[1]洪森荣,陈子敏.糖类和琼脂对黄独生长发育和离体保存的影响[J].黑龙江农业科学,2011,(08):23-24.
HONG Sen-rong,CHEN Zi-min.Effect of Carbohydrate and Agar on the Growth and Developmentand Conservation in vitro of Dioscorea bulbiferaL.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(08):23-24.
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HONG Sen-rong,CHEN Zi-min.Effect of Carbohydrate and Agar on the Growth and Developmentand Conservation in vitro of Dioscorea bulbiferaL.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(08):23-24.
糖类和琼脂对黄独生长发育和离体保存的影响
《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S]
卷:
期数:
2011年08
页码:
23-24
栏目:
出版日期:
2011-08-10
- Title:
- Effect of Carbohydrate and Agar on the Growth and Developmentand Conservation in vitro of Dioscorea bulbiferaL.
- 文章编号:
- 1002-2767(2011)08-0023-02
- 分类号:
- S567.239
- 文献标志码:
- A
- 摘要:
- 为加强黄独的组织培养研究,以黄独无菌苗为试材,研究了糖类(葡萄糖、蔗糖和白砂糖)和不同琼脂浓度对黄独生长发育和离体保存的影响。结果表明:液体培养基有助于黄独的生长发育,而固体培养基有助于黄独的离体保存。蔗糖和白砂糖有利于黄独的生长发育,而葡萄糖有利于黄独的离体保存。
- Abstract:
- In order to strengthen study for tissue cultuge of Dioscorea bulbifera L..Taking germ-free plantlets of Dioscorea bulbifera L.as experimental material,the effect of carbohydrate(glucose,sugar and white sugar)and different agar concentration on the growth and development and conservation in vitro of Dioscorea bulbiferaL.plantlets were studied.The results showed that the liquid culture medium could promote the growth of Dioscorea bulbifera L.plantlets,while the solid culture medium contributed to conservation in vitro of Dioscorea bulbifera L.plantlets;Sugar and white sugar were beneficial to the growth and development of Dioscorea bulbifera L.plantlets,but glucose contributed to conservation in vitro of Dioscorea bulbifera L.plantlets.
参考文献/References:
[1]李石生,邓京振,赵守训.黄独块茎的甾体类成分[J].植物资源与环境,1999,8 (2):61-62. [2]孙伟,李敬,马淑坤.东北地区栽种黄独技术要点[J].特种经济动植物,2007(4):41. [3]郑玉红,夏冰,杭悦宇,等.黄独遗传多样性研究[J].西北植物学报,2006,26(10):2011-2017. [4]张丽杰,陈晓旭,周强,等.光照、琼脂和碳源对桃叶卫矛腋芽离体培养的影响[J].辽宁林业科技,2009(1):31-34. [5]兰伟,陈素梅,尹冬梅,等.那贺川野菊的离体保存[J].园艺学报 2010,37(12):2007-2016.
备注/Memo
基金项目:江西省教育厅2011年度一般科技资助项目(GJJ11610)第一作者简介:洪森荣(1974-),男,江西省永新县人,硕士,副教授,主要从事植物生物技术方面的研究。E-mail:hongsenrong@163.com。
更新日期/Last Update:
2014-11-05