CAO Hui-mei,ZHANG Yue-xue,LIU Feng-qi,et al.Effect of Colchicine on Root Tip Chromosomein Zhaodong Alfalfa[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(05):121-124.
秋水仙素对肇东苜蓿根尖细胞染色体的影响
- Title:
- Effect of Colchicine on Root Tip Chromosomein Zhaodong Alfalfa
- 文章编号:
- 1002-2767（2012）05-0121-04
- 分类号:
- S551+.7
- 文献标志码:
- A
- 摘要:
- 为了探究秋水仙素对肇东苜蓿根尖细胞染色体的影响，利用不同浓度的秋水仙素（0.01%、0.05%、0.10%、0.15%和0.20%）以及处理时间（12、24、36和48 h）分别对肇东苜蓿根尖细胞进行诱导，观察肇东苜蓿根尖细胞染色体、细胞中期分裂指数和细胞加倍指数的变化。结果表明：秋水仙素诱导了肇东苜蓿根尖细胞染色体的变异，细胞内染色体加倍，数目不等(32、64及128条)；在时间与浓度互作方面，秋水仙素溶液浓度为0.15%时处理肇东苜蓿根尖12 h，使细胞中期分裂指数达到最大值18.51%；利用浓度为0.10%的秋水仙素处理24 h，细胞加倍指数达到最高值11.57%。
- Abstract:
- In order to understand the effect of colchicine on root tip chromosome of Zhaodong alfalfa,root tip cells of Zhaodong alfalfa were induced by different colchicines concentration(0.01%,0.05%,0.10%,0.15%,0.20%)and treatment duration(12,24,36,48 h).The change ofchromosome,mitosis index and cell doubling index of Zhaodong alfalfa root tip cell were observed.The results showed that colchicine induced variation in Zhaodong alfalfa root tip cell chromosome doubling and the observed chromosome number was 32,64 and 128.The metaphase mitotic index was as high as 18.51% when root tip cell was treated for 12 hours in 0.15% colchicine.The cell doubling index reached 11.57% as tip cell was treated for 24 hours in 0.10% colchicine.
参考文献/References:
[1]
杨恒山
,
曹敏建
,
范富
,
等
.
紫花苜蓿生长年限对土壤理化性状的影响
[J].
中国草地学报
,2006,28(6):29
-
32.
[2]杨起简,周禾,孙彦.紫花苜蓿的愈伤组织的诱导及组织培养[J].北京农学院学报,2004,19(2):28. [3]李世林,杨舸,易秀莉,等.根癌农杆菌介导的盐生杜氏藻DsNRT2基因转化紫花苜蓿的初步研究[J].四川大学学报:自然科学版,2004,45(2):409. [4]黎茵,黄学林,肖洁凝,等.根癌农杆菌介导苜蓿体胚转化及转基因植株再生[J].中山大学学报:自然科学版,2004,43(4):79. [5]肖艳云,杨恒山,张宏宇,等.磷酸二氢钾叶面不同喷施时期对紫花苜蓿的影响[J].中国草地学报,2008,30(1):61-65. [6]Evans G M.Polyploidy and crop improvement[J].J.Agric.Sot.niv.Wales,1981,62:93-l16. [7]秦素平,陈于和,林小虎,等.秋水仙素处理对黑麦根尖细胞有丝分裂的影响[J].麦类作物学报,2006,26(4):142-144. [8]王桂花,云锦凤,米福贵.秋水仙素诱导巨大赖草染色体加倍的研究[J].内蒙古草业,2006,18(1):6-8,12. [9]Geoffrian E,Kahane R.Ploidy stability and in vitrochromo-some doubling in gynogenic clone of onion[J].Plant Science,Limerick,1997,122(20):201-208. [10]宋智青,梁运章,李玉峰,等.药用植物的多倍体育种[J].生物学通报,2006,41(7):13-14. [11]李懋学,张赞平.作物染色体及其研究技术[M].北京:中国农业出版社,1996. [12]Chakraborti S P,Vijayan K.in vitroinduction of tetra-ploidy in mulberry(MorusalbaL.)[J].Plant Cell Reports,1998,17(10):799-803. [13]彭尽晖,张良波,彭晓英，等.秋水仙素在植物倍性育种中的应用进展[J].湖南林业科技，2004（5）：22-25. [14]齐志广,秘彩莉,柏峰，等.不同预处理对有丝分裂的影响[J].河北师范大学学报：自然科学版,2003,27(1):88-91. [15]李光明,刘文海,何波.四种不同处理对蚕豆根尖细胞有丝分裂的影响[J].湘潭师范学院学报：自然科学版,2005,27(1):82-83. [16]黄永莲,胡木林.秋水仙素对洋葱根尖细胞的诱变效应[J].亚热带植物学报,2005,34(1):42-45. [17]孔舒颖,王照兰,杜建材,等.扁蓿豆新品系的多倍体诱导研究[J].中国草地学报，2010（4）：31-34. [18]马育华.田间试验与统计分析[M].北京:中国农业出版社,1978. [19]连雪斌.兰州百合多倍体诱导试验报告[J].甘肃农业科技,1995(6);14-15. [20]吴丽芳，张鸭关.紫花苜蓿离体组织染色体加倍技术及在育种中的应用[J].草业与畜牧,2009（5）：7-9. [21]Chase S C.Analytical breeding of Solanum Tubersum[J].Can.J.Gen.1963,5:359-363. [22]Wenzel G O.Comparison of single cell culture derived Sodanum Tubersum plant and model for their application in breeding programs[J].Theor.Appl.Genet,1979,55:49-55. [23]于品华，曲秀兰，戴朝曦.用秋水仙素对马铃薯-单倍体和双倍体染色体加倍的研究[J].甘肃农业大学学报,1991,26(3):264-269.
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备注/Memo
基金项目：农业科技成果转化资金资助项目（2011GB2B200005）；黑龙江省农业科技创新工程种子创新基金资助项目（2010-10）