WANG Jia-min,PING Ling,XU Shui-lin,et al.Microcarrier Culture Technique and Its Applicationin Vaccine Production[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(12):146-149.
微载体培养技术及其在疫苗生产中的应用
- Title:
- Microcarrier Culture Technique and Its Applicationin Vaccine Production
- 文章编号:
- 1002-2767（2014）12-0146-04
- Keywords:
- microcarrier; cell culture; vaccine
- 分类号:
- R318.08
- 文献标志码:
- A
- 摘要:
- 微载体培养技术是一种新兴的细胞大规模培养技术，是目前贴壁依赖型细胞大规模培养的重要方法，其兼具贴壁培养和悬浮培养的优势，温度、溶氧浓度和pH等培养条件易控制，培养过程自动化、系统化，不易被污染。现就微载体培养技术的发展及其在疫苗生产中的应用进行了综述，并提出了动物细胞微载体大规模培养技术的发展方向。
- Abstract:
- Microcarrier cell culture technique is a new cell culture technology,which is primary method of large-scale culture of adherent-dependent cell currently.It possesses both adherent and suspension culture advantages，which is easy to control culture conditions such as temperature,oxygen concentration,pH and so on,and its culturing process is systematic,automated and hard to be contaminated.The development and application in vaccine production of microcarrier culture technology were summarized,and the development of large-scale animal cell microcarrier culture technique was proposed.
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备注/Memo
基金项目：教育部长江学者和创新团队发展计划资助项目（IRT13091）；甘肃省科技计划资助项目（1203FKDA030、2013GS08044）；兰州市科技计划资助项目（2013-4-101）