[1]曹士亮,史桂荣,王成波,等.转BcWRKYⅠ基因玉米杂交组合苗期耐盐性分析[J].黑龙江农业科学,2012,(04):23-27.
　CAO Shi-liang,SHI Gui-rong,WANG Cheng-bo,et al.Analysis on Salt Tolerance of Transgenic Maize(Zea mays L.)Crosses with BcWRKYⅠGene in Seedling Stage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(04):23-27.
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转BcWRKYⅠ基因玉米杂交组合苗期耐盐性分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2012年04 页码: 23-27 栏目: 出版日期: 2012-04-10
Title:
Analysis on Salt Tolerance of Transgenic Maize(Zea mays L.)Crosses with BcWRKYⅠGene in Seedling Stage
文章编号:
1002-2767（2012）04-0023-05
作者:
曹士亮1; 史桂荣1; 王成波1; 贺强1; 蔡泉1; 卢翠华2; 林忠平3
1.黑龙江省农业科学院 玉米研究所，黑龙江 哈尔滨 150086；
2.东北农业大学 农学院，黑龙江 哈尔滨 150030；
3.北京大学 生命科学学院，北京 100083
Author(s):
CAO Shi-liang1; SHI Gui-rong1; WANG Cheng-bo1; HE Qiang1; CAI Quan1; LU Cui-hua2; LIN Zhong-ping3
1.Maize Research Institute of Heilongjiang Academy of Agricultural Sciences，Harbin，Heilongjiang 150086；
2.Agronomy College of Northeast Agricultural University，Harbin，Heilongjiang 150030;
3.Life Science College of Beijing University,Beijing 100083
关键词:
转基因玉米; 苗期; 耐盐性
Keywords:
transgenic maize; seedling stage; salt resistance
分类号:
S513.035.3
文献标志码:
A
摘要:
为了明确外源基因提高玉米耐盐性的效果，以6份转BcWRKYⅠ基因的玉米杂交组合及其对应的6份非转基因对照组合为试材，对其发芽率、耐盐级别、株高、干重、脯氨酸含量等7个指标进行分析。结果表明：耐盐级别、发芽率、干重、叶绿素含量和相对电导率的变化均达到显著水平，株高的变化达到极显著水平，脯氨酸含量转基因与非转基因组合间差异不显著。
Abstract:
In order to analyze the effect of salt tolerance improvement by exogenous gene transformation，six transgenic maize crosses with BcWRKYⅠ gene and six respective non-transgenic control of them were used to identify salt toleranc in seedling stage.Seven indicators such as the germination rate,salt resistance level,plant height,dry weight,proline content and so on were analyzed to evaluate the effect of the transformation of BcWRKYⅠ gene on improving the salt resistance.The results indicated that salt resistance level,germination rate,dry weight,chlorophyll content,relative electric conductivity appeared significant differences,plant height appeared extremely significant differences,however,the difference of proline content was not significant.

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备注/Memo

基金项目：农业部转基因重大专项资助项目（2009ZX08003-015B）

第一作者简介：曹士亮（1980-），男，黑龙江省哈尔滨市人，硕士，助理研究员，从事玉米生物技术与遗传育种研究。E-mail:caoshiliang2003@126.com。

更新日期/Last Update: 2014-10-26