[1]张崎峰,靳学慧,蔡鑫鑫,等.黑龙江省稻瘟病菌无毒基因的检测[J].黑龙江农业科学,2014,(12):70-73.
　ZHANG Qi-feng,JIN Xue-hui,CAI Xin-xin,et al.Detection on Avirulence Gene of Rice Blast in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(12):70-73.
点击复制

黑龙江省稻瘟病菌无毒基因的检测

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年12 页码: 70-73 栏目: 出版日期: 2014-12-10
Title:
Detection on Avirulence Gene of Rice Blast in Heilongjiang Province
文章编号:
1002-2767（2014）12-0070-04
作者:
张崎峰1; 靳学慧2; 蔡鑫鑫1; 李金良1; 陈海军1
1.黑龙江省农业科学院 黑河分院，黑龙江 黑河164300；
2.黑龙江八一农垦大学，黑龙江 大庆 163319
Author(s):
ZHANG Qi-feng1; JIN Xue-hui2; CAI Xin-xin1; LI Jin-liang1; CHEN Hai-jun1
1.Heihe Branch of Heilongjiang Acadency of Agriculture Sciences,Heihe,Heilongjiang 164300;
2.Heilongjiang Bayi Agriculture University,Daqing,Heilongjiang 163319
关键词:
黑龙江省; 稻瘟病菌; 无毒基因; 分子标记
Keywords:
Heilongjiang province; Magnaporthe grisea; avirulent gene; molecular marker
分类号:
S435.111.4+1
文献标志码:
A
摘要:
为了探索黑龙江省稻瘟病菌无毒基因的分布情况，通过分子标记法对黑龙江省19个地区的204个稻瘟病菌的菌株进行PCR扩增，检测是否含有无毒基因Avr-pita、AVR-CO39、PWL2和PWL3。结果表明：各基因的出现频率差异较大，PWL2的出现频率较大，为52.94%，PWL3最低，出现频率为0，Avr-pita和AVR-CO39的出现频率分别为39.22%和37.75%。黑龙江省稻瘟病菌的群体结构复杂，在不同地区甚至相同地区的菌株中无毒基因的存在情况都有明显的差异。
Abstract:
In order to explore the distribution of rice blast fungus avirulent genes in Heilongjiang province,204 strains of Magnaporthe grisea were amplified by PCR through molecular marker method in 19 regions of Heilongjiang province isolates,the presence of avirulence genes Avr-pita,AVR-CO39,PWL2 and PWL3 was detected.The results showed that the gene frequency had larger difference,the frequency of PWL2 was 52.94% which was the highest,PWL3 was the lowest,the frequency was 0,Avr-pita and AVR-CO39 frequency were 39.22% and 37.75%,respectively.The population structure of rice blast in Heilongjiang province in the presence was complex,avirulence genes in different regions and even in the same region strains had obvious differences.

参考文献/References:

[1] 靳学慧 , 马汇泉 . 农业植物病理学 [M]. 赤峰：内蒙古科学技术出版社， 1999.

[2]李祥晓,王倩,罗生香，等.黑龙江省稻瘟病菌无毒基因分析及抗病种质资源筛选[J].作物学报,2012,38（12）:2192-2197.

[3]王世维,郑文静,赵家铭,等.辽宁省稻瘟病菌无毒基因型鉴定及分析[J].中国农业科学,2014,47(3):462-472.

[4]雷财林,凌忠专,王久林,等.北方稻区稻瘟病菌生理小种变化与抗病育种策略[J].作物杂志,2000(3):14-15.

[5]李亚,刘二明,戴良英,等.湖南稻瘟病菌群体遗传多样性与病菌致病型的关系[J].中国水稻科学,2007,21(3):304-308.

[6]林代福.单孢分离的眉毛挑针法[J].山地农业生物学报，2003，22（5）：462-463.

[7]He Y Q.An improved protocol for fungal DNA prepara-tion[J].Mycosystema,2000,19(3)：434.

[8]张崎峰，靳学慧，蔡鑫鑫,等.用SSR标记法对黑龙江省稻瘟病菌无毒基因的检测[J].中国农学通报，2010,26（10）:250-254.

[9]Valent B，Farrall L,Chumley F G.Magrroporthe grisea gene for pathogenicity and virulenc identified through a series of backcrosses[J].Genetics，1991，127：87-101.

[10]陆凡.江苏省稻瘟病菌遗传多样性研究[D].南京：南京农业大学，2000.

[11]De Wit P J G M.Molecnlar cliaracterization of gene for gene systems in plant-fungus interactions and the application of avirulence genes in control of plant patliogens[J].Annual Review of Phytopathology，1992，30：391-418.

[12]Dioh W，Tharreau D，Notteghem J L，et al.Mapping of avirulence genes In the Rice Blast Fungus，Magnaporthe griseawith RFLP and RAPD Markers[J].Molecular Plant-Microbe Interact，2000，13：217-227.

[13]Silue D,Notteghem J L,Tharreau D.Evidence of a gene-for-gene relationship in the Oryza saliva-Magnaporthe griseapathosystem[J].Phytopathology,1992a，82：577-580.


相似文献/References:

[1]赵雪虹.黑龙江省农村城镇化发展的金融支持模式研究[J].黑龙江农业科学,2014,(07):128.
　ZHAO Xue-hong.Research on the Financial Support of Rural Urbanization Development of Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(12):128.
[2]张 鹍,宋德禄,段亚东.黑龙江省黑穗醋栗的研究进展[J].黑龙江农业科学,2014,(05):147.
　ZHANG Kun,SONG De-lu,DUAN Ya-dong. Research Progress of Ribes nigrum in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(12):147.
[3]严洪冬,焦少杰,王黎明,等.黑龙江省甜高粱种质资源鉴定与评价[J].黑龙江农业科学,2013,(03):3.
　YAN Hong-dong,JIAO Shao-jie,WANG Li-ming,et al.Identification and Evaluation of Sweet Sorghum Germplasm Resources of Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(12):3.
[4]马金丰,李延东,王绍滨,等.谷子新品种龙谷34选育及栽培技术[J].黑龙江农业科学,2013,(01):148.
[5]刘海荣,杨晓华,郭金玲.黑龙江省地产果品销售现状及促销策略[J].黑龙江农业科学,2014,(03):120.
　LIU Hai rong,YANG Xiao hua,GUO Jin ling.Sales Status and Promotion Strategy of Local Fruits in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(12):120.
[6]孙海正.水稻覆膜节水增温高产栽培技术研究[J].黑龙江农业科学,2012,(01):28.
　SUN Hai-zheng.Study on Filming High Yield Cultivation Technique of Rice to Save Water and Increase Temperature[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(12):28.
[7]刘淑芬,来永才,宫秀杰.黑龙江省农民专业合作社存在问题及发展对策[J].黑龙江农业科学,2012,(01):114.
　LIU Shu-fen,LAI Yong-cai,GONG Xiu-jie.Problems and Countermeasures of Farmers SPecialized CooPerative in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(12):114.
[8]刘 成,张金成.建三江管理局水稻品种对稻瘟病的抗性分析[J].黑龙江农业科学,2012,(03):68.
　LIU Cheng,ZHANG Jin-cheng.Identification and Evaluation of Resistance of Rice to Magnaporthe grisea in Jiansanjiang Branch of HeilongjiangLand Reclamation General Bureau[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(12):68.
[9]罗新兰,芦 涛,姜丽霞,等.黑龙江省土壤水分分区与预报[J].黑龙江农业科学,2012,(06):40.
　LUO Xin-lan,LU Tao,JIANG Li-xia,et al.Partitioning and Prediction of Soil Moisturein Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(12):40.
[10]杨帆.基于农户信贷需求的视角创新黑龙江省农村金融产品与服务方式[J].黑龙江农业科学,2012,(05):132.
　YANG Fan.Innovation of Rural Financial Products and Service Mode in Heilongjiang Province Based on View of Farmer Credit Demand[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(12):132.

备注/Memo

基金项目：黑龙江省科技攻关资助项目（GB06B105-2）；黑龙江省农垦总局科技攻关资助项目（HNKXIV-01-04-02）

第一作者简介：张崎峰（1983-），男，黑龙江省鹤岗市人，硕士，助理研究员，从事植物病理学研究和玉米抗病育种工作。E-mail：hhzqf83@163.com。
通讯作者：靳学慧（1962-），男，教授，硕士研究生导师，从事植物病理学的教学与科研工作。E-mail：jxhbyndzky@yahoo.com.cn。

更新日期/Last Update: 2014-12-24