[1]刘祥伟.意蜂三型蜂间哺育王浆蛋白组分差异的研究[J].黑龙江农业科学,2012,(01):60-63.
LIU Xiang-wei.Difference in Protein Composition ofBreeding Jelly in Apis mellifera ligustica [J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(01):60-63.
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LIU Xiang-wei.Difference in Protein Composition ofBreeding Jelly in Apis mellifera ligustica [J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(01):60-63.
意蜂三型蜂间哺育王浆蛋白组分差异的研究
《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S]
卷:
期数:
2012年01
页码:
60-63
栏目:
出版日期:
2012-01-10
- Title:
- Difference in Protein Composition ofBreeding Jelly in Apis mellifera ligustica
- 文章编号:
- 1002-2767（2012）01-0060-04
- Keywords:
- Apis mellifera ligustica; royal jelly; SDS-PAGE; protein composition
- 分类号:
- S896.3
- 文献标志码:
- A
- 摘要:
- 为研究蜜蜂三型蜂幼虫期间食物的差异，现对意大利蜜蜂三型蜂间哺育王浆蛋白组分的差异性进行研究。对工蜂幼虫哺育浆、雄蜂幼虫哺育浆、蜂王幼虫哺育浆分别用水和磷酸缓冲液进行提取，利用聚丙烯酰胺凝胶电泳分析。结果表明：工蜂和雄蜂各日龄幼虫哺育浆间存在不同程度的差异；各个日龄蜂王幼虫哺育浆的蛋白质谱带基本相同；哺育浆分别经水和磷酸缓冲液提取，结果存在差异。
- Abstract:
- In order to study the food difference between three types of bee during larva period,the difference of royal jelly protein component bred by three types of Apis mellifera ligustica were studied. The protein component of breeding jelly extracted by water and PBS,respectively from the worker,drone and queen of were analyzed by SDS-PAGE.The results showed that there were differences in protein components of jelly bred by various instars of worker and drone.The protein bands were no difference in jelly fed by various instars of queen.The protein bands were different in jelly extracted by water and PBS.
参考文献/References:
[1]
陈崇羔，陈婉玉，龚蜜
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蜂王浆中水溶性蛋白质分子量的探讨
[J].
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[2]宋卫中,宋晓勇，刘蔚.蜂王浆的研究和应用综述[J].中华实用中西医杂志，2005，18(6)：917-919. [3]朱黎.蜂王浆中的蛋白质[J].蜜蜂杂志，2005（5）：30-31. [4]方国桢，方建生，田树革.蜂王浆成分及其分析方法研究进展[J].中国乳品工业，1994(6)：69. [5]杨远帆，陈靖刚，倪辉.蜂王浆中蛋白质及肽类物质的研究进展[J].中国养蜂,2004（4）：27-28. [6]Fujiwara S,Imai J,Fujiwara M，et al.A Potent antibacterial Protein in royal jelly[J].J.Biol.Chem，1990，265(19):11333-11337.
备注/Memo
基金项目：现代农业产业技术体系（蜜蜂）资助项目（CARS-45-SYZ5）作者简介：刘祥伟（1983-），男，山东省临沂市人，学士，研究实习员，从事蜂学研究。E-mail:laoniu_1107@163.com。
更新日期/Last Update:
2014-10-25