[1]杨晓坡,刘 冰,唐云轩,等.水稻线粒体基因组水平上的蛋白质编码基因克隆方法研究[J].黑龙江农业科学,2014,(12):9-14.
　YANG Xiao-po,LIU Bing,TANG Yun-xuan,et al.Genome-Wide Cloning of Protein-Coding Genesin the Rice Mitochondrial Genome[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(12):9-14.
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水稻线粒体基因组水平上的蛋白质编码基因克隆方法研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年12 页码: 9-14 栏目: 出版日期: 2014-12-10
Title:
Genome-Wide Cloning of Protein-Coding Genesin the Rice Mitochondrial Genome
文章编号:
1002-2767（2014）12-0009-06
作者:
杨晓坡1; 2; 刘 冰1; 唐云轩1; 金雨熙1; 石 珍1; 杜晓丽1; 宋 丽1
1.赤峰学院 生命科学学院，内蒙古 赤峰 024000；
2.山西大学 科学技术哲学研究中心，山西 太原 003006
Author(s):
YANG Xiao-po1; 2; LIU Bing1; TANG Yun-xuan1; JIN Yu-xi1; SHI Zhen1; DU Xiao-li1; SONG Li1
1.School of Life Science,Chifeng University,Chifeng,Inner Mongolia 024000;
2.Research Center for Philosophy of Science and Technology，Shanxi University，Taiyuan,Shanxi 030006
关键词:
水稻; 线粒体DNA; 蛋白质编码基因; 基因克隆
Keywords:
rice; mitochondrial DNA/mtDNA; protein-coding gene; gene cloning
分类号:
S511
文献标志码:
A
摘要:
为探索适用于水稻线粒体基因的克隆方法，以水稻线粒体基因ccmB、nad9为例，分别选用3种不同的方法进行克隆。在对水稻线粒体基因组蛋白质编码基因进行比对分析的基础上，探讨了各种克隆方法的优劣及适用范围。结果表明：常规方法适用于Ⅰ类水稻线粒体基因的克隆，RT-PCR法适用于Ⅱ类水稻线粒体基因的克隆，简易方法只适用于Ⅲ类水稻线粒体基因的克隆。
Abstract:
In order to explore cloning method suitable for the mitochondrial gene,taking rice mitochondrial gene ccmB and nad9 which are protein-coding genes as example,several methods were used for cloning.The results showed that the application scopes of different methods were discussed based on sequence analysis of mitochondrial protein-coding genes.The conventional method,RT-PCR method and the simple method were suitable forⅠ,Ⅱand Ⅲ genes respectively.

参考文献/References:

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备注/Memo

基金项目：内蒙古自治区教育厅高等学校科学研究资助项目（NJSY11219）；国家自然科学基金资助项目（31360572）

第一作者简介：杨晓坡（1979-），男，内蒙古自治区赤峰市人，博士，讲师，从事植物基因工程与科技哲学研究。E-mail：yangxp@hotmail.com。

更新日期/Last Update: 2014-12-24