[1]黎瑞源,石桃雄,刘筱嘉,等.荞麦分子遗传学研究进展[J].黑龙江农业科学,2014,(11):151-156.
　LI Rui-yuan,SHI Tao-xiong,LIU Xiao-jia,et al.Progress on Molecular Genetics of Fagopyrum Miller[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(11):151-156.
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荞麦分子遗传学研究进展

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年11 页码: 151-156 栏目: 出版日期: 2014-11-10
Title:
Progress on Molecular Genetics of Fagopyrum Miller
文章编号:
1002-2767（2014）11-0151-06
作者:
黎瑞源1; 石桃雄2; 刘筱嘉3; 陈庆富2
1.贵州师范大学 贵州省信息与计算机科学重点实验室，贵州 贵阳 550001；2.贵州师范大学 荞麦产业技术研究中心，贵州 贵阳 550001；3.番禺出入境检验检疫局，广州 番禺 510006
Author(s):
LI Rui-yuan1; SHI Tao-xiong2; LIU Xiao-jia3; CHEN Qing-fu2
1.Research Center of Buckwheat Industry Technology,Guizhou Normal University,Guiyang，Guiyang 550001;2.Key Laboratory of Information and Computing Science Guizhou Province,Guizhou Normal University,Guiyang，Guizhou 550001;3.Panyu Entryexit Inspection and Quarantine,Panyu,Guangzhou 510006
关键词:
荞麦; 分子标记; 遗传图谱; 基因克隆
Keywords:
Fagopyrum Miller; molecular marker; gene map; gene cloning
分类号:
S512
文献标志码:
A
摘要:
近年来生物技术使荞麦的分子遗传学研究取得了长足的进步，为进一步对荞麦分子遗传学进行研究，从种间亲缘关系、种质资源遗传多性性、遗传图谱构建、农艺性状及相关基因定位、基因克隆及功能研究、基因组测序等6个方面对荞麦分子遗传学研究进展进行了综述，提出了荞麦分子遗传研究中存在的问题，并对其发展趋势进行了展望。
Abstract:
In recent years,the developing and perfecting biotechnology make certain research progress in buckwheat,for the further study on molecular genetics of Fagopyrum Miller,recent advances of interspecific relationship,genetic diversity,genetic map construction,gene cloning and function,and genome sequencing were reviewed,the problems were proposed and the preliminary discussion on development trend was made. 

参考文献/References:

[1]Chen Q F.A study of resources of Fagopyrum(Polygonaceae)native to China[J].Botanical Journal Linnean Society,1999a,130:54-65.
 [2]Chen Q F.Wide hybridization among Fagopyrum(Polygonaceae)species native to China[J].Botanical Journal Linnean Society,1999b,131:177-185.
[3]陈庆富.荞麦属植物科学[M].北京：科学出版社,2012. [
4]任翠娟,陈庆富.荞麦属(Fagopyrum Mill)植物资源的RAPD研究\[J\].种子,2009,28(11):37-44.
[5]Tsuji K,Ohnishi O.Phylogenetic relationships among wild and cultivated tartary buckwheat(Fagopyrum tataricum Gaertn.)populations revealed by AFLP analy ses[J].Genes and Genetic Systems,2001,76(1):47-52.
 [6]Sharma T R,Jana S.Species relationships in Fagopyrum revealed by PCRbased DNA fingerprinting[J].Theoretical and Applied Genetics,2002,105(2-3)：306-312.
[7]Konishi T,Iwata H,Yashiro K,et al.Development and characterization of microsatellite markers for common buckwheat[J].Breeding Science,2006,56:277-285.
 [8]王莉花,殷富有,刘继梅,等.利用RAPD分析云南野生荞麦资源的多样性和亲缘关系[J].分子植物育种,2004,2(6):807-815.
 [9]杨小艳,陈惠,邵继荣,等.川西北荞麦种间亲缘关系初步研究[J].西北植物学报,2007,27(9):1752-1758.
[10]Steward AN.The polygoneas of eastern Asia[J].Contrib.Gray Herb.Harv.Univ.,1930,88:1-129.[11]]侯雅君,张宗文,吴斌,等.苦荞种质资源AFLP标记遗传多样性分析[J].中国农业科学,2009,42(12):4166-4174.
 [12]Senthikumaran R,Bisht I S,Bhat K V.Diversity in buckwheat(Fagopyrum spp.)landrace populations from north western Indian Himalayas[J].Genetic Resources and Crop Evolution,2008,55:287-302.
[13]张春平,何平,胡世俊,等.药用金荞麦遗传多样性的RAPD分析[J].中国中药杂志，2009,34(6):660-663.
[14]赵丽娟,张宗文,黎裕,等.苦荞种质资源遗传多样性的ISSR分析[J].植物遗传资源学报,2006,7(2):159-164.
[15]高帆，张宗文，吴斌.中国苦荞SSR分子标记体系构建及其在遗传多样性分析中的应用[J].中国农业科学,2012,45(6):1042-1053.
[16]Kump B,Javomikb.Genetic diversity and relationships among cultivated and wild accessions of tartary buckw heat(Fagopyrum tataricum Gaertn.)as revealed by RAPD markers[J].Genetic Resources and Crop Evolution,2002,49(6):565-572.
 [17]谭萍,王玉株,李红宁,等.十种栽培苦荞麦的随机扩增多态性DNA(RAPD)研究[J].种子,2006,25(7):46-49.
 [18]邓林琼，张奎，黄凯丰，等.甜荞和苦荞品种遗传多样性的RAPD分析[J].安徽农业科学,2011,39(15):88958898.
 [19]Zhang Z W，Zhao L J，Ramantatha V,et al.Assessment of genetic diversity of tartary buckwheat colledtion in china using AFLP markers[A].In:Chai Y,Zhang ZW eds.Proceedings of the 10th International Symposium on Buckwheat,Advances in Buckwheat Research[C].Yangling：Northwest A and F University Press,2007：68-75.
 [20]莫日更朝格图,王鹏科,高金锋,等.苦荞地方种质资源的遗传多样性分析[J].西北植物学报,2010,30(2):255-261.
[21]许瑾,周小梅,范玲娟,等.荞麦RA PD指纹图谱的建立及在品种鉴定中的应用[J].山西大学学报：自然科学版,2006,29(2):194-197.
 [22]Wang Z H，Zhan G Z，Lin R F.Seed protein diversity of buckwheat in China.Current advances in buckwheat research[M].Japan:Shinshu University Press,1995：411-417.
[23]张春平,何平,何俊星,等.ISSR分子标记对金荞麦8个野生居群的遗传多样性分析[J].中草药,2010,41(9):1519-1522.
[24]Ohnishi O.Nonshattering habit gene(sht),chlorophylldeficient and other detrimental genes concealed in natural populations of the wild ancestor of common buckwheat[J].Fagopyrum,1999,16:23-28.
[25]Yasuo Y,Wang Y,Ohnishi O,et al.Amplied fragment length polymorphism linkage analysis of common buckwheat(Fagopyrum esculentum)and its wild selfpollinated relativeFagopyrum homotropicum[J].Genome,2004,47:345-351.
 [26]Konishi T,Ohnishi O.A linkage map of common buckwheat based on microsatellite and AFLP markers[J].Fagopyrum,2006,23:1-6.
 [27]Pan S J,Chen Q F.Genetic mapping of common buckwheat using DNA,protein and morphological markers[J].Hereditas,2010,147:27-33.
[28]岳鹏.普通荞麦自交可育F2遗传群体构建及其遗传标记图谱研究[D].贵阳：贵州师范大学，2012.
 
[29]Li F L,Zeller F J,Huang K F,et al.Improvement of fluorescent chromosome in situ PCR and its application in the phylogeny of the genus Fagopyrum Mill.using nuclear genes of chloroplast origin(cpDNA)[J].Plant Systematics and Evolution,2013,299(9):1679-1691.
[30]王耀文,夏楠,韩瑞霞,等.苦荞SSR-PCR反应体系的优化及引物筛选[J].贵州农业科学,2011,39(4):4-8.
[31]Matsui K,Tetsuka T,Hara T.Two independent gene loci controlling nonbrittle pedicel in buckwheat[J].Euphytica,2003,134:203-208.
[32]Wang Y J,Rachael S G,Clayton C.Inheritance of seed shattering in interspecific hybrids between Fagopyrum esculentum and F.homotropicum[J].Crop Science,2005,45:693-697.[33]岳鹏,黄凯丰,陈庆富.普通荞麦落粒性、尖果和红色茎秆的遗传规律研究[J].河南农业科学,2012,41(1):28-31.
[34]Yasuo Y,Wang Y,Ohnishi O,et al.Amplified fragment length polymorphism linkage analysis of common buckwheat(Fagopyrum esculentum)and its wild selfpollinated relative Fagopyrum homotropicum[J].Genome,2004,47:345-351.
[35]Sharma K D,Boyes,J W.Modified incompatibility of buckwheat following irradiation[J].Canadian Journal Botany,1961,39:1241-1246.
[36]Woo S H,Adachi T,Jong S K,et al.Inheritance of selfincompatibility and flower morphology in an interspecific buckwheat hybrid[J].Canadian Journal Plant Science,1999,79:483-490.
[37]Chen Q F.A Study of isozyme,and interspecific hybridization on bigachene group of buckwheat species(Fagopyrum,Polygonaceae)[J].Crop Sciences,2004,44:1511-1518.
[38]Yasui Y,Mori M,Aii J,et al.SLocus Early Flowering 3 is exclusively present in the genomes of shortstyled buckwheat plants that exhibit heteromorphic selfincompatibility[J].PLOS ONE,2012,7(2):e31264.
[39]高帆,张宗文,李艳琴,等.苦荞中查尔酮合成酶基因(CHS)的克隆.[J].中国农学通报,2011,27(21):207-214.
[40]Liu K,Hu Y H,Wang G,et al.Cloning and analysis of a chalone synthase gene from Fagopyrum tataricum[J].Agricultural Science and Technology,2012,13(4):708-710,726.
[41]李成磊,张晓伟,吴琦,等.甜荞查尔酮合酶基因Chs的克隆及序列分析[J].广西植物,2011,31(3)：383-387.
[42]蒙华,李成磊,吴琦,等.金荞麦查尔酮合成酶基因CHS的克隆及序列分析[J].草业科学,2010,19(3):162-169.
[43]张艳,柴岩,冯佰利,等.苦荞和甜荞查尔酮合成酶基因的克隆及序列比较[J].西北植物学报,2008,28(3):447-451.
[44]马婧,祝钦泷,郭铁英,等.金荞麦MYB转录因子基因FdMYBP1的克隆及分子特征分析[J].中国中药杂志(A1),2009,34(17):2155-2159.
[45]雒晓鹏,白悦辰,高飞,等.金荞麦查尔酮异构酶的基因克隆及其花期表达与黄酮量分析[J].中草药,2013,40(11):1481-1485.
[46]马婧，王斌，代银，等.金荞麦无色花色素还原酶基因FdLAR的克隆和表达分析[J].药学学报,2012,47(7):953-961.
[47]Ufisu A,Kondo Y.Identification of a mjor allergen of buckwheat seeds by immunoblotting methods[J].Allergy Clinlmmunol News,1994,6:151-155.
[48Kondo Y,Urisa A,Wada E,et al.Allergen analysis of buckwheat by theimmunoblotting method[J].Arerugi,1993,42(2):142-148.
[49Nair A,Adachi T.Immunodetection and characterization of allergenicproteins in common buckwheat(Fagopyrum esculentum)[J].Plant Biotechnology,1999,16:219-224.
[50]Wang Z H,Wang L,Chang W J.Cloning,expression,and identification of immunological activity of an allergenic protein in tartary buckwheat[J].Bioscience,Biotechnology and Biochemistry,2006,70:1195-1199.
[51]Wang Z H,Zhang Z,Wieslander G,et al.Purification and some properties of the protein with 22 kD in tartary buckwheat [J].Fagopyrum,2000,17:441-444.
[52]赵小珍,张政,景巍,等.苦荞麦主要过敏蛋白N端基因片段的克隆及序列分析[J].食品科学,2006,27(10):41-44.
[53]王岚,李玉英,蔡桂红,等.重组苦荞麦过敏蛋白TBa的原核表达及其免疫活性鉴定[J].中国生物化学与分子生物学报,2006,22(4):308-312.
[54]Logacheva M D,Kasianov A S,Vinogradov D V,et al.Denovo sequencing and characterization of floral transcriptome in two species of buckwheat(Fagopyrum)[J].BMC Genomics,2011,12:30.[55]石桃雄,黎瑞源,郭菊卉,等.普通荞麦种子转录谱EST-SSR标记的开发[J].贵州农业科学,2014,42(3):1-5.

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备注/Memo

收稿日期：2014-06-26基金项目：国家自然科学基金资助项目（31460280)；贵州省科技厅科学技术基金资助项目（黔科合J字[2013]2213，黔科合JZ字[2014]2011号）；贵州师范大学博士基金资助项目（11904-05032130025）第一作者简介：黎瑞源（1980-），男，广西壮族自治区阳朔县人，博士，讲师，从事生物信息学研究。E-mail:liruiyuan@live.cn。通讯作者：石桃雄（1980-），女，副教授，从事植物遗传育种研究。E-mail:shitaoxiong@126.com。

更新日期/Last Update: 2014-12-12