LIU Chang-bin,NI Jian-hong,QU Ming-yue,et al.Application Effect of Frozen Sexing Semen on MuttonSheep Embryos Transformation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(04):82-86.
性控冻精在肉羊胚胎移植中应用效果的研究
- Title:
- Application Effect of Frozen Sexing Semen on MuttonSheep Embryos Transformation
- 文章编号:
- 1002-2767（2012）04-0082-05
- Keywords:
- mutton sheep; frozen sexing semen; superovulation; laparoscope
- 分类号:
- S826.9+2
- 文献标志码:
- A
- 摘要:
- 为进一步研究肉羊性控精液在胚胎生产上的应用，以萨福克、无角陶赛特纯种肉用绵羊为供体，本地小尾寒羊杂交后代和湖羊为受体，利用子宫角深部输精及一般人工授精等辅助生殖技术使用性控冻精与普通鲜精对两品种肉羊进行人工授精，统计不同品种肉羊的受胎率和可用胚数，对获得的性控胚胎和普通胚胎进行移植，并对比其移植效果。结果表明：萨福克肉羊和无角陶赛特肉羊超排后黄体数差异不明显（P＞0.05）；普通精液输精所获得的胚胎数极显著高于性控冻精（P＜0.01）；在受胎率上也做了比较，但差异不显著，受胎率43.2%。由于精液在通过流式细胞仪时，对精子顶体造成一定损伤，因此，无论是子宫角输精或阴道子宫颈输精均影响受精率，但性控精液的推广应用确实可以加速家畜遗传育种速度，缩短育种周期。鉴于此，今后对性控精液的研究重点应该放在精液分离过程中对精子的保护，以减少对精子的损伤。
- Abstract:
- The donors were Suffolk and Poll Dorset,recipients were Small Tail Han Sheep and Hu Sheep.The frozen sexing semen and normal fresh semen were used in insemination of donors.The fertilization rate and available embryos number of these two kinds of semen were compared in order to provide reference for the further application of frozen sexing semen in embryo production.Endoscope insemination and normal artificial insemination of those two kinds of semen were applied in embryo production of two mutton sheep breeds;the fertilization rate and available embryos number of those two mutton sheep breeds were compared,and the transfer effect of sexing embryos and normal embryos were compared as well.The results showed that the number of corpus luteum of those two breeds after superovulation showed no difference (P＞0.05).The available embryos number from normal fresh semen was several significant higher than that from frozen sexing semen (P＜0.01).The pregnancy rate for the recipients was 43.2% and showed no difference.However,the frozen sexing semen negatively affected fertilization rate of donors because of the sperm acrosome damage after the sperm processing in flow cytometry,the application of sexing semen could improve breeding process.Therefore,in the future research,the hot topic would be how to protect sperm while applying the X/Y sperm selection.
参考文献/References:
[1]黄金明，游伟，张俊功，等.性控精液在奶牛生产中的应用策略[J].动物医学进展，2006，27（1）：100-104. [2]李强，牛志红，刘水涛，等.常规精液与性控精液的超数排卵效果比较[J].中国奶牛，2010(7):37-38. [3]Catt S L,Sakka D,Bizzaro D,et al.Hoechst staining and exposure to UV laser during flow cytometric sorting does not affect the frequency of detected endogenous DNA nicks in abnormal and normal spermatozoa[J].Molecular Human Reproduction,1997(3):821-825. [4]Johson L A.Sexing mammalian sperm for the production of offspring:The state-of-the-art[J].Anim.Reprod Sci.,2000,61:93-107. [5]Johson L A,Welch G R,Rens W.The Beltsville sperm sexing technology:High-speed sorting gives improved sperm output for in vitro fertilization and AI[J].J.Anim.Sci.,1999,77:213-220. [6]Seidel G E J,Schenk J L.Feild trial with sexed,frozen bovie semen[R]// Proc 19th Tech.Conf on Artificial Insemination and Reproduction.Columbia,Missouri:Natl Assn of Animal Breeders Columbia,2002. [7]罗应荣，黄河，李鑫，等.奶牛胚胎性别控制技术的试验研究进展[J].中国畜牧兽医，2005，32：32-35. [8]王敬军，胡大伟，刘国辉，等.FSH剂量、受体品种及移植胚胎数对崇明白山羊胚胎移植效率的影响[J].上海交通大学学报：农业科学版，2011，29（1）：38-42. [9]王锋，代相鹏.激素对波尔山羊超排效果影响的研究[J].畜牧与兽医，2006，38（5）：8-10. [10]赵振华，周敏敏，钱红娟，等.山羊超数排卵效果的影响因素分析[J].上海畜牧兽医通讯，2008（1）：48-49. [11]Keith J B.A history of farm animal embryo transfer and some associated techniques[J].Animal Reproduction Science,2003,79:203-244. [12]朱士恩.动物生殖生理学[M].北京：中国农业出版社，2006. [13]Stocco D M.Star protein and the regulation of steroid hormone biosynthesis[J].Annu Rep Physio1.,2001,63:193-213. [14]张嘉保，周虚.动物繁殖学[M].北京：吉林科学技术出版社，1999. [15]石国庆.绵羊繁殖与育种新技术[M].北京：金盾出版社，2010. [16]Rubianes E,Teresa D E Castro,Kmaid S,et a1.Superovulatory response to FSH treatments aftert different progesterone primings in ewes[J].Small Ruminant Research,1999,33:156-164. [17]雷赵民，吴建平，Martin Wenkoff.绵羊胚胎移植生殖内分泌调控及其效果研究[J].草业科学，2009，26（6）：126-131. [18]Johnson L A,Flook J R,Look M V.Flow cytometry of X and Y chromosome-bearing sperm for DNA using an improved preparation method and staining with Hoechst33342[J].Gamete Research,1987,17:203-212. [19]Johnson L A,Flook J P,Hawk H W.Sex preselection in rabbits:live births from X and Y sperm separated by DNA and cell sorting[J].Biology of Reproduction,1989,41:199-203. [20]Baril F,Khalid M,Haresign W,et al.Factors affecting the survival of sheep embryos after transfer within a MOET Program[J].Theriogenology,2003，59:1265-1275. [21]代相鹏，张秀华，王锋.波尔山羊胚胎移植影响因素的研究[J].家畜生态学报，2007，28（6）：58-63. [22]高志敏，雷安民，窦忠英，等.波尔山羊胚胎移植技术的研究与应用[J].农业生物技术学报，2001，9（2）：167-170. [23]李俊杰，桑润滋，金东航，等.影响波尔山羊胚胎移植产羔效果因素研究[C]//窦忠英,陈宏.全国首届动物生物技术学术研讨会论文集.杨凌：西北农林科技大学，2004. [24]苏和.内蒙古克什克腾旗绵羊鲜胚移植技术应用研究[J].内蒙古畜牧科学，1999（3）：37-39. [25]朴庆林，王晓阳，金香淑.绵羊冷冻胚胎移植试验报告[J].吉林农业科学，1996（3）：86-87. [26]Armstrong D T.Factors affecting superovulation success[J].Embryo Transfer Newsletter,1991(9):11-17. [27]王光亚，段恩奎.山羊胚胎工程[M].西安：天则出版社，1993. [28]李俊杰，桑润滋，金东航，等.提高波尔山羊胚胎移植妊娠率的研究[J].中国畜牧杂志，2005，41（10）：18-20. [29]王玉琴，赵有璋，李发弟，等.提高肉用绵羊胚胎移植成活率的研究[J].中国畜牧杂志，2007，43（9）：16-18.
备注/Memo
基金项目：“十一五”国家科技支撑计划资助项目（2010BAD05B012）；国家“863”计划资助项目（2011AA100307）；兵团国际科技合作资助项目（2010YD39）；南疆科技专项资助项目（2011BA061）