[1]马勇. 部分小麦种质中抗秆锈病基因的初步分子检测[J].黑龙江农业科学,2013,(01):7-10.
　MAYong.MolecularDetectionoftheResistanceGene Sr22toStemRustinWheatGermplasm[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):7-10.
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 部分小麦种质中抗秆锈病基因的初步分子检测

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2013年01 页码: 7-10 栏目: 出版日期: 2013-01-31
Title:
MolecularDetectionoftheResistanceGene Sr22toStemRustinWheatGermplasm
文章编号:
1002-2767（2013）01-0007-04
作者:
马勇
 黑龙江省农业科学院 克山分院，黑龙江 克山 161606
Author(s):
MAYong
KeshanBranchofHeilongjiangAcademyofAgriculturalSciences,Keshan,Heilongjiang161606
关键词:
Sr22;  ; 小麦秆锈病; Ug99; 分子检测
Keywords:
Sr22; wheatstemrust; Ug99; moleculardetection
分类号:
S512.1
文献标志码:
A
摘要:
 抗病基因Sr22不仅抗国内流行的主要小麦秆锈病生理小种，而且对新型秆锈菌生理小种Ug99（TTKSK）及其变异种TTKST和TTTKS具有很好的抗性。利用与抗病基因Sr22紧密连锁的SSR引物Xcfa2019，对从国内外搜集的抗Ug99的小麦种质以及黑龙江省主栽小麦品种进行SSR分子检测。结果表明：从国内外引进的58份抗Ug99小麦种质中未检测到抗病基因Sr22；黑龙江省18份主栽春小麦品种中有3份材料中含有抗病基因Sr22，占检测材料的4%。表明抗病基因Sr22在黑龙江省主栽小麦品种中占有一定的比例，今后要继续加强Sr22在小麦抗病育种中的应用。
Abstract:
Sr22isanimportantstemrustresistantgene,notonlyresistanttothedomesticmainstemrustraces,butalsotothenewvirulentpathogenUg99(TTKSK)anditsvariantsracesTTKSTandTTTKS.ThemicrosatellitemarkerscloselylinkedtoSr22werescreenedtobegenediagnoseforresistantgermplasmandvarieties.Sr22geneinwheatcultivarswasidentifiedbyusingaSSRmarkerXcfa2019,themarkerwithgoodrepeatabilityandhighaccuracyandcouldbethereforeusedfortheidentificationofSr22inwheatbreedingprograms.Theresultsindicatedthat0genotypecarriedtheresistancegeneamongthe58improvedresistantUg99wheatcultivarsfromabroadanddomestic,and3genotypescarriedtheresistancegeneamongthe18maincultivarsofHeilongjiang,withafrequencyof4.0%.ItreflectedthatSr22occupiedacertainproportioninHeilongjiangmainplantcultivarsanditshouldbecontinuetostrengthenuseofSr22inwheatbreedingprograminthefuture.

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备注/Memo

收稿日期：2012-12-03
基金项目：国家小麦现代产业技术体系东北综合试验站资助项目(nycytx-03)；黑龙江省科技攻关专项资助项目（GA09B101-4）
作者简介：马勇（1967-），女，黑龙江省克山县人，硕士，高级农艺师，从事小麦遗传育种研究。E-mail:mayong000@sina.com。

更新日期/Last Update: 2013-12-31