ZHAO Yun-tong.Optimization of Fermentation Condition ofBacillus laterosporus HBL-26[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(05):112-116.
侧孢芽孢杆菌HBL-26发酵条件的优化研究
- Title:
- Optimization of Fermentation Condition ofBacillus laterosporus HBL-26
- 文章编号:
- 1002-2767（2012）05-0112-05
- Keywords:
- Bacillus laterosporus; fermentation condition; optimization
- 分类号:
- TQ920.6
- 文献标志码:
- A
- 摘要:
- 为对拮抗菌株HBL-26的发酵培养基进行优化，通过正交试验设计对发酵液的4个单因子进行优化筛选，经软件分析后确定发酵培养基最佳配方为：葡萄糖6 g·L-1、牛肉膏14 g·L-1、NaCl 6 g·L-1、MnSO4?0.05 g·L-1。同时，得到回归方程：Y=5.34X1-1.72X2+14.44X3-6.43X4+8.35，其标准差S=14.23；F=4.202；显著值Sig.=0.019<0.05。最后通过单因子试验对菌株HBL-B26的发酵条件进行了优化，确定的最优培养条件为：培养基初始pH 8、发酵温度30℃、发酵最佳时间为48 h。
- Abstract:
- In order to optimize the fermentation culture medium of HBL-26,four single factors were selected by the orthogonal design.The optimum medium was glucose 6 g·L-1,beef extract14 g·L-1,NaCl 6 g·L-1and MnSO4 0.05 g·L-1.The regression model was obtained Y=5.34X1-1.72X2+14.44X3-6.43X4+8.35.S=14.23;F=4.202;Sig=0.019<0.05.Four factors were tested to increase the antibiotics yield by single-factor test method.The result showed that the optimum process parameters were initial pH was 8.0,culture temperature was 30℃ and the best fermentation time was 48 h.
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备注/Memo
作者简介：赵云彤(1983-)，女，黑龙江省双鸭山市人，硕士，研究实习员，从事微生物和植物保护研究。E-mail：zyt-37@163.com。