JIANG Fang-yan,MA Jun,CHEN Yong-gan,et al.Research Progress on Active Ingredients of Ganoderma sp.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(08):137-142.
灵芝活性成分的研究进展
- Title:
- Research Progress on Active Ingredients of Ganoderma sp.
- 文章编号:
- 1002-2767(2014)08-0137-06
- Keywords:
- Ganoderma sp.; polysaccharide; triterpenoid; protein; bioactivity
- 分类号:
- Q936
- 文献标志码:
- A
- 摘要:
- 灵芝活性成分复杂,其主要的生理活性物质有多糖类、三萜类、免疫调节蛋白类、凝集素类、核苷类、甾醇类、生物碱类和微量元素等,具有抗肿瘤、抗氧化、调节免疫、降血糖、降血脂、抗病毒、消炎抗菌等功效,因此,对其活性成分的研究具有重要意义。现对灵芝的主要活性成分及其生物活性的国内外研究现状进行综述,为灵芝产品的进一步开发利用提供理论依据。
- Abstract:
- The active ingredients of Ganoderma sp.are very complex,the major physiologically active ingredients are polysaccharides,triterpenoids,immunomodulatory proteins,lectins,nucleosides,strols,alkaloids and trace elements,which have the effect of anti-tumor,anti-oxidation,immunomodulatory,hypoglycemic,hypolipidemic,anti-virus,anti-inflammatory and antibacterial,so the research of its active ingredients has inportant significance.The current research status on the major active ingredients of Ganoderma sp.and its bioactivity were reviewed both at home and abroad as to provide a theoretical basis for further development and utilization of Ganoderma sp.products.
参考文献/References:
[1] 李时珍.本草纲目(校点本)[M].北京:北京人民卫生出版社,1982.
[2]屈直,谢晴宜,马海霞.灵芝液体深层发酵技术研究进展[J].热带农业科学,2014,34(2):93-98.
[3]江艳,王浩,吕龙,等.灵芝孢子粉多糖Lzps-1的化学研究及其总多糖的抗肿瘤活性[J].药学学报,2005,40(4):347-350.
[4]Zhang H,Li W J,Xie M Y,et al.Structural characterization of a novel bioactive polysaccharide from Ganoderma atrum[J].Carbohydrate Polymers,2012,88(3):1047-1054.
[5]Han X Q,Tu P F,Han Q B,et al.Structural characterization and immuno-modulating activities of a polysaccharide from Ganoderma sinense[J].International Journal of Biological Macromolecules,2012,51:597-603.
[6]Zhang S S,Nie S P,Xie M Y,et al.Immunomodulatory effect of Ganoderma atrum polysaccharide on CT26 tumor-bearing mice[J].Food Chemistry,2013,136:1213-1219.
[7]游育红,林志彬.灵芝多糖肽对ECV304细胞氧化损伤的保护作用[J].中国药理学通报,2007,23(11):1510-1513.
[8]Shi M,Zhang Z Y,Yang Y N.Antioxidant and immunoregulatory activity of Ganoderma lucidum polysacch-aride(GLP)[J].Carbohydrate Polymers,2013,95:200-206.
[9]Meng G L,Zhu H Y,Yang S J,et al.Attenuating effects of Ganoderma lucidum polysaccharides on myocardial collagen cross-linking relates to advanced glycation end product and antioxidant enzymes in high-fat-diet and streptozotocin-induced diabetic rats[J].Carbohydr Polym,2011,84(1):180-185.
[10]吴锋,孟国梁,杨丽云,等.灵芝多糖预防大鼠动脉粥样硬化的实验研究[J].南通大学学报:医学版,2008,28(4):251-252.
[11]赵成萍,冯翠萍,常晓敏.灵芝多糖抑菌作用的研究[J].食用菌,2012(2):60-61.
[12]Fatmawati S,Shimizu K,Kondo R.Structure-activity relationships of ganoderma acids from Ganoderma lucidum as aldose reductase inhibitors[J].Bioorganic and Medicinal Chemistry Letters,2011,21:7295-7297.
[13]Tang W,Liu J W,Zhong J J.Ganoderic acid T from Ganoderma lucidum mycelia induces mitochondria mediated apoptosis in lung cancer cells[J].Life Sciences,2006,80:205-211.
[14]Liu RM,Zhong JJ.Ganoderic acid Mf and S induce mitochondria mediated apoptosis in human cervical carcinoma HeLa cells[J].Process Biochemistry,2011,18(5):349-355.
[15]周昌艳,唐庆九,林志彬,等.灵芝中有效成分灵芝酸的抑制肿瘤研究[J].菌物学报,2004,23(2):275-279.
[16]洪介民,黎庆梅.灵芝三萜对T淋巴细胞的活化作用[J].中药新药与临床药理,2007,18(4):283-285.
[17]Ko H H,H C F,Lin C N,et al.Antiinflammatory triterpenoids and steroids from Ganoderma lucidum and G.tsu-gae[J].Phytochemistry,2008,69:234-239.
[18]Li W J,Nie S P,Xie M Y,et al.Antimicrobial properties,antioxidant activity and cytotoxicity of ethanol-soluble acidic components from Ganoderma atrum[J].Food and Chemical Toxicology,2012,50:689-694.
[19]柳永,郭丽琼,王海燕,等.灵芝免疫调节蛋白基因的克隆和分析[J].热带作物学报,2006,27(1):54-58.
[20]Lin J W,Hao L X,Xu G X,et al.Molecular cloning and recombinant expression of a gene encoding a fungal immunomodulatory protein from Ganoderma lucidum in Pichia pastoris[J].World Journal Microbiol Biotechnol,2008,25(3):383-390.
[21]Peng Y F,Zhang L N.Characterization of a polysaccharide protein complex from Ganoderma tsugae mycelium by size-exclusion chromatography combined with laser light scattering[J].J Biochem Biophys Methods,2003,56:243-252.
[22]Ngai P H,Ng T B.A mushroom(Ganoderma capense)lectin with spectacular thermostability,potent mitogenic activity on splenocytes,and antiproliferative activity toward tumor cells[J].Biochem BiopHRes Co,2004,314:988-993.
[23]张春玉,黄国辉,林景卫,等.长白山药用真菌树舌灵芝凝集素的纯化及生化特性[J].中国生物工程杂志,2005,25(9):84-88.
[24]何云庆,李荣芷,蔡廷威,等.灵芝肽多糖的化学研究[J].中草药,1994,25(8):395-397.
[25]Zhang JS,Tang QJ,Fan H,et al.Activation of B lymphocytes by GLIS,a bioactive proteoglycan from Ganoderma lucidum[J].Life Sciences,2002,71:623-638.
[26]Lin J W,Hao L X,Xu G X,et al.Molecular cloning and recombinant expression of a gene encoding a fungal immunomodulatory protein from Ganoderma lucidum in Pichia pastoris[J].World Journal Microbiol Biotechnol,2008,25(3):383-390.
[27]Lin C H,Chang J G,Ko J L,et al.A new immunomodulatory protein from Ganoderma microsporum inhibits epidermal growth factor mediated migration and invasion in A549 lung cancer cells[J].Process Biochemistry,2010,45:1537-1542.
[28]Wang P H,Hsu C I,Tang S C,et al.Fungal immunomodulatory protein from Flammulina velutipes induces interferon-γ production through p38 mitogen-activated protein kinase signaling pathway[J].Agricultural and Food Chemistry,2004,52(9):2721-2725.
[29]林忠,白杰英,李彦舫.真菌免疫调节蛋白(FIP)结构与功能研究[J].辽宁师范大学学报,2006,29(1):84-87. [30]Ming Du,Lei Zhao,Guanghua Zhao,et al.Purification and characterization of a novel fungi Se-containing protein from Se-enriched Ganoderma Lucidum mushroom and its Se-dependent radical scavenging activity[J].European Food Research and Technology,2007,224(5):659-665.
[31]Liu J,Yang F,Ye L B,et al.Possible mode of action of anti-herpetic activities of a p roteoglycan isolated from the mycelia of Ganoderm alucidum in vitro[J].J Ethnopharm acol,2004,95:265-272.
[32]Wang X M,Yang M,Guan S H,et al.Quantitative determination of six major triterpenoids in Ganoderma lucidum and related species by high performance liquid chromatography[J].J Pharm Biomed Anal,2006,41(3):838-844.
[33]张圣龙,周靖,谢晓梅,等.不同品种灵芝中四种核苷类成分的含量比较[J].食用菌学报,2012,19(4):67-70.
[34]Liu YW,Gao JL,Guan J,et al.Evaluation of Antiproliferative Activities and Action Mechanisms of Extracts from Two Species of Ganoderma on Tumor Cell Lines[J].J Agric Food Chem,2009,57(8):3087-3093.
[35]何晋浙,赵培成,张安强,等.灵芝及其类似品中的20种微量元素的分析研究[J].药物分析杂志,2010,30(5):847-852.
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备注/Memo
收稿日期:2014-04-20
基金项目:海南省自然基金(313049);三亚市院地科技合作项目(2012YD12,2012YD09);琼州学院青年教师科研基金(QYQN201237,QYQN201235);海南省大学生创新训练资助项目(11223089)
第一作者简介:姜芳燕(1984-),女,浙江省衢州市人,博士,讲师,从事微生物的资源及其催化研究。E-mail:jiangfy2005@126.com。
通讯作者:王沛正(1972-),男,博士,副教授,从事教学和分子生物学研究。E-mail:kondywpz@126.com。