[1]唐文杰,王玉,周泉澄,等.马齿苋组培与耐盐筛选[J].黑龙江农业科学,2013,(03):7-9.
　TANG Wen-jie,WANG Yu,ZHOU Quan-cheng,et al.Tissue Culture and Salt Resistance Selection of Portulaca oleracea L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(03):7-9.
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马齿苋组培与耐盐筛选

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2013年03 页码: 7-9 栏目: 出版日期: 2013-03-30
Title:
Tissue Culture and Salt Resistance Selection of Portulaca oleracea L.
文章编号:
1002-2767（2013）03-0007-03
作者:
唐文杰; 王玉; 周泉澄; 张边江
南京晓庄学院 生物化工与环境工程学院，江苏 南京 211171
Author(s):
TANG Wen-jie; WANG Yu; ZHOU Quan-cheng; ZHANG Bian-jiang
Biochemical and Environmental Engineering School of Nanjing Xiaozhuang College,Nanjing,Jiangsu 211171
关键词:
马齿苋; 组织培养; 植株再生; 耐盐
Keywords:
Portulaca oleracea L.; tissue culture; plantlet regeneration; salt-tolerance
分类号:
S567.21
文献标志码:
A
摘要:
为了扩大耐盐植物资源，促进沿海滩涂植物资源利用，以马齿苋（Portulaca oleracea L.）的嫩叶和茎段为试验材料，研究了不同浓度激素和NaCl对愈伤组织生长与分化的影响。结果表明：MS+0.10 mg?L-1NAA时，马齿苋茎段生根率较高，生长健壮；MS+2.00 mg?L-16-BA是不定芽诱导的最适培养基；MS+2.00 mg?L-16-BA+1.00 mg?L-1 NAA是分化的最适培养基；在最适分化培养基中添加100 mmol?L-1NaCl，诱导产生的愈伤组织为5%，大部分愈伤组织生长受抑制，同时也说明马齿苋具有一定的耐盐性。
Abstract:
In order to expand salt-tolerant plants,promote the coastal beach plant resources utilization.With the stem and tender leaves of Portulaca oleracea L. as experimental material,the effects of different concentration of 6-BA and NAA and NaCl on growth and differentiation of callus were studied.The results showed that when MS+0.10 mg?L-1NAA,the rooting of stem cutting was relatively high.MS+2.00 mg?L-16-BA was the optimal medium for adventitious bud induction;MS+2.00 mg?L-16-BA+1.00 mg?L-1NAA was the optimal medium for differentiation.When stressed by 100 mmol?L-1NaCl on the optimal differentiation mediumn,the callus induce frequency was only 5% and the formation of callus was inhibited,it indicated that Portulaca oleracea L. had certain salt resistance.

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备注/Memo

收稿日期：2013-01-04

基金项目：江苏省大学生创新训练资助项目(2160081);江苏省教育厅高校自然基金资助项目(08 KJD180012)
第一作者简介：唐文杰(1989-),女,江苏省灌南县人,在读学士，从事作物生理研究。
通讯作者：张边江(1979-),男,江苏省东海县人,博士,副教授,从事作物生理研究。E-mail：zhangbjiang@yahoo.cn。

更新日期/Last Update: 2014-10-25