.0 mg·L-1+NAA 0.1 mg·L-1,the multiplication coefficient was as high as 42.43.

参考文献/References:

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备注/Memo

曹芳凤（1989-），女，江西省南昌市人，硕士，从事园林植物栽培与繁育研究。E-mail:caoff1989@126.com。

更新日期/Last Update: 2016-09-09