[1]苗则彦,白元俊,李 颖,等.沈阳市新民地区番茄黄化曲叶病毒分子检测[J].黑龙江农业科学,2014,(12):77-78.
　MIAO Ze-yan,BAI Yuan-jun,LI Ying,et al.Molecular Identification of Tomato Yellow Leaf Curl Virus from Xinmin Area of Liaoning Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(12):77-78.
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沈阳市新民地区番茄黄化曲叶病毒分子检测

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年12 页码: 77-78 栏目: 出版日期: 2014-12-10
Title:
Molecular Identification of Tomato Yellow Leaf Curl Virus from Xinmin Area of Liaoning Province
文章编号:
1002-2767（2014）12-0077-02
作者:
苗则彦; 白元俊; 李 颖; 赵 杨
辽宁省农业科学院 辽宁省农作物有害生物控制重点实验室，辽宁 沈阳 110161
Author(s):
MIAO Ze-yan; BAI Yuan-jun; LI Ying; ZHAO Yang
Liaoning Academy of Agricultural Sciences,Liaoning Key Laboratory of Crop Pest Management,Shenyang,Liaoning 110161
关键词:
番茄; 番茄黄化曲叶病毒病; 分子检测
Keywords:
tomato; tomato yellow leaf curl virus; molecular identification
分类号:
S436.412.1
文献标志码:
A
摘要:
为了鉴定并防治番茄黄化曲叶病毒病，采用分子生物学方法对沈阳新民地区3个番茄植株样品进行检测，对PCR产物中的特异性片段进行回收、克隆、测序。结果表明：3个番茄植株样品均感染番茄黄化曲叶病毒病，说明沈阳市新民地区已有番茄黄化曲叶病毒病发生且PCR技术可以快速、准确、高效地完成番茄黄化曲叶病毒病的检测
Abstract:
In order to authenticate and control tomato yellow leaf curl virus,3 tomato samples were detected through molecular identification of tomato yellow leaf curl virus(TYLCV)from Xinmin area of Liaoning province by PCR reaction,and then the generated specific segments were collected,cloned and sequenced.The results of Blast showed that the nucleotide sequence of 3 samples had high identity from TYLCV.PCR detection for TYLCV was high sensitive，high efficient and with high specificity.

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备注/Memo

基金项目：辽宁省农业综合开发省级重点科技推广资助项目(SNF-2014-A-01)

第一作者简介：苗则彦（1968-），女，辽宁省沈阳市人，博士，研究员，从事蔬菜病害防治研究。E-mail：mzyln@aliyun.com。

更新日期/Last Update: 2014-12-24