[1]赵 茜,徐丽珍.快速提取籽用南瓜基因组DNA方法的研究[J].黑龙江农业科学,2011,(10):14-15.
　ZHAO Qian,XU Li-zhen.A Rapid Method for Extraction Genomic DNA from Seed Squash[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(10):14-15.
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快速提取籽用南瓜基因组DNA方法的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2011年10 页码: 14-15 栏目: 出版日期: 2011-10-10
Title:
A Rapid Method for Extraction Genomic DNA from Seed Squash
文章编号:
1002-2767（2011）10-0014-03
作者:
赵 茜; 徐丽珍
黑龙江省农业科学院 经济作物研究所，黑龙江 哈尔滨 150086
Author(s):
ZHAO Qian; XU Li-zhen
Industrial Crops Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086
关键词:
籽用南瓜; DNA; 提取方法
Keywords:
seed squash; DNA; extraction method
分类号:
S642.1
文献标志码:
A
摘要:
为快速高效提取作物基因组DNA,以南瓜品种为试材，对籽用南瓜基因组DNA的快速、高效提取方法进行研究，并对产物进行紫外分光光度计和琼脂糖凝胶电泳分析。结果表明：通过对CTAB改良后提取的基因组DNA结构较完整，杂质较少，浓度较高，可经纯化直接用于进一步的PCR等分子生物学研究。
Abstract:
In order to extract DNA fast and efficient,the seed squash was taken as materials to study the extraction method.It was analyzed by ultraviolet spectrophotometer and agarose gel electrophoresis.The results showed that structure of DNA was complete but had less impurities,its concentration was high,it could be directly used for the further study of molecular biology(such as PCR).

参考文献/References:

[1]于红茹 . 我国籽用南瓜现状及前景分析 [J]. 北方园艺 ,2001(6) ： 54.

[2]谭行之,崔崇士,屈淑平.南瓜作物分子标记辅助育种研究进展[J].中国瓜菜,2010,23(3)：37-40.

[3]刘维侠，曹振木，王秀全.彩色甜椒基因组 DNA 提取方法的优化[J].热带农业科学,2010,30(2)：19-23.


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备注/Memo

第一作者简介：赵茜（1982-），女，内蒙古自治区乌兰察布市人，硕士，研究实习员，从事籽用南瓜病害及遗传育种研究。E-mail：zhaoqian0401@sina.com。通讯作者：徐丽珍（1963-），女，黑龙江省双城市人，副研究员，从事籽用南瓜遗传育种及栽培技术研究。E-mail：jzsxulizhen@163.com。

更新日期/Last Update: 2014-11-01