ZHANG Dong-jie.Porcine MC4R Gene Haplotypes Detection and Analysis[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(12):10-13.
猪黑素皮质素受体4基因的单倍型检测与分析
- Title:
- Porcine MC4R Gene Haplotypes Detection and Analysis
- 文章编号:
- 1002-2767(2011)12-0014-02
- 关键词:
- 猪; 黑素皮质素受体4基因; 测序; 单倍型
- 分类号:
- S828;Q78
- 文献标志码:
- A
- 摘要:
- 黑素皮质素受体4(melanocortin 4 receptor,MC4R)是G蛋白耦联受体超家族的一个成员,在人和许多动物的体重、能量稳态和采食量的调控中发挥重要作用。现采用直接克隆测序的方法对23头长白猪、25头大白猪、19头民猪和7头野猪的MC4R基因进行了测序与分型。共检测到SNP位点72个,其中发生频率在2次以上的SNP位点有6个,有3个为错义突变,引起了多肽链氨基酸的替代;6个SNP位点共组成了14种单倍型,其中H1、H3、H5、H6、H7、H9、H11和H14为首次发现的单倍型。
- Abstract:
- Melanocortin-4 receptor(MC4R)is a member of the superfamily of G protein coupled receptors,which affects body weight,energy homeostasis and food intake in human and many animals.The coding region of MC4R gene in 23 landrace,25 large white pig,19 Min pig and 7 wild boar were investigated using clone sequence method.The results showed that 72 SNPs were found,the frequency in which happened more than twice were 6,3 were missense mutation,caused the substitution of amino acids polypeptide chain.6 SNPs composed 14 haplotype,H1,H3,H5,H6,H7,H9,H11 and H14 were first found.
参考文献/References:
[1]杨秀芹,于浩,顾志刚,等.野猪MC4R基因的克隆及变异初步研究[J].动物学杂志,2007,42(2):71-74. [2]张金伟,陈代文,李云兰.黑素皮质素受体系统在动物采食调控中的作用机制及研究进展[J].饲料博览:技术版,2009(7):7-11. [3]杨晓慧,刘源,唐辉,等.猪MC4R基因Asp298Asn位点的多态性及其与商品猪背膘厚的关系[J].农业生物技术学报,2008,16(3):407-411. [4]D’Andrea M,Pilla F,Giuffra E,et al.Structural analysis and haplotype diversity in swine LEP and MC4R genes[J].J.Anim Breed Genet,2008,125(2):130-136. [5]杜红丽,崔建勋,张细权.利用DNA池和测序技术快速筛查候选效应单倍型[J].农业生物技术学报,2008,16(2):281-285.
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备注/Memo
作者简介: 张冬杰(1980-),女,黑龙江省佳木斯市人,博士,助理研究员,从事猪的分子遗传育种研究。E-mail:djzhang8109@163.com。