SHA Wei,HE Hai-yan,ZHANG Yan-fu.The Isozyme Analysis of Some Soybean Varieties from Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(10):1-4.
黑龙江省25份大豆品种的同工酶分析
- Title:
- The Isozyme Analysis of Some Soybean Varieties from Heilongjiang Province
- 文章编号:
- 1002-2767(2011)10-0001-04
- Keywords:
- soybean; isozyme; cluster analysis
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 采用聚丙烯酰胺凝胶电泳技术,对供试25份大豆品种的过氧化物同工酶(Peroxidase isozyme,简称POD)、酯酶同工酶(Esterase isozyme,简称EST)、超氧化物歧化酶(Superoxide dismutase,简称SOD)进行分析。结果表明:品种间的POD、EST、SOD同工酶酶谱在酶带数、迁移率及酶活性等方面均较相似,同时存在一定的差异。3种同工酶共分离出23条带,其中多态性条带13条,多态性比率为56.52%;25个品种间的相似性系数变幅范围为0.56~0.95,平均值为0.76。说明黑龙江省大豆种质资源间存在一定的遗传变异性。UPGMA聚类分析结果表明:在GS = 0.8处,供试材料共划分为5个类群。
- Abstract:
- Polyacrylamide gel electrophoresis(PAGE)was adopted to study Peroxidase isozyme,Esterase isozyme and Superoxide dismutase of 25 soybean varieties from Heilongjiang province.The results showed that zymograms of these POD,EST,SOD of 25 soybean varieties were similarly but with some differences in terms of band number,mobility and isozymes activity(enzyme with depth).A total of 23 bands were detected in 25 cultivars using three isozymes markers,the polymorphic bands were 13,amount to 56.52%,statistical results revealed that the similarity coefficient among the 25 varieties ranged from 0.56 to 0.95,and the average was 0.76.It suggested that there were genetic variations among the varieties.According to the cluster analysis,the tested materials could be divided into 5 groups at the level GS 0.8.
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备注/Memo
基金项目:黑龙江省教育厅科研资助项目(10551331)第一作者简介:沙伟(1963-),女,黑龙江省人,教授,博士生导师,从事植物学和植物遗传学方面的研究。E-mail:Shw1129@263.net。