[1]高立宏,谢银艳,陈建国,等.开放无糖环境下不同激素对常青藤生根的影响[J].黑龙江农业科学,2015,(05):16-19.[doi:10.11942/j.issn1002-2767.2015.05.0016]
GAO Li hong,XIE Yin yan,CHEN Jian guo,et al.Effect of Different Hormones on Root Growth of Hedera nepalensis var.sinensis Under Sugar free and Open System[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(05):16-19.[doi:10.11942/j.issn1002-2767.2015.05.0016]
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GAO Li hong,XIE Yin yan,CHEN Jian guo,et al.Effect of Different Hormones on Root Growth of Hedera nepalensis var.sinensis Under Sugar free and Open System[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(05):16-19.[doi:10.11942/j.issn1002-2767.2015.05.0016]
开放无糖环境下不同激素对常青藤生根的影响
《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S]
卷:
期数:
2015年05
页码:
16-19
栏目:
出版日期:
2015-05-11
- Title:
- Effect of Different Hormones on Root Growth of Hedera nepalensis var.sinensis Under Sugar free and Open System
- 文章编号:
- 1002-2767(2015)05-0016-04
- Keywords:
- Hedera nepalensis var.sinensis; sugar free culture; NAA; IBA; 6-BA; leaf area
- 分类号:
- S687.3
- 文献标志码:
- A
- 摘要:
- 为探索开放条件下常青藤快繁的最适培养基,以应用于花卉苗木的快繁生产,以常青藤单叶节茎段为外植体,以1/2MS无糖蛭石培养基为基本培养基,研究不同浓度和组合激素对常青藤生根的影响。结果表明:常青藤茎段培养最适宜的激素为NAA0.2 mg?L-1,在此浓度下,茎段保留叶的大小在3/4~1 叶片,培养时间为15~20 d为宜。
- Abstract:
- In order to explore the suitable medium for rapid propagation of ?Hedera nepalensis ?var. sinensis under common conditions,and application in production of plants and flowers, taking stem segments as explants and 1/2 MS+vermiculite medium without sugar as basic medium,the effect of different concentrations and combination of hormones on the root growth of ?Hedera nepalensis var. sinensis was studied.The results showed that the suitable hormone for stem culture was NAA0.2 mg?L-1 ?and the leaf with 3/4~1 area culturing for 15~20 d was the best.
参考文献/References:
[1]张存旭,杨锋利,袁秀平.常春藤离体快繁技术[J].浙江林学院学报,2005,22(2):241-245.[2]王文斗,那冬晨,赵浩华.常春藤无土栽培试验[J].北方园艺,2009(12):187-188.[3]赵江雷,李显石.植物无糖组织培养技术[J].吉林蔬菜,2007(6):14-15.[4]杨武振,王荔,侯典云,等.无糖组织培养技术研究进展[J].云南学业大学学报,2004,19(3):239-242.[5]管道平,杨其长,刘文科,等.植物光自养微繁技术研究进展[J].园艺学报,2006,33(3):680-686.[6]侯典云,王聪睿,胥华伟,等.植物无糖组培快繁技术的应用[J].安徽农学通报,2010,16(11):72,148.[7]王蒂.植物组织培养实验指导[M].北京:中国农业出版社,2008:2-3.[8]刘振祥,廖旭辉.植物组织培养[M].北京:化学工业出版社,2007:14-17,30-32.
备注/Memo
收稿日期:2014-12-09基金项目:长春师范大学大学生创业基金资助项目第一作者简介:高立宏(1975-),女,吉林省长春市人,在读博士, 讲师,从事植物及分子遗传研究。E-mail:gaolh320@nenu.edu.cn。
更新日期/Last Update:
2015-06-08