XIE Li-yang,KUANG Hua-qin,LAI Zhong-xiong,et al.Study on Adventitious Buds Induction and Elongation of Capsicum annuum L. Cotyledon[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(10):12-15.
辣椒子叶不定芽的诱导与伸长研究
- Title:
- Study on Adventitious Buds Induction and Elongation of Capsicum annuum L. Cotyledon
- 文章编号:
- 1002-2767(2013)10-0012-04
- Keywords:
- Capsicum annuum L.; cotyledon; adventitious buds induction; elongation
- 分类号:
- S641.3
- 文献标志码:
- A
- 摘要:
- 为了进一步研究辣椒子叶再生体系的影响因素,以辣椒王种子为试材,诱导无菌苗,取子叶作为外植体,探讨了不同浓度6-BA对辣椒子叶诱导不定芽的影响,并进行了不同浓度G3与6-BA组合对不定芽伸长效果的研究。结果表明:辣椒子叶在MB5+0.8 mg.L-1 IAA+8.0 mg.L-16-BA+4.0 mg.L-1AgNO3培养基上诱导不定芽效果最好;MB5+0.8 mg.L-1 IAA+5.0 mg.L-1 6-BA+2.0 mg.L-1 GA3+4.0 mg.L-1AgNO3培养基有利于不定芽的伸长。
- Abstract:
- In order to further study the influence factors of cotyledon regeneration system of in vitro Capsicum annuum L.,‘Lajiaowang’ seeds were used to induce in vitro plantlets(Capsicum annuum L.).The differences of adventitious buds induction were compared among the different concentrations of 6-BA.In the meanwhile,the effects of the different concentrations of GA3 and 6-BA combinations on elongation were analyzed.The results showed that the best medium for adventitious buds induction by hypocotyls was MB5+0.8 mg.L-1 IAA+8.0 mg.L-1 6-BA+4.0 mg.L-1 AgNO3.The suitable medium for the adventitious buds elongation was MB5+0.8 mg.L-1 IAA+5.0 mg.L-1 6-BA+2.0 mg.L-1 GA3+4.0 mg.L-1 AgNO3。
参考文献/References:
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备注/Memo
收稿日期:2013-04-08