LIU Li-hua,GUAN Lei,WANG Shuang.Effect of Ethanol Extract of Chelidonium majus on Protective Enzyme Activity in Pieris rapae L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(01):63-65.[doi:10.11942/j.issn1002-2767.2015.01.0063]
白屈菜乙醇提取液对菜青虫体内保护酶活性的影响
- Title:
- Effect of Ethanol Extract of Chelidonium majus on Protective Enzyme Activity in Pieris rapae L.
- 文章编号:
- 1002-2767(2015)01-0063-03
- Keywords:
- Celandine majus; Pieris rapae; protective enzyme
- 分类号:
- S462.3
- 文献标志码:
- A
- 摘要:
- 为利用白屈菜开发新型的植物源农药,采用分光光度法测定了白屈菜乙醇提取液对菜青虫体内保护酶活性的变化。结果表明:用白屈菜乙醇提取液处理菜青虫后,其体内SOD、CAT和POD活性都呈现先升高后降低的趋势,其中SOD酶活力在16 h达到最大,而CAT、POD都在24 h达到高峰,说明菜青虫在抵御逆境胁迫过程中,SOD是首先发生作用的保护酶,CAT和POD是继SOD之后发生作用的酶系。处理36 h前,3种保护酶处理组均高于对照组,48 h后,均低于对照组。说明,白屈菜乙醇提取液能有效干扰昆虫保护酶系,扰乱其正常的生理代谢,从而起到防治效果。
- Abstract:
- In order to develop new botanical pesticides by Celandine majus,Spectrophotometry was used to determine the change of ethanol extract of Chelidonium majus on protective enzyme activity of Pieris rapae L..The results showed that the SOD,CAT and POD activity were presented increased and then decreased after ethanol extract treating,which SOD enzyme activity reached maximum for 16 h,CAT and POD reached for 24 h. It indicated that in the process of withstand environmental stress for Pieris rapae L.,SOD was a protective enzyme which worked first,following with CAT and POD.Before 36 h,three protective enzyme treatments were higher than those in the control,after 48 h,they were lower than the control.The results showed that,ethanol extract of Chelidonium majus could effectively interfere insect protection enzymes,disrupt the normal physiological metabolism,so as to achieve to control.
参考文献/References:
[1] 陈多娇,谭大海,高雪,等.白屈菜乙醇提取物理化性质及驱虫性研究[J].北方园艺,2010(10):52-54.
[2] 张宏浩.白屈菜活性物质提取工艺及杀虫活性的研究[D].哈尔滨: 东北林业大学,2007.
[3] 张仙红,王宏民,李文英,等.菜青虫感染玫烟色拟青霉后血淋巴蛋白质含量及几种保护酶活力的变化[J].昆虫学报,2006,49(2):230-234.
[4] 李周直,沈惠娟,蒋巧根,等.几种昆虫体内保护酶系统活力的研究[J].昆虫学报,1994,4(37):399-403.
[5] 刘奇志,曹海锋,王玉柱,等.昆虫线虫抑制植物线虫的机理研究进展[J].植物保护,2006,32(6):13-17.
[6] Perry C A,Dwyer J,Gouris R R,Health effects salicylates in food and drug[J].Nutrient Rev,1996,54(8):225-240.
[7] 张志良.植物生理学实验指导[M].3版.北京:高等教育出版社,2003.
[8] Chance B,Maehly A C.Assay of catalases and peroxidases[J].Methods Enzymol,1955,11(2):764-775.
[9] 朱建兰,王国利,刘谨,等.四脊裸胞壳Dh菌株侵染对钩麦蛾幼虫体内保护酶的影响[J].草地学报,2008,16(2):121-125.
[10] 王楠,张志春,王满囷,等.腐胺对小菜蛾幼虫生长及保护酶活力的影响[J].昆虫知识,2008,45(4):573-576.
[11] 陈常理,韦茂兔,李明江,等.温度对B型烟粉虱存活率和保护酶系的影响[J].浙江农业科学,2010(4):853-857.
[12] 冯宏祖,刘映红,何林,等.阿维菌素和温度胁迫对朱砂叶螨自由基及保护酶活性的影响[J].植物保护学报,2008,35(6):530-536.
[13] 张奎花,钱秀娟,姬国红,等.异小杆线虫对菜青虫体内保护酶活力的影响[J].甘肃农业大学学报,2012,2(47): 58-61.
[14] 姬国红,钱秀娟,刘长仲,等.夜蛾斯氏线虫对菜青虫几种保护酶活力的影响[J].植物保护,2009,35(4) :66-69.
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备注/Memo
收稿日期:2014-07-12
基金项目:吉林省科技厅资助项目(20130101100JC);吉林省教育厅资助项目(2013493)
第一作者简介:刘丽华(1972-),女,吉林省通化市人,博士,副教授,从事动物资源与功能基因组研究。E-mail:liulihua209@163.com。