SUNJing-liang,SUNSheng-xiang,LIUQiang-qiang,et al.EffectofZTontheGrowthof Chrysanthemum morifolium Ramat invitro[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(06):8-10.
ZT对地被菊品种——醉西施试管苗的影响
- Title:
- EffectofZTontheGrowthof Chrysanthemum morifolium Ramat invitro
- 文章编号:
- 1002-2767（2013）06-0008-03
- Keywords:
- Chrysanthemummorifolium Ramat; tissueculture; tubeseedlings
- 分类号:
- S688.4
- 文献标志码:
- A
- 摘要:
- 为解决醉西施在观赏栽培中植株较高、花朵较少的问题，以具有生长点的嫩茎为材料，应用植物组织培养方法，对试管苗进行了在培养基中加入ZT0.8mg.L-1和向已经栽培3年的试管苗上喷洒ZT1.0mg.L-1两种不同处理对醉西施试管苗的影响试验。结果表明：1/2MS+蔗糖10g.L-1+IAA0.2mg.L-1是嫩茎段生根培养和试管苗茎段生根继代培养的理想培养基；1/2MS+蔗糖10g.L-1+IAA0.2mg.L-1+ZT0.4mg.L-1是培养植株较矮、花朵多的醉西施试管苗的较理想培养基；向生长旺盛的试管苗喷洒ZT1.0mg.L-1会使试管苗出现植株较矮、花朵多的性状；试管苗移栽成活率为98%以上，移植成活率为100%；移植的试管苗形成了植株矮小、花朵数多、观赏效果好的性状。
- Abstract:
- Inordertosolvetheproblemthattheplantsarehigherandflowersarelessinornamentalcultivation,usingtissueculturetechnique,thetenderstemswithgrowingpointsof ChrysanthemummorifoliumRamatwereusedasmaterialstodotheresearch.TreatthetubeseedlingsseparatelybyaddingZT0.8mg.L-1 tomediumandsprayingZT1.0mg.L-1 totubeseedlingswhichhavebeencultivatedforthreeyears,andthenobservethedifferentinfluences.Theresultsdemonstratedthat1/2MS+sucrose10g.L-1+IAA0.2mg.L-1 wastheidealmediumforrootingandrootingsubcultureoftubeseedlings.Thebestmediumforcultivatingshorterplantsandmoreflowerswas1/2MS+sucrose10g.L-1+IAA0.2mg.L-1+ZT0.4mg.L-1.SprayingZT1.0mg.L-1 tothetubeseedlingswhichgrewvigorouslycouldleadtotheshorterplantsandmoreflowers.Thetransplantingsurvivalrateoftubeseedlingswasmorethan98%andthestableplantingsurvivalratewas100%.Colonizationofplantletsmaintainedthetraitsthattheshorterplantheightwere,muchmoreflowersandbetterornament.
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备注/Memo
收稿日期：2013-01-29
基金项目：辽宁省大学生创新创业训练资助项目（201303015011）;辽宁省普通高等教育本科教学改革研究资助项目（201203041-4）
第一作者简介：孙婧靓（1992-），女，辽宁省大连市人，在读学士，从事植物组织培养研究研究。
通讯作者：姜长阳（1953-）,男，辽宁省大连市人，学士，教授，从事植物技术研究。E-mail:changyangjiang@126.com。