[1]刘亚莉,李运生,章美林,等.不同因素对安徽白山羊超数排卵的影响[J].黑龙江农业科学,2014,(06):77-81.
　LIU Yali,LI Yunsheng,ZHANG Meilin,et al.Effect of Different Factors on Superovulation of Anhui White Goat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(06):77-81.
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不同因素对安徽白山羊超数排卵的影响

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年06 页码: 77-81 栏目: 出版日期: 2014-06-10
Title:
Effect of Different Factors on Superovulation of Anhui White Goat
文章编号:
1002-2767（2014）06-0077-05
作者:
刘亚莉1; 李运生1; 2; 3; 章美林1; 4; 陈 真1; 詹 年1; 章孝荣1; 2; 3; 刘 亚1; 2; 3
1.安徽农业大学 动物科技学院，安徽 合肥 230036；2.安徽地方畜禽遗传资源保护与生物育种省级实验室，安徽 合肥 230036；3.安徽省羊繁育工程技术研究中心，安徽 合肥 230036；4.安徽省妇幼保健医院，安徽 合肥230000
Author(s):
LIU Yali1; LI Yunsheng1; 2; 3; ZHANG Meilin1; 4; CHEN Zhen1; ZHAN Nian1; ZHANG Xiaorong1; 2; 3; LIU Ya1; 2; 3
1.College of Animal Sciences and Technology,Anhui Agricultural University,Hefei,Anhui 230036;2.Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio Breeding,Hefei,Anhui 230036;3.Engineering Research Center of Reproduction and Breeding in Goat of Anhui Province,Hefei,Anhui 230036;4.Maternal and Child Health Hospital of Anhui Province,Hefei,Anhui 230000
关键词:
山羊; 超数排卵; CIDR; 季节; 妊娠
Keywords:
goat; superovulation; CIDR; season; pregnancy
分类号:
S827.36
文献标志码:
A
摘要:
为获得更多安徽白山羊受精卵，以开展转基因育种工作，研究了不同孕酮阴道栓来源、季节以及供体妊娠状态对安徽白山羊超数排卵效果的影响。结果表明：使用国产孕酮阴道栓（CIDR）对供体母羊进行同步发情处理，头均排卵数和回收受精卵数分别为15.0±7.62和14.6±7.88枚，受精率为97.3%，而应用进口孕酮阴道栓对供体山羊进行同步发情处理，头均排卵数和回收受精卵数分别为11.0±5.38和10.8±5.16枚，受精率为98.2%，两组间差异不显著（P＞0.05）；安徽白山羊在春、秋、冬3个季节超数排卵，头均回收卵子数分别为13.71±6.28、16.54±7.43和13.95±6.15枚，季节间差异不显著（p＞0.05）；妊娠母羊和未妊母羊头均排卵数分别为14和15.2枚，两者差异不显著（P＞0.05），但妊娠组回收卵子的受精率显著低于空怀组（P＜0.05）。
Abstract:
To obtain more oocytes from Anhui white goat for transgenic study,the effects of season,dornors’ pregnency and the source of CIDR on superovulation in Anhui white goat were investigated.The results showed that when synchronized with domestic CIDR,average number of ovulated oocytes and colleted fertilized oocytes per donor were 15.0±7.62 and 14.6±7.88 respectively,and fertilization rate was 97.3%,while synchronized with imported CIDR,average number of ovulated oocytes and colleted fertilized oocytes per donor were 11.0±5.38 and 10.8±5.16 respectively,and fertilization rate was 98.2%,there was no significant difference between the two groups(P＞0.05);Average number of collected oocytes per donor were 13.71±6.28,16.54±7.43 and 13.95±6.15 respectively when superoulated in spring,autumn and winter,there was no significant difference between any seasons(P＞0.05);Average number of ovulated oocytes in pregnant and nonpregnant dornors were 14.0 and 15.2 respectively,there was no significant difference between two groups,but the fertilization rate of oocytes collected from pregnant dorors was lower than that of non prenant dornors significantly(P＜0.05).

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备注/Memo

收稿日期：2014-02-26

基金项目：安徽省自主创新专项资助项目（12z010244）
第一作者简介：刘亚莉（1990-），女，安徽省灵璧县人，硕士，从事哺乳动物胚胎工程研究。通讯作者：刘亚（1972-），男，安徽亳州人，博士，副教授，从事动物繁殖技术研究。E-mail:liuya@ahau.edu.cn。

更新日期/Last Update: 2014-08-03