LIANG Xiao-wen,DING Yun-hua,CHEN Min,et al.Study of Rapid Test Technology of Pesticide Residues in Vegetables[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(04):125-129.
蔬菜农药残留快速检测技术研究
- Title:
- Study of Rapid Test Technology of Pesticide Residues in Vegetables
- 文章编号:
- 1002-2767（2013）04-0125-05
- Keywords:
- vegetables; pesticide residue; inhibition rate of enzyme
- 分类号:
- TS255.7
- 文献标志码:
- A
- 摘要:
- 为了快速检测蔬菜中农药残留问题，应用农残速测仪，以绿健公司生产的试剂为检测试剂，对蔬菜中农药残留快速检测技术进行优化研究。结果表明：12种农药对乙酰胆碱酯酶的抑制率与农药浓度之间呈负相关，随着浓度的下降，酶抑制率也降低；有11种农药检出限低于国标的检测限，只有丁硫克百威比国标稍高；对酶、底物和显色剂的用量进行优化，酶加75 μL，底物和显色剂各加50 μL为最优组合。研究结果可以为基层开展农药残留检测提供技术支持。
- Abstract:
- For solving the question of rapid test of pesticide residues in vegetables,the apparatus of pesticide residues was applied,and the tested reagent made by the company of Lvjian was used to improve the rapid test technology of pesticide residues in vegetables.The results indicated that 12 kinds of pesticides influencing the inhibition of acetylcholinesterase activity correlated negatively with the concentration of parathion,that was to say the inhibition of ChE activity was decreasing with the concentration of parathion reduction.The detection limit of 11 kinds of pesticides were inferior to the standard of our country,but the detection limit of carbosulfan exceed the standard of our country.the dosage of enzyme,substrate and chromogenic reagent were optimized:enzyme adding 75 μL,substrate and chromogenic reagent adding 50 μL，respectively.The component was optimal.The study results could provide technical support for grass roots to carry out test of pesticide residues.
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备注/Memo
收稿日期：2013-01-29
基金项目：广东省科技计划资助项目（2011B030800021）；惠州市科技计划资助项目（2012P16）；惠城区科技计划资助项目（201106）
第一作者简介：梁晓文（1973-），女，山西省祁县人，工程师，从事农产品质量检测研究。E-mail：chenzg1973@126.com。