[1]贾学方,王超.绿豆耐盐愈伤组织的诱导[J].黑龙江农业科学,2015,(08):10-13.[doi:10.11942/j.issn1002-2767.2015.08.0010]
　JIA Xue fang,WANG Chao.Induction of Salttolerant Callus in Mung Bean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(08):10-13.[doi:10.11942/j.issn1002-2767.2015.08.0010]
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绿豆耐盐愈伤组织的诱导

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2015年08 页码: 10-13 栏目: 出版日期: 2015-08-10
Title:
Induction of Salttolerant Callus in Mung Bean
文章编号:
1002-2767（2015）08-0010-04
作者:
贾学方; 王超
东北农业大学 园艺学院，黑龙江 哈尔滨 150030
Author(s):
JIA Xuefang; WANG Chao
College of Horticulture，Northeast Agricultural University，Harbin，Heilongjiang 150030
关键词:
绿豆; 离体培养; 愈伤组织; 耐盐性
Keywords:
mung bean;  in vitro culture; callus;  salt resistance
分类号:
S522
DOI:
10.11942/j.issn1002-2767.2015.08.0010
文献标志码:
A
摘要:
为探讨绿豆品种的耐盐性，利用离体培养技术，将吉绿3号绿豆种子在MS培养基中培养获无菌苗，然后将其子叶，下胚轴，胚根分别做为外植体放入诱导培养基产生胚性愈伤组织，培养基中放入的不同浓度NaCl。对愈伤组织盐胁迫处理，结果表明：当NaCl浓度由0．2％增加到1.0％时,愈伤组织的体积减小，颜色加深，由绿色变至黑褐色至死亡。在含有1.0％NaCl诱导培养基上的愈伤组织逐渐变成褐色，最终枯死；将经过0.6％NaCl诱导培养存活的愈伤组织、子叶、下胚轴、胚根分别转入含有0.6％、0.8%、1.0%NaCl的选择培养基（MS+6BA 0.2 mg·L-1+NAA 0.6 mg·L-1）上，进行比较，鉴定愈伤组织的耐盐性。结果显示：胚根、下胚轴、子叶愈伤组织的最佳盐胁迫浓度分别为0.4%、0.8%、0.6%。因此，下胚轴的耐盐性最好，子叶一般，胚根最差。
Abstract:
In order to research the salt resistance of mung bean varieties,through the tissue culture technology， the mung bean seed was cultured in the MS medium to get the sterile seedlings,and then serve the cotyledons,hypocotyl,embryo root as the explant into dedifferent inductive medium (according to add nutrients divided into eight kinds)and produced embryonic callus,and making the callus suffered from different salt stress processing that medium added the NaCl with different concentration.The results showed that when NaCl concentration increased from 0.2% to 1.0%,callus volume decreases and color burn became to yellowish brown and black to death.Contained 1.0% NaCl induction medium， the group of gradually turned into brown,eventually die; the survival callus,cotyledon,hypocotyl,and radicle in the 0.6% NaCl induction were transferred to contain 0.6%,0.8% and 1.0% NaCl choose medium (MS+6BA 0.2 mg·L-1+NAA 0.6 mg·L-1),salt resistance of callus was compared and identified .The results showed that the best salt stress concentration for radicle,hypocotyl and cotyledon callus were 0.4%,0.8% and 0.6% respectively.Therefore,salt resistance of hypocotyl was better than cotyledon,radicle was the worst.

参考文献/References:

［1］李敏.绿豆化学成分及药理作用的研究概况[J].上海中医药杂志,2001,20（5）：15-18. [2]张继中,何太.绿豆高产栽培技术[J].内蒙古农业科技,2011,22(1)：138-135. [3]邵红雨等NaCl对吸胀后绿豆种子发芽和幼苗生长的影响[J] .安徽农业科学，2007，19(06)：57-66. [4]许祥明,叶和春,李国凤.植物抗盐机理的研究进展[J].应用与环境生物学报,2000,35(4)：101-104. [5]范华美.NaCl胁迫下SA浸种绿豆幼苗的生长及生理特征[J].2003,33(18):125-130. [6]Petruse L M,Winicol L.Proline status in salt tolerant and salt sensitive alfalfa cell lines and plants in response to NaCl[J].Plant Physiology and Biochemistry,1997，31（6）：206-216. [7]张继中,何太.绿豆高产栽培技术[J].内蒙古农业科技,2011，29(1):123-125. [8]王丽侠.绿豆几个表型性状的遗传特性分析[J].作物学报，2008,23(6)30-35. [9]肖媛.盐渍化对绿豆品种幼苗生长耐盐性的影响[J].安徽农业科学，2006，35(21)：134-140. [10]葛春辉，计巧灵，王雪华.绿豆耐盐性愈伤组织的生理生化特性[J].植物研究，2008，36（5）：28-89.

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备注/Memo

贾学方（1989-），女，吉林省蛟河市人，在读硕士，从事蔬菜作物育种研究。E-mail：kuailefang100@126.com。王超（1966-），男，黑龙江省哈尔滨市人，教授，博导，从事蔬菜育种方面的研究。E-mail：wangchao504@126.com。

更新日期/Last Update: 2016-09-09